Figures and Table for Supplemental material

Kemin Zhou

Nov 20, 2011

Transcription overlap

Number of genes per congregation

Genome Agabi2 Aspca3 Spoth2 Lacbi2 Aspac1

%Multi genes 13.5 38.1 63.0 44.5 63.9

%Genes in Cong. 25.4 63.5 87.4 71.8 90.9

Cou

nt

Fig S1

Fig S2

Relative Expression Level of Minor Isoforms

Co

un

t

Agabi2

0.0 0.4 0.8

050

015

00

Aspca3

0.0 0.4 0.8

020

0060

00

Spoth2

0.0 0.4 0.8

020

0060

00

Lacbi2: Sanger+454

0.0 0.4 0.8

020

040

060

0 Lacbi2

0.0 0.4 0.8

010

000

3000

0

Aspac1

0.0 0.4 0.8

020

000

4000

0

100 200 300 400

0.2

50

.30

0.3

50

.40

0.4

50

.50

SO

M (

std

/me

an

)

Average EST Length (nt)

81.08797.2162.0xxy

Agabi2Aspca3

Aspac1

Spoth2

Lacbi2

Fig S3

Agabi2 N

AG

Fig S4

NA

G

Aspca3

Fig S5

NA

GN

AG

Lacbi2

Fig S6

NA

GN

AG

Spoth2

Fig S7

NA

GN

AG

Aspac1

Fig S8

NA

GN

AG

0.0

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

AD AACE

CE_AD

CE_AA

CE_ADAA IR

ADAA

CEorAAen

dM

E

Other

Agabi2

Aspca3

Spoth2

Lacbi2

Aspac1

AS Category

Fra

ctio

n of

Tot

al

Fig S9

0.00

0.01

0.02

0.03

0.04

CE

CE_AD

CE_AA

CE_ADAA

CEorAAen

d ME

Other

A

B

E

D

C

Fig S10

Intron Length (nt)

Fre

qu

en

cy

Intron Length (nt)

Major: Expression Level > median of complete

models Major isoform of AS

All: Intron from partial + complete models

0.26

0.28

0.3

0.32

0.34

0.36

0.38a

ll

ma

j

ma

jS

ma

jL all

ma

j

ma

jS

ma

jL all

ma

j

ma

jS

ma

jL all

ma

j

ma

jS

ma

jL all

ma

j

ma

jS

ma

jL

Agabi2 Aspca3 Lacbi2 Spoth2 Aspac1

3n 3n+1 3n+22.

6E-0

9

0.02

163

0.95

605

0.41

517

5.1E

-05

0.11

912

0.32

093

0.03

850

0.00

014

6.6E

-06

0.68

033

0.00

084

0.02

261

0.53

449

0.05

320

0.07

668

0.11

337

0.77

613

0.01

756

0.02

417

Fig S11

Fra

ctio

n