VECTORS 30-12-2012 1 30-12-2012 3 30-12-2012 4 • The artificial plasmid pUC18 has been genetically engineered to include a gene for antibiotic resistance to Ampicillin…
Slide 1 Slide 2 Introduction Recombinant DNA technology has revolutionized biochemistry since it came into being in the 1970s. The genetic endowment of organisms can now…
Slide 1 Slide 2 1.Biochemical biomarkers Based on the ability of the pollutant to generate a response at the gene level, inducing or increasing specific enzymes involved…
Slide 1prepare foreign (target) DNA prepare vector (host) recombine target and vector DNA introduce rDNA to host screen for DNA of interest Recombinant DNA gDNA (fragments)…
1. CLONING Sidra Shafique Genes to genomes Pg 55-58 Genes and genomics Pg 68-74 2. 2.9.2. Cloning sites • The second necessary characteristic of a vector is a cloning site,…
Slide 1 Chp 5 Introduction of DNA into Living Cells Huseyin Tombuloglu PhD. GBE310, Spring 2015 Slide 2 Chp 5 Introduction of DNA into Living Cells Slide 3 Cloning serves…
Slide 1 Genregulation Literature - Alberts/Lehninger - Kim Sneppen & G. Zocchi: Physics in Molecular Biology - E. Klipp et al. : Systems Biology in Practice Systems biophysics…
Slide 1 Sequencing a genome and Basic Sequence Alignment Lecture 10 1Global Sequence Slide 2 Annotation of sequences Once the gene sequence’s have been determined then…
Slide 1 The Effect of DNA Methylation on Deamination Rate and Mutagenesis Tuyen T. Dang Mentor: Dr. Christopher K. Mathews Biochemistry/Biophysics Analysis of Ribonucleotide…