CHAPTER 17 APPLICATIONS OF rDNA TECHNOLOGY IN AGRICULTURE: PRODUCTION OF TRANSGENIC PLANTS For several thousand years, farmers have been altering the genetic makeup of the…

Agrobacterium tumefaciens Agrobacterium tumefaciens is a ubiquitous soil borne pathogen responsible for Crown Gall disease (right), affecting many higher species of plant.…

Recombinant DNA Technology 12. Recombinant DNA Technology Key Concepts Recombinant DNA is made by splicing a foreign DNA fragment into a small replicating molecule (such…

Particle Bombardment Biological Instrumentation Miss.R.Uma Dept. of Biotechnology FAPM, WUSL Introduction  Transformation is the genetic alteration of a cell resulting…

VECTORS 30-12-2012 1 30-12-2012 3 30-12-2012 4 • The artificial plasmid pUC18 has been genetically engineered to include a gene for antibiotic resistance to Ampicillin…

1. RECOMBINANTDNATECHNOLOGY 2. What is Genetic Engineering? Genetic engineering (GE) is the transfer of genes from one organism to another through means that do not occur…

LIGATION SUJIT KUMAR FB-MA2-01 LIGATION OF TARGET DNA AND VECTOR vector insert ligase LIGASE • JOINING OF NUCLEIC ACID EITHER DNA OR RNA- THROUGH PHOSPHODIESTER BOND. •…

DNA Vaccine Coding for prevention……. Coding against immunity…… What are vaccines?????  Vaccine is an attenuated form of disease causing organism, able to stimulate…

RECOMBINANT RECOMBINANT PROTEINS PROTEINS PRESENTED BY: ASH . K INTRODUCTION • Recombinant proteins are the proteins that are produced by genetically modified organisms…

TRANSGENIC ANIMALS INTRODUCTION: The term transgenic animal refers to an animal in which there has been a deliberate modification of the genome - the material responsible…