Liquid chromatography and electrospray mass spectrometric mapping of peptides from human plasma filtrate Manfred Raidaa, , , Peter Schulz-Knappea, Gabriele Heinea, Wolf-Georg Forssmanna a Lower Saxony Institute for Peptide Research, Hannover, Germany Received 10 December 1997 Revised 21 July 1998 Accepted 22 August 1998 Available online 15 July 1999 http://dx.doi.org/10.1016/S1044-0305(98)00117-2, How to Cite or Link Using DOI Permissions & Reprints
Q IT TOF FT OT FT ICR
Resolution - +/- + ++ +++
Mass Accuracy (ppm)
- - ++ ++ +++
Acquisition Speed (Hz)
+ + +++ +/- -
m/z range - + +++ + ++
Linearity ++ + + +/- +/-
Price +++ ++ +/- +/- -
Domon B, Aebersold R. Science 2006
Presentator
Presentatienotities
Mass Spectrometry and Protein Analysis Science 14 April 2006: �vol. 312 no. 5771 212-217
Holcapek et al. J Chromatography B 2012
Ionization: the Missing Link
Grebe SK, Singh RJ. Clin Biochem Rev 2011
Grebe SK, Singh RJ. Clin Biochem Rev 2011
Vogeser M, Seger C. Clin Chem 2010
Presentator
Presentatienotities
www.molecularstation.com
Holcapek et al. J Chromatography B 2012
Practical Example
• Develop a LC-MS/MS method for X
• How to start?
Sample Preparation
• Dilute and shoot • Protein precipitation • SPE • Liquid-Liquid • IAC