ABO Discrepancies & other problems cls.umc.edu/COURSES/CLS325/Week5/Discrepancies.ppt Reneé Wilkins, PhD, MLS(ASCP)CM CLS 325/435 School of Health Related Professions…

1.Advanced Aminal Cell Culture 2013 2nd Semester Department of Animal Science Chungbuk National University 2st Lecture 2. Syllabus Date Topics September 5, 2013 Introduction…

1. Laboratory Safety 2. Monolayer culture• Requires substrate or solid surface for attachment and growth : Anchorage dependent of growth• Contact inhibition of movement…

Slide 1 Tian He 2008. 07. 06 Slide 2 Media non-selective: YPD without antibiotics Selective: Synthetic complete dropout medium (SC) (auxotroph) YPD with antibiotics Slide…

Slide 1 DNA transfection Slide 2 Specific methods used For Transfection 1. Electroporation a brief change of electric pulse discharges across the electrode, transiently open…

Part 1. Production of Pure, Homogeneous and Stable Membrane Protein Larry Miercke, Rebecca Robbins, Mimi Ho, Andrew Sandstrom*, Rachel Bond, Bill Harries and Robert Stroud…

DNA transfection Specific methods used For Transfection 1. Electroporation  a brief change of electric pulse discharges across the electrode, transiently open holes in…

Part 1. Production of Pure, Homogeneous and Stable Membrane Protein Larry Miercke, Rebecca Robbins, Mimi Ho, Andrew Sandstrom*, Rachel Bond, Bill Harries and Robert Stroud…

- Purification of nuclei from 0-16 hrs embryos - UV cross-linking - in vivo IP with anti-EN Ab - Sonication (0.1 - 3 Kb) Addition of linkers PCR amplification PCR amplification.…