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TEKNIK ISOLASI PROTEIN  Protein ekstraseluler  Tahapan isolasi  Sentrifugasi cairan sel/ medium kultur  menghasilkan :  Supernatan (crude extract)  Pelet…

Education Proteinas

1. Spectroscopic Properties of Aminoacids • All amino acids absorb in infrared region • Only Phe, Tyr, and Trp absorb UV • Absorbance at 280 nm is a good diagnostic…

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Expression of Foreign Proteins in E. Coli -A Practical approach Navin Khanna Overview of a practical approach • • • • • • • • • • • Gene isolation:…

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Chapter 3 Centrifugation Biochemistry and Molecular Biology (BMB) 3.1 Introduction 3.2 Basic Principle of sedimentation 3.3 Types, care and safety of centrifuges 3.4 Preparative…

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Affinity Chromatography A Bioanalytical Tool Guided by: Mr. S.A.Pishwikar Sir. Asst. Professor. Presentation By: Mr. Arpit H. Patel. M.Pharm 1st sem. Department of Quality…

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UNIVERSITY OF OTTAWA Faculty of Science Biochemistry BCH 3356 MOLECULAR BIOLOGY LABORATORY Fall 2011 LABORATORY MANUAL Coordinator: Luc Poitras Technicians: Christian Prud’homme…

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EXTRACTION OF PROTEIN The first step in the extraction of protein is liberation of protein from the cells that contain it and to get it in to solution. The method of choice…

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1. CURRICULUM AND INSTRUCTIONAL MATERIALS Summer Fellows 2011 Jeanette Mowery and Lisa Seidman 2. Overview,big picture Focus on three specific initiatives 3. CORE GOAL OF…

Documents Figure 4.3. Gel Filtration Chromatography. A mixture of proteins in a small volume is applied to a.....

Slide 1 Slide 2 Figure 4.3. Gel Filtration Chromatography. A mixture of proteins in a small volume is applied to a column filled with porous beads. Because large proteins…

Documents 1. 2 2 Protein Purification: From industrial enzymes to cancer therapy.

Slide 11 Slide 2 2 2 Protein Purification: From industrial enzymes to cancer therapy Slide 3 3 Protein Expression and Purification Series Instructors Jim DeKloe Solano Community…