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Expression of Foreign Proteins in E. Coli -A Practical approach Navin Khanna Overview of a practical approach • • • • • • • • • • • Gene isolation:…

Documents Restriction Map: 1 PstI PstI + CcoIV 700030007000 4000 PstI CcoI 7Kbp 1 cut 2 cuts 3Kbp 4Kbp.

Slide 1 Restriction Map: 1 PstI PstI + CcoIV 700030007000 4000 PstI CcoI 7Kbp 1 cut 2 cuts 3Kbp 4Kbp Slide 2 Restriction Map: 2 EcoRi EcoRI + BstEII 200100 800200900 700…

Documents Restriction Map 1 Construct a plasmid restriction map of the following digest. Include cuts and...

Slide 1 Restriction Map 1 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. EcoRI EcoRI + HindIII 7,0006,00010,000 3,0003,000…