KARAKTERISTIK DAN FUNGSI SEL KOMPETEN Sel kompeten memiliki kemampuan untuk berikatan, dan mengambil DNA eksogen. Sel kompeten merupakan sel yang telah siap untuk ditransformasi…

Expression of Foreign Proteins in E. Coli -A Practical approach Navin Khanna Overview of a practical approach • • • • • • • • • • • Gene isolation:…

Slide 1 Science, Technology, Engineering, and Math all rolled up into iGEM Ung.igem.org/High_School_Division Slide 2 Foundation Science for iGEM Synthetic Biology take molecular…

Slide 1 Bacterial Transformation Slide 2 Broad and Long Term Objective To characterize a single clone from an Emiliania huxleyi cDNA library using sequence analysis To characterize…

Slide 1 CHAPTER 4 DNA CLONING MISS NUR SHALENA SOFIAN Slide 2 INTRODUCTION Scientists in early years were able to isolate fragments of DNA in vitro and covalently joined…

Slide 1 Engineering the Subtilisin Gene into E. coli MoldBusters Oggie Golub Katy Hood Derek Stewart Casy Cory Josh Mauldin Slide 2 Proposal Project Goal –To engineer the…

Slide 1 Types of cloning vectors 1. plasmids (about 20kb) 2. Bacteriophage (bacterial viruses), 30-50kb inserts 3. Cosmids (35-50kb insert) 4. BACs Use fertility F plasmid…