Slide 1General Characteristics of Gas Detectors Slide 2 Signal Creation Charged particles traversing matter leaving excited atoms, electron or holes and ions behind. These…

Introduction to Fluorescence Techniques Fluorescent probes enable researchers to detect particular components of complex biomolecular assemblies, including live cells, with…

1 Fluorescence: • Absorption • Relaxation to ground vibrational level of S1. • Emission The lifetime of the excited state (~ 10-8 sec) makes fluorescence a valuable…

Slide 1 I - PALM Super-resolution Methods Slide 2 Detecting A Single Fluorescent Molecule? Size: ~ 1nm Absorption Cross-section: ~ 10 -16 cm 2 Quantum Yield: ~1 Absorbance…

Super-resolution Methods I - PALM Detecting A Single Fluorescent Molecule? Size: ~ 1nm Absorption Cross-section: ~ 10-16 cm2 Quantum Yield: ~1 Absorbance of 1 molecule =…

Epi-illumination is form of Kohler Illumination: Objective is also condenser Lamp or laser detector Detect at 90 degrees Split with dichroic mirror Greatly increases S/N…