Documents I - PALM Super-resolution Methods. Detecting A Single Fluorescent Molecule? Size: ~ 1nm Absorption.....

Slide 1 I - PALM Super-resolution Methods Slide 2 Detecting A Single Fluorescent Molecule? Size: ~ 1nm Absorption Cross-section: ~ 10 -16 cm 2 Quantum Yield: ~1 Absorbance…

Documents Super-resolution Methods

Super-resolution Methods I - PALM Detecting A Single Fluorescent Molecule? Size: ~ 1nm Absorption Cross-section: ~ 10-16 cm2 Quantum Yield: ~1 Absorbance of 1 molecule =…

Documents Epi-illumination is form of Kohler Illumination: Objective is also condenser Lamp or laser detector....

Epi-illumination is form of Kohler Illumination: Objective is also condenser Lamp or laser detector Detect at 90 degrees Split with dichroic mirror Greatly increases S/N…