Slide 1 PHAGE DNA Vector for cloning large DNA inserts La regione N-J (circa 19 kb) può essere rimossa o sostituita senza danneggiare il ciclo litico del fago. Slide 2 Replicazione…
Restriction Enzymes Graham Churchwell What are Restriction Enzymes? ► Also referred to as Restriction Endonucleases ► Enters and recognizes a certain sequence on a double…
Restriction Digestion Restriction Digest Today: Setup digest and incubate Before Thanksgiving: we will run the gel electrophoresis and analyze the results What are restriction…
Slide 1 Sequencing a genome and Basic Sequence Alignment Lecture 10 1Global Sequence Slide 2 Annotation of sequences Once the gene sequence’s have been determined then…
Slide 1 Restriction Enzyme Digestion & Southern Blotting of DNA Slide 2 Experiment Goals Digestion of DNA by restriction enzyme Analyze digested DNA by electrophoresis…
Slide 1 Overview of Protein Expression Expression systems are based on the insertion of a gene into a host cell for its translation and expression into protein. Introduction…
Slide 1 Chapter 13 Genetic Engineering Slide 2 Selective Breeding Where did all the breeds of dogs come from? ◦ Humans bred dogs to have certain traits, breeding dogs that…
Slide 1 Chapter 13 Genetic Engineering Selective Breeding- choosing what parents you want to produce offspring for the next generation. What do get when you cross a bull…