Slide 1BioRad pGLO: BioRad pGLO: Transform bacteria with a Jellyfish gene to make them glow Module based on a kit from Bio-Rad Laboratories, Inc. Adapted by Dan Murray from…

1.   2. pGLO™ Transformation and Purification ofGreen Fluorescent Protein (GFP) 3. Stan Hitomi Coordinator – Math & Science Principal – Alamo School San Ramon…

Slide 1PENNCREST HIGH SCHOOL KEYSTONE EXAM PREP Slide 2 PENNCREST HIGH SCHOOL KEYSTONE EXAM PREP 1. Which characteristic is shared by all prokaryotes and eukaryotes? a. ability…

Slide 1 Slide 2 pGLO™ Transformation and Purification of Green Fluorescent Protein (GFP) Slide 3 Stan Hitomi Coordinator – Math & Science San Ramon Valley Unified…

Slide 1 pGLO ™ & GFP Slide 2 Uses of GFP GFP is a visual marker Study of biological processes (example: synthesis of proteins) Localization and regulation of gene expression…

Slide 1 Pulse Electroporation System Onyx Medical Inc. Slide 2 Various TDD Technologies Various TDD Technologies  Electroporation - Using short electrical modulated pulses…

pGLO⢠& GFP Central Framework of Molecular Biology What is Transformation? Uptake of foreign DNA, often a circular plasmid What is a plasmid? A circular piece of autonomously…

PRESENTATION DOâS AND Muscle contains proteins of many sizes Protein kDa Function titin 3000 center myosin in sarcomere dystrophin 400 anchoring to plasma membrane filamin…

Transformation of E. coli with Green Fluorescent Protein (GFP) Central Framework of Molecular Biology Links to Real-world GFP is a visual marker Study of biological processes…

This Little Light of Mine: Transform bacteria with a Jellyfish gene to make them glow Aequorea victoria: Source of âglowing geneâ for this experiment Jellyfish Gene put…