Pulse Electroporation System Onyx Medical Inc.
Dec 24, 2015
Pulse Electroporation System
Onyx Medical Inc.
Various TDD Technologies Various TDD Technologies ElectroporationElectroporation - - Using short electrical modulated pulses of Using short electrical modulated pulses of
high voltage to create transient aqueous pores in the skin.high voltage to create transient aqueous pores in the skin. IontophoresisIontophoresis – Using low voltage electrical current to drive – Using low voltage electrical current to drive
charged drugs through the skin.charged drugs through the skin. SonophoresisSonophoresis – Using ultrasound to disrupt the stratum – Using ultrasound to disrupt the stratum
corneum. corneum. ThermalThermal energyenergy – Using heat to make the skin more permeable – Using heat to make the skin more permeable MagnetophoresisMagnetophoresis - has been investigated as a means to - has been investigated as a means to
increase drug flux across the skin.increase drug flux across the skin. Needle Free InjectionNeedle Free Injection - devices for “firing” molecules through - devices for “firing” molecules through
the skin at high velocitythe skin at high velocity
ElectroporationElectroporation
Non-invasiveNon-invasive Alternative to mesotherapyAlternative to mesotherapy Virtual mesotherapy or Virtual mesotherapy or
without needles without needles Painless procedurePainless procedure Introduction of drugsIntroduction of drugs alternating currentalternating current rearrange the structure in the rearrange the structure in the
cells of the outer layer of skincells of the outer layer of skin Acts as a virtual syringeActs as a virtual syringe
Changes direction (polarity) Changes direction (polarity) continuously slowlycontinuously slowly
Generating new poresGenerating new pores Micro and macromoleculesMicro and macromolecules 500,000 Dalton as collagen.500,000 Dalton as collagen. 800.000 Dalton as hyaluronic 800.000 Dalton as hyaluronic
acidacid
ElectroporationElectroporation
For electroporation we need:For electroporation we need: Membrane potential: 0.5-1.5 voltsMembrane potential: 0.5-1.5 volts Disrupt lipid layerDisrupt lipid layer Open biological channels (aquous)Open biological channels (aquous) These channels stay open several secondsThese channels stay open several seconds Change polarity constantlyChange polarity constantly
When the pulse ceases, the membrane returns to its When the pulse ceases, the membrane returns to its original structure, leaving retained product that has original structure, leaving retained product that has
penetrated into the interior of the cell in place. penetrated into the interior of the cell in place.
The application of an electrical pulse momentarily disrupts the cell membrane, allowing entry of
product.
Background
ElectroporationElectroporation
Electric doors called Electric doors called biological channelsbiological channels
Electric pulse changes Electric pulse changes membrane polaritymembrane polarity
By slowly changing the By slowly changing the polarization we do not polarization we do not cause electrolysiscause electrolysis
This disrupts lipid layerThis disrupts lipid layer
Open ChannelsOpen Channels
Intercellular CommunicationsIntercellular Communications
Cellular PenetrationCellular Penetration
ElectroporationElectroporation
This effect lasts for seconds, therefore allowing the introduction of This effect lasts for seconds, therefore allowing the introduction of substances.substances.
Hydrofilic and hydrophobic molecules penetrate at the rate of Hydrofilic and hydrophobic molecules penetrate at the rate of 1 g every 1 - 1.5 minutes1 g every 1 - 1.5 minutes..
Continuous formation and appearance of biological channelsContinuous formation and appearance of biological channels Dramatically increases skin permeabilityDramatically increases skin permeability..
ConclusionConclusion
Electroporation is a process that uses high-voltage current to Electroporation is a process that uses high-voltage current to make cell membranes permeable to allow the introduction of make cell membranes permeable to allow the introduction of new materials.new materials.
In a practical sense Electroporation is opening a door that In a practical sense Electroporation is opening a door that
allows us to insert “products” into the bodyallows us to insert “products” into the body..
MethodologyMethodology
Research documents 4 times Research documents 4 times better results when you do an better results when you do an exfoliation such as a peel or exfoliation such as a peel or micromicrodermabrasion micromicrodermabrasion before Electroporation.before Electroporation.
This eliminates dead cells This eliminates dead cells from the Keratinic layer.from the Keratinic layer.
This changes the skin This changes the skin potential and makes it easier potential and makes it easier to create biological channels.to create biological channels.
Comparison with MicrodermabrationComparison with Microdermabration
IndicationsIndications
Introduction of substances for:Introduction of substances for:– Skin treatmentSkin treatment– CelluliteCellulite– FlaccidityFlaccidity– WrinklesWrinkles– ObesityObesity– Collagen productionCollagen production
ContraindicacionesContraindicaciones
Pacemakers, heart valves Pacemakers, heart valves or other active implants. or other active implants.
EpilepsyEpilepsy Pregnant womenPregnant women Immediately after Botox Skin outbreaks or on Skin outbreaks or on
infected tissueinfected tissue Metal plates or other Metal plates or other
metal objectsmetal objects Ulcer or gastroenteritisUlcer or gastroenteritis
Wrinkles
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power:70% - 80%Time of application: 20 – 25 minutes
Amount of product:4 to 6 cc, divided into face, neck, and décolleté
Method:Circular motion, soft and slow until completely absorbed
Frequency of session:One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance
Decolette
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power: 70% - 80% Time of application : 15 – 20 minutes
Amount of product :4 cc
Method:Circular motion, soft and slow until completely absorbed
Frequency of session:One session a week during five to six weeks After one session every two week for one month A monthly session as maintenance
Body Firming
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power: 80% - 90%Time of application: 15 – 20 minutes
Amount of product: 5 to 6 cc (depending if we have to reduce or reaffirm)
Method:Circular motion, soft and slow until completely absorbed
Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance
Breast Firming
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion or gentle enzyme peel.
Select Protocol
Power: 80% - 90%Time of application: 15 – 20 minutes
Amount of product: 3 - 4 cc
Method:Circular motion, soft and slow until completely absorbed
Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance
Facial Rejuvenation
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power: 70% + Face, 30-40% LipsTime of application: 20 – 25 minutes
Amount of product: 5 to 6 cc, divided into face, neck, and chest (also hands)
Method:Circular motion, soft and slow until absorbed
Frequency of session:One session a week for three to four weeks
After one session every two week for one monthA monthly session as maintenance
Localized Obesity
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power: 85% - 95%Time of application: 15 – 20 minutes
Amount of product: 6cc - 10 cc
Method:Circular motion, soft and slow until absorbed
Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance
Cellulite
Instruction:Clean and dry skin the skin. Exfoliate by microdermabrasion. Optional: Use a micro roller after microderm.
Select Protocol
Power: 85% - 95%Time of application: 15 – 20 minutes
Amount of product: 6 - 10 cc
Method:Circular motion, soft and slow until absorbed
Frequency of session: One session a week during six to eight weeks After one session every two week for one month A monthly session as maintenance
ELECTROPORATIONELECTROPORATION
ONYX MEDICAL INC.ONYX MEDICAL INC.
1-800-333-57731-800-333-5773
WWW.ONYXMEDICAL.COMWWW.ONYXMEDICAL.COM