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CliniMACS Electroporator For use with CliniMACS Prodigy Matthew Cobb, Clinical Account Manager; Cell and Gene Therapy
11

AMC Miltenyi Electroporation MCobb - HOME - amc

Jan 28, 2022

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Page 1: AMC Miltenyi Electroporation MCobb - HOME - amc

CliniMACS ElectroporatorFor use with CliniMACS Prodigy

Matthew Cobb, Clinical Account Manager; Cell and Gene Therapy

Page 2: AMC Miltenyi Electroporation MCobb - HOME - amc

Recap – Prodigy Validated Applications

BM-133 EnrichmentBM-133 Enrichment

LP-34 EnrichmentLP-34 Enrichment

LP-TCRab/19 DepletionLP-TCRab/19 Depletion

CCS-IFN EnrichmentCCS-IFN Enrichment

LP-14 Enrichment LP-14 Enrichment

MoDC GenerationMoDC Generation

CAR T processCAR T process

Matthew Cobb AMC Bristol

Page 3: AMC Miltenyi Electroporation MCobb - HOME - amc

Electroporation - Applications

Matthew Cobb AMC Bristol

Page 4: AMC Miltenyi Electroporation MCobb - HOME - amc

Electroporation - Module Addition

Electroporation cuvette(4 mm or 2 mm)

Bag for cells

Bag for Electroporation

material

Cells provided from

external source orfrom CliniMACS Prodigy

Two connections to

Prodigy Tubing Sets

Matthew Cobb AMC Bristol

Page 5: AMC Miltenyi Electroporation MCobb - HOME - amc

Validation - Test Cuvette Adapter (TCA)

� Included in delivery

of the CliniMACS Electroporator

� Adapter to enable manual cuvettes to be used

on Prodigy Electroporator

� Used for initial tests and demonstrations

e.g. pulse optimisation, electroporation efficiency,

viability etc.

� Easy upscaling for testing and transfer of

electroporation parameters on the CliniMACS

Electroporator

� Transfer of optimised conditions to tubing sets

Matthew Cobb AMC Bristol

Page 6: AMC Miltenyi Electroporation MCobb - HOME - amc

CliniMACS Prodigy and Electroporator - Tubing Set(s)

� EP-2 tubing set (2mm)

� EP-4 tubing set (4mm)

� Electroporation cuvette

(2 mm / 4 mm

electrode distance)

� 2 Cell bags

� 1 material bag

� Combination of Prodigy

and Electroporator tubing

sets

Matthew Cobb AMC Bristol

Page 7: AMC Miltenyi Electroporation MCobb - HOME - amc

CliniMACS Electroporator – Electroporation chamber

� CliniMACS Prodigy Controlled

� Closed system

� Automated, cyclic electroporation of 15-50ml

� Square wave pulse (single or double)

� Variable protocols and pulsing parameters (500V-1000V)

� Wider facility of cell product manufacturing

� A broader platform for cell manufacturing

Matthew Cobb AMC Bristol

Page 8: AMC Miltenyi Electroporation MCobb - HOME - amc

Finding the right balance for parameter input

Matthew Cobb AMC Bristol

Page 9: AMC Miltenyi Electroporation MCobb - HOME - amc

Electroporation - Process Indication by colour

Matthew Cobb AMC Bristol

Page 10: AMC Miltenyi Electroporation MCobb - HOME - amc

Requirements and Solutions

Just like the Prodigy, the electroporator can

be used from process development to clinical production.

Matthew Cobb AMC Bristol

Customer transfection needs CliniMACS Electroporator solutions:

Viability > transfection efficiency TCA for electroporation optimisation

Large amounts of cells to be

electroporated

Automated loading for large volumes

Can be electroporated in a sterile,

closed system

Sterile tubing sets

Cell expansion after electroporation Cells directed to CCU

Enriched population Process on Prodigy

Page 11: AMC Miltenyi Electroporation MCobb - HOME - amc

Contact Details and Questions

• Matthew Cobb

• Clinical Account Manager (UK North)

[email protected]

• Michael Schenk

• Clinical Applications Specialist

[email protected]

• Antonio Di Rocco

• Clinical Applications Specialist

[email protected]

Matthew Cobb AMC Bristol