Technique of In-Vitro PropagationTechnique of In-Vitro Propagation
Dr. Sukendah, MSc
Clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained inculture vessels maintained under controlled conditions of light and temperature =MICROPROPAGATION
Explant
Cell, tissue or organ of a plant that is used to start in vitro cultures.
Explant
Cell, tissue or organ of a plant that is used to start in vitro cultures.
In vitro propagation
Tissue culture propagation
Micropropagation
Advantages
Micropropagation
Advantages
• From one to many propagules rapidly
• Multiplication in controlled lab conditions
• Continuous propagation year round
• Potential for disease-free propagules
Conventional Propagation AdvantagesConventional Propagation Advantages
• Equipment costs minimal
• Experience can substitute for technical expertise
• Costs per unit are minimal
• Potential for disease-free propagules
• Specialized techniques for growth control (grafting onto dwarfing rootstocks)
Micropropagation
Limitations
Micropropagation
Limitations
• Equipment/facility intensive operation
• Technical expertise in management positions
• Protocols not optimized for all species
• Liners may not fit industry standard
• Propagules may be too expensive
Micropropagation
Applications
Micropropagation
Applications
• Rapid build up of stock of new, superior variety
• Elimination of endophytic disease
• Better suited to morphology/growth characteristics (few offshoots/sprouts; date palms, ferns, nandinas)
• Propagules have enhanced growth features (multibranched character; Ficus, Syngonium)
MACROPROPAGATIONMICROPROPAGATION
Small propaguleAseptic conditionsControlled environmentHeterotrophic growthRapid multiplicationGreater initial costs
Larger propaguleNon-aseptic conditionsLess environmental controlPhotoautotrophic growthSlower multiplicationNominal costs
Perbaikkan formulasi media yang penting adalah pengembangan unsur makro yang universal, untuk mendukung pertumbuahan jaringan tumbuhan. Dalam media perlu ditambahkan ammonium dengan meningkatkan konsentrasi NO3- dan K +.
Media Knop Media White Media Knudson dan media Vacin and Went Media Murashige & Skoog (media MS) Media Gamborg B5 (media B5) Media Schenk & Hildebrant (media SH) Media WPM (Woody Plant Medium)
Thank You
