Technique of In-Vitro Propagation Dr. Sukendah, MSc.

Technique of In-Vitro PropagationTechnique of In-Vitro Propagation

Dr. Sukendah, MSc

Clonal in vitro propagation of plants from cells, tissues or organs cultured aseptically on defined media contained inculture vessels maintained under controlled conditions of light and temperature =MICROPROPAGATION

Explant

Cell, tissue or organ of a plant that is used to start in vitro cultures.

Explant

Cell, tissue or organ of a plant that is used to start in vitro cultures.

In vitro propagation

Tissue culture propagation

Micropropagation

Advantages

Micropropagation

Advantages

• From one to many propagules rapidly

• Multiplication in controlled lab conditions

• Continuous propagation year round

• Potential for disease-free propagules

Conventional Propagation AdvantagesConventional Propagation Advantages

• Equipment costs minimal

• Experience can substitute for technical expertise

• Costs per unit are minimal

• Potential for disease-free propagules

• Specialized techniques for growth control (grafting onto dwarfing rootstocks)

Micropropagation

Limitations

Micropropagation

Limitations

• Equipment/facility intensive operation

• Technical expertise in management positions

• Protocols not optimized for all species

• Liners may not fit industry standard

• Propagules may be too expensive

Micropropagation

Applications

Micropropagation

Applications

• Rapid build up of stock of new, superior variety

• Elimination of endophytic disease

• Better suited to morphology/growth characteristics (few offshoots/sprouts; date palms, ferns, nandinas)

• Propagules have enhanced growth features (multibranched character; Ficus, Syngonium)

MACROPROPAGATIONMICROPROPAGATION

Small propaguleAseptic conditionsControlled environmentHeterotrophic growthRapid multiplicationGreater initial costs

Larger propaguleNon-aseptic conditionsLess environmental controlPhotoautotrophic growthSlower multiplicationNominal costs

Perbaikkan formulasi media yang penting adalah pengembangan unsur makro yang universal, untuk mendukung pertumbuahan jaringan tumbuhan. Dalam media perlu ditambahkan ammonium dengan meningkatkan konsentrasi NO3- dan K +.

Media Knop Media White Media Knudson dan media Vacin and Went Media Murashige & Skoog (media MS) Media Gamborg B5 (media B5) Media Schenk & Hildebrant (media SH) Media WPM (Woody Plant Medium)

Thank You