Supplementary Figure S2
DMSO
1nM
10nM
100n
M
Rapamycin (24hr)
0
5
10
15
20p-AKT p-Src
DMSO
1 nM
10 n
M
100
nM
Rapamycin (3hr)
0
1
2
3
4
5
6p-AKT p-Src
phos
pho
: tot
al ra
tio
p-AKT
p-Src
p-S6
t-AKT
t-Src
t-S6
β-act
DMSO
1nM
10nM
100n
M
Rapamycin (24hr)
0
1
2
3
4p-AKT p-Src
MCF7 BT474 MDA-MB-231
Rapamycin induction of p-AKT occurs within 1 hour and is not due to increased levels of p-Src.
t-Src
p-AKT
t-AKT
MCF7 BT474 MDA-MB-231
p-Src
0hr 0.5hr 1hr 5hr 24hrRapamycin (10nM)
05
10152025 p-AKT p-Src
phos
pho:
tota
l rati
o
0hr
0.5h
r
1hr
5hr
24hr
Rapamycin (10nM)
02468
10 p-AKT p-Src
0hr 0.5hr 1hr 5hr 24hrRapamycin (100nM)
0
10
20
30
40 p-AKT p-Src
A
B