Supplementary Figure S2 DMSO 1nM 10nM 100nM Rapamycin (24hr) 0 5 10 15 20 p-AKT p-Src DMSO 1 nM 10 nM 100 nM Rapamycin (3hr) 0 1 2 3 4 5 6 p-AKT p-Src phospho : total ratio p-AKT p-Src p-S6 t-AKT t-Src t-S6 β-act DMSO 1nM 10nM 100nM Rapamycin (24hr) 0 1 2 3 4 p-AKT p-Src MCF7 BT474 MDA-MB-231 Rapamycin induction of p-AKT occurs within 1 hour and is not due to increased levels of p-Src. t-Src p-AKT t-AKT MCF7 BT474 MDA-MB-231 p-Src 0hr 0.5hr 1hr 5hr 24hr Rapamycin (10nM) 0 5 10 15 20 25 phospho:total ratio 0hr 0.5hr 1hr 5hr 24hr Rapamycin (10nM) 0 2 4 6 8 10 p-AKT p-Src 0hr 0.5hr 1hr 5hr 24hr Rapamycin (100nM) 0 10 20 30 40 p-AKT p-Src A B