Problem StatementProblem Statement
GOAL:GOAL: To modify the needle used during a To modify the needle used during a Fine Needle Aspiration (FNA) procedure. Fine Needle Aspiration (FNA) procedure. The modification should not drastically The modification should not drastically change the FNA procedure and still change the FNA procedure and still increase the amount of tissue removed for increase the amount of tissue removed for cytological testing. It should also minimize cytological testing. It should also minimize the time (by collecting an adequate sample the time (by collecting an adequate sample size on the first attempt) and discomfort size on the first attempt) and discomfort caused to the patient during the procedurecaused to the patient during the procedure. .
What is FNA?What is FNA?
Technique used to Technique used to extract cells; no extract cells; no incision neededincision neededSeveral insertions are Several insertions are usually required to usually required to ensure an adequate ensure an adequate sample sizesample sizeSamples are sent to Samples are sent to pathologist to be pathologist to be analyzedanalyzed
National Breast Cancer Center, http://www.NBCC.org
Fine Needle Aspiration (FNA)
A.D.A.M., http://www.adam.com
Product Design SpecificationsProduct Design Specifications
Removes adequate number of cells for Removes adequate number of cells for diagnosis.diagnosis.
Should not cause more discomfort than Should not cause more discomfort than current FNA procedure.current FNA procedure.
Constrained to standard 20-25-gauge Constrained to standard 20-25-gauge needle, client prefers 23-gauge.needle, client prefers 23-gauge.
Current PrototypeCurrent Prototype
Pre-manufactured drill bitPre-manufactured drill bit
6” length6” length
Fits in 23 gauge needleFits in 23 gauge needle
Tissues TestedTissues Tested
Fixed cat tissuesFixed cat tissues
– MammaryMammary
– LymphLymph
– FatFat
Testing ProceduresTesting Procedures
Old techniqueOld technique– No insert usedNo insert used– Performed two separate trials on all three Performed two separate trials on all three
tissuestissues
New techniqueNew technique– Drill bit insert usedDrill bit insert used– Performed two separate trials on all three Performed two separate trials on all three
tissuestissues
Observed in saline bath under microscopeObserved in saline bath under microscope
Sample QuantificationSample Quantification
Old techniqueOld technique
– Resulted in either a mass of cells or a Resulted in either a mass of cells or a minimal quantity of cells minimal quantity of cells
New techniqueNew technique
– More dispersed cell sampleMore dispersed cell sample
– Greater number of cells on averageGreater number of cells on average
Future WorkFuture Work
Further prototype developmentFurther prototype development
Research into appropriate materials to be Research into appropriate materials to be used (stainless steel, Co-Cr, Ti)used (stainless steel, Co-Cr, Ti)
Develop protocol for quantifying testingDevelop protocol for quantifying testing
Test on freshly removed tissueTest on freshly removed tissue
In vivoIn vivo testing of animal tumors testing of animal tumors
Cytological evaluationCytological evaluation
Drill Bit Size ComparisonDrill Bit Size Comparison
Tissue Sample- MammaryTissue Sample- Mammary
Tissue Sample- LymphTissue Sample- Lymph
Tissue Sample- FatTissue Sample- Fat
Old Technique- MammaryOld Technique- Mammary
Old Technique- LymphOld Technique- Lymph
Old Technique- FatOld Technique- Fat
New Technique- MammaryNew Technique- Mammary
New Technique- LymphNew Technique- Lymph
New Technique- FatNew Technique- Fat