DNAmicroarrayPavlo Tertychnyi6March2017
Presentationoutline
1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults
Presentationoutline
1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults
WhatisDNAmicroarray
WhatisDNAmicroarray
Presentationoutline
1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults
Medicalfield
Helpresearcherslearnmoreaboutdiseases:1. Whatcausesdiseases2. Howtoidentifydiseases3. Howtotreatthem
Multiplesclerosis
Foodproduction
Foodproduction
Please,don’teatme…
Presentationoutline
1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults
Howdoesitwork
Howdoesitwork
Howdoesitwork
Example
Presentationoutline
1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults
Analyzingofresults
Analyzingofresults
Analyzingofresults
Analyzingofresults
DNAmicroarraydatabases
Summary
1. Usedtoidentifyspecificgenesandtheactivityofgenes.2. Usedtodeterminehowourgenesareaffectedbyenvironmental
factors,drugsanddrugdosages.3. Wideusedinmedicinefield4. Identifyspecificcharacteristicsinhumanaswellasotherspecies.5. Foodproduction
Coolvisualization
https://www.youtube.com/watch?v=VNsThMNjKhM
Acknowledgement1. http://scme-
nm.org/index.php?option=com_docman&task=cat_view&gid=97&Itemid=225
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011503/pdf/nihms-563981.pdf
3. http://www.ub.edu/stat/docencia/bioinformatica/microarrays/ADM/slides/A_Tutorial_Review_of_Microarray_data_Analysis_17-06-08.pdf
4.http://www.ebi.ac.uk/arrayexpress/5.https://www.ncbi.nlm.nih.gov/geo/6.http://www.hhmi.org/biointeractive/how-analyze-dna-microarray-data
Homework
1.WhatisDNAmicroarray?
a)Specificdatatypeb)Microchipc)SubsequenceofaDNAd)Giftofthegods
Homework
2.Describeatleast3applicationofDNAmicroarray(Imentionedtwoapplicationsduringthepresentation,souseyourimaginationorjustgoogletofindmore).3.DescribetheprocessofpreparationofamaterialbeforepouringitonaDNAmicroarray.
Homework
4.Jointhenextstatements:
a)Therearegenesofahealthycellmainly.b)Therearegenesofasickcellmainly.c)Therearegenesofbothhealthyandsickcells.
1)RedcolorofDNAmicroarrayunit.2)GreencolorofDNAmicroarrayunit.3)YellowcolorofDNAmicroarrayunit.
Thanks!
