30/03/1311تاريخ پذيرش 30/11/1311تاريخ دريافت
a-TNF1B-ILDRG
STZ DRG
TNF-aIL-1BDRGp=0.001
TNF-a
p=0.001
TNF-aDRG
TNF-aIL-1B
Email: [email protected]
Dorsal Root Ganglia
Streptozotocin
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C
DRG
TNF-αIL-
1B
DRG
Zhu
a-TNFDRG
IL-1BIL-1B
ROS
(4-allyl-2-metoxoxhenhe
Spinal cord
ROS
TNF-aIL1B
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DRGTNF-aIL-1B
kgmg /
DMSO
ml
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H&E
ZEISS
Image J(Image
J Software, NIH)
Real Time PCR
RNA
RNA
DEPC
RNA
Vortex
RNA
cDNA
Fermentas, USAcDNA
TNF-aIL-1B Real
time-PCR
(GAPDH)
-ΔΔCT2
TNF-aIL-1BGAPDH
NCBI
5ˈ-3ˈ
NM_012675.3 F5' GAGATGTGGAAATGGCAGAGGA 3'
R5' GAGAAGATGATGTGAGTGTGAGG 3'
TNF-a
NM_031512.2 F5' TGTGACTGGTGGGATGATGA 3'
R5' GTTCTGTCTATTGAGGTGGAGA3'
IL-1B
XM_017593963.1 F 5' CAT ACT CAG CAC CAG CAT CAC C 3'
R5' AAG TTC AAC GGC ACA GTC AAG G 3'
GAPDH
ANOVA
SPSS
≤P
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DRG
H&E
DRGp=0.001
p=0.001
p=0.01p=0.001
. اندشدهف استاندارد نشان داده انحرا ±ن يانگيم صورتبه هاداده ب(.) يونيگانگل يهاسلولو تعداد ()الف DRGک يستولوژيرات هييتغ (:1)شکل
نشان دهنده ** و با گروه کنترل سهيمقادر (p≤0.05) دار ي* نشان دهنده تفاوت معنموش در هر گروه. 21 يمده از بررسآ دستبهنتابج
است. يابتيمدل دسه با گروه يدر مقا (p≤0.05) دار يتفاوت معن
DRG
mRNA TNF-a
p=0.001
TNF-ap=0.001
05
101520253035404550
*
*
*
**
**
الف
ب
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mRNA IL-1B
DRG
p=0.001
mRNA IL-1BDRG
TNF-aIL-1B
p≤0.05
p≤0.05
0
0.0002
0.0004
0.0006
0.0008
0.001
0.0012
0.0014
0.0016
0.0018
دهش
ل ما
ر نن
ژن
یاب
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0
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0.002
0.003
0.004
0.005
0.006
0.007
0.008
دهش
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الف
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TNF-a
IL-1BSTZ
IL1-α)IL1-β
IL-2Il-4IL-6
IL-10IFN-γTGF-
β)TNF-αDRG
TNF-α
TNF-
aIL-1B
DRG
TNF-αIL1-β
DRG
TNF-a
IL-B
IL-6
IL-10 IL-
1RAIL-6
IL-6
TNF-α
IL-6DRG
PIL-6TNF-
αNR1IL-1βP
TNF-αIL1-β
DRG
DRG
TNF-
aDRG
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IL-1B
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Studies in Medical Sciences, Vol. 31(5), August 2020
Original Article
THE EFFECT OF 8 WEEKS OF AEROBIC EXERCISE WITH NANO
EUGENOL SUPPLEMENTATION ON INFLAMMATORY FACTORS
OF TNF-A AND IL-1B AND HISTOLOGICAL CHANGES OF DORSAL
ROOT GANGLION IN DIABETIC RATS
Abbas Shareghi boroujeni 1, khosro Jalali Dehkordi *2, Gholamreza sharifi 3,
Farzaneh Taghian 4, Zohreh Mazaheri5
Received: 19 February, 2020; Accepted: 19 June, 2020
Abstract Background & Aims: The aim of this study was to investigate the effect of 8 weeks of aerobic exercise
with Nano eugenol supplementation on inflammatory factors of TNF-a and IL-1B and histological
changes of dorsal root ganglion in diabetic rats.
Materials & Methods: 25 Wistar 8-week-old male rats were divided into 5 groups: Normal control
group, Diabetic control group (Model), Diabetic + exercise group (Model + Exe), Diabetic group + Nano
vaginal (Model + Nano), and Diabetic + exercise training + nano eugenol (Model + Exe + Nano). The
diabetes model was induced by peritoneal injection of streptozotocin to the respective groups. The
eugenol supplement was also gavaged to the supplement groups. Exercise groups also exercised for 8
weeks, 5 days a week (30 m / min).
Results: Induction of diabetes using STZ led to destruction of the tissue and cell alignment in the DRG
region. Gene changes also showed that TNF-a and IL-1B inflammatory factors showed a significant
increase in the DRG region in the diabetic group compared to the control group (p = 0.001 for both
variables). The study of therapeutic modalities also showed that only the diabetic + exercise +
nanougenol group showed a significant decrease in TNF-a compared to the diabetic group (p = 0.001).
Conclusion: According to the results of the present study, it seems that the use of nanougenol
supplementation along with exercise training may be effective in controlling the neurological damage
of diabetes by negatively regulating the inflammatory factor TNF-a, especially in the DRG region.
Keywords: Aerobic Exercise, Nano eugenol, TNF-a, IL-1B, Diabetes
Address: Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Tel: +989131854997
Email: [email protected]
SOURCE: STUD MED SCI 2020: 31(5): 409 ISSN: 2717-008X
PhD student, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch, Islamic
Azad University, Isfahan, Iran
Assistant Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,
Islamic Azad University, Isfahan, Iran (Corresponding Author)
Associate Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,
Islamic Azad University, Isfahan, Iran
Associate Professor, Department of Sport Physiology, School of Sport Sciences, Isfahan (Khorasgan) Branch,
Islamic Azad University, Isfahan, Iran
Histogenic Laboratory, Tehran, Iran
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