1 23 EcoHealth Conservation Medicine: Human Health:Ecosystem Sustainability Official journal of International Association for Ecology and Health ISSN 1612-9202 EcoHealth DOI 10.1007/s10393-011-0725-7 Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand Kim Blasdell, Jean François Cosson, Yannick Chaval, Vincent Herbreteau, Bounneuang Douangboupha, Sathaporn Jittapalapong, Ake Lundqvist, et al.
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Rodent-Borne Hantaviruses in Cambodia, Lao PDR, and Thailand
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EcoHealthConservation Medicine: HumanHealth:Ecosystem Sustainability Officialjournal of International Association forEcology and Health ISSN 1612-9202 EcoHealthDOI 10.1007/s10393-011-0725-7
Rodent-Borne Hantaviruses in Cambodia,Lao PDR, and Thailand
Kim Blasdell, Jean François Cosson,Yannick Chaval, Vincent Herbreteau,Bounneuang Douangboupha, SathapornJittapalapong, Ake Lundqvist, et al.
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Rodent-Borne Hantaviruses in Cambodia, Lao PDR,and Thailand
Kim Blasdell,1,2 Jean Francois Cosson,3 Yannick Chaval,4 Vincent Herbreteau,5
1Virology Unit, Institut Pasteur du Cambodge, 5 Monivong Boulevard, BP 983, Phnom Penh, Cambodia2Institut des Sciences de l’Evolution, CNRS, IRD, Universite Montpellier 2, CC065, 34095 Montpellier Cedex 05, France3INRA, UMR CBGP (INRA/IRD/Cirad/Montpellier SupAgro), Campus International de Baillarguet, CS 30016, 34988 Montferrier sur Lez, France4Centre de Biologie et de Gestion et des Populations (CBGP), International de Baillarguet, CS 30016, 34988 Montferrier sur lez, France5CIRAD, UR AGIRs (Animal et Gestion Integree des Risques), Campus International de Baillarguet, Montpellier, France6National Agricultural Research Centre, National Agricultural and Forestry Research Institute, Vientiane, Lao PDR7Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand8Swedish Institute for Infectious Disease Control, Stockholm, Sweden9Origine, Structure et Evolution de la Biodiversite, Museum National d’Histoire Naturelle, Paris, France
Abstract: In order to evaluate the circulation of hantaviruses present in southeast Asia, a large scale survey of
small mammal species was carried out at seven main sites in the region (Cambodia, Lao People’s Democratic
Republic, and Thailand). Small scale opportunistic trapping was also performed at an eighth site (Cambodia).
Using a standard IFA test, IgG antibodies reacting to Hantaan virus antigens were detected at six sites.
Antibody prevalence at each site varied from 0 to 5.6% with antibodies detected in several rodent species
(Bandicota indica, B. savilei, Maxomys surifer, Mus caroli, M. cookii, Rattus exulans, R. nitidius, R. norvegicus,
and R. tanezumi). When site seroprevalence was compared with site species richness, seropositive animals were
found more frequently at sites with lower species richness. In order to confirm which hantavirus species were
present, a subset of samples was also subjected to RT-PCR. Hantaviral RNA was detected at a single site from
each country. Sequencing confirmed the presence of two hantavirus species, Thailand and Seoul viruses,
including one sample (from Lao PDR) representing a highly divergent strain of Seoul virus. This is the first
molecular evidence of hantavirus in Lao PDR and the first reported L segment sequence data for Thailand
virus.
Keywords: hantavirus, southeast Asia, rodents, serology, phylogeny, seoul virus variant
INTRODUCTION AND PURPOSE
Hantaviruses are single-stranded, tri-segmented, negative
sense RNA viruses belonging to the family Bunyaviridae.
Electronic supplementary material: The online version of this article
(doi:10.1007/s10393-011-0725-7) contains supplementary material, which is available
region where species richness is changing rapidly in many
areas.
ACKNOWLEDGMENTS
The authors wish to thank Mr. Hul Vibol and Mr. Y
Bunthin for their technical assistance in both the laboratory
and the field and to Mr. Kim Aun for his assistance in the
field. Also to Maria Walhstrom for kindly preparing the IFA
slides used in this study. The CERoPath project (Commu-
nity ecology of rodents and their pathogens in South East
Asia: effects of biodiversity changes and implications in
health ecology/ANR 07 BDIV 012) is funded by the Agence
Nationale de la Recherche (ANR, France).
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