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Ngs

Feb 08, 2017

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DNANext Generation DNA Sequencing Metod4

5 : : : 1394

5

(DNA sequencing) DNA 1970 . Sanger Maxam Gilbert & 1977 . Sanger . 1990 2003 .

6

Applied Biosystems (Capillary Electrophoresis ) CE . AB370 1987 AB3730xl 1998 .

7NGS

2004 . Next Generarion Sequencing (NGS) . 10 NGS .

8NGS

. DNA (ancient DNA) .9NGS

Resequencing)) NGS SNP ((Insertion / deletion . 2005 454 .

10NGS

. DNA . .

11 NGS

. NGS . NGS .12NGS

NGS :

13 NGS

2004 Roche/454FLX Pyrosequencer . (Pyrosequencing) Life Sciences Roche . DNA . . 14 Roche / 454

DNA ( PCR) . DNA . . (Luciferase Enzyme) DNA . Roche DNA 1000 .

15 Roch / 454

16

DNA ((A (B ) (C) 454

17Roche GS-Junior

18Roche GS-FLX

19The Roche-454 GS-FLX Pyrosequencer

Solexa Genome Analyzer Illumina Illumina / Solexa . 2010 Illumina Hiseq . .

20 Illumina / Solexa

(Reversible Dye-Terminators) . DNA (Bridge Amplification) . (RT-bases) DNA . . DNA ' 3 .

21 Illumina / Solexa

22.Illumina/Solexa Genome AnalyzerTM

23

Illumina S-L 1600

24Illumina MiSeq

25Illumina HiSeq

26illumina_nextseq500

SOLiD (Sequencing- by-ligation) (Applied Biosystems - ABI) . 1 2 . Gb Plus 3 SOLiD Gb .

27 ABI / SOLiD

DNA Aqueous droplets within an oil phase )) (Emulsion PCR). DNA . . . ligase .

28 ABI / SOLiD

29

DNA ABI / SOLiD

30

5500 SOLiD System

. :31

Pacific Bioscience (Single Molecular Real Time) SMRT. DNA . SMRT DNA .32 SMRT

Polony 2005 . . ABI () .33 Polony

Massively Parallel Signature Sequencing - MPSS 1992 . . . 2004 Selectra . .34 MPSS

Next-Next-Generation Sequencing" .

NGS) ) DNA PCR . bp .35 NNGS

Helicos Bioscience The Helicos Heliscope .

. 55 .36 Helicos Heliscope

37

Helicos Heliscope

Ion Torrent Machine Ion Personal Genome (Ion PGM) . DNA pH pH .38 Ion PGM

39Ion Torrent

Refrence40

41# Advanced sequencing technologies and their wider impact in microbiologyNeil HallThe Journal of Experimental Biology 209, 1518-1525

# Applications of next-generation sequencing technologies in functional genomics Olena Morozova, Marco A. MarraGenomics - Volume 92, Issue 5, November 2008, Pages 255264

42# A tale of three next generation sequencing Michael A Quail , Miriam Smith, Paul Coupland, Thomas D Otto, Simon R Harris, Thomas R Connor, Anna Bertoni, Harold P Swerdlow and Yong GuBMC Genomics 2012, 13:341

# Buyers Guide: Next-Generation Sequencing SystemsThis buyers resource is presented to you by Illumina Inc. # Next-generation DNA sequencing - Jay Shendure & Hanlee Ji, naturebiotechnology / volume 26. naumber 10. OCTOBER 2008

43# Sequencing Methods ReviewA review of publications featuring Illumina TechnologyPub No. 073-2014-001 Current as of 29 May 2014

# The advantages of SMRT sequencingRichard J Roberts,Mauricio OCarneiroandMichael C Schatz - Genome Biology2013,14:405

44# 6th Next Generation Sequencing Conference12th and 13th August 2014Dunedin Art GalleryThe Octagon, Dunedin, New Zealand

# www.illumina.com/

# www.ngsleaders.org/

# www.thermofisher.com/

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