NGS Technology
Dec 16, 2021
Creative Biolabs is a leading customer service provider in the field of next-generation sequencing (NGS). With years of research and development experience, our seasoned scientists have established a pioneer SuPrecision™ platform to offer accurate and high-throughput sequencing services to meet every unique requirement of our clients.
https://www.creative-biolabs.com/suprecision/
https://www.creative-biolabs.com/suprecision/
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Creative Biolabs is a leading life science company serving basic research. for any products and services, please visit: https://www.creative-biolabs.com/
Transcript
NGS technology-Creative BiolabsNext-generation sequencing (NGS) technologies have progressive advantages in terms of cost-effectiveness, unprecedented sequencing speed, high resolution and accuracy in genomic analy- ses, thus are playing an increasing important role in fields of oncology and immu- nology.
The sequencing principles of common sequencing instru- ments on the market: (1) first, construct a DNA template library. Obtain DNA library fragments (tens to hundreds of bases in length) by randomly breaking genomic DNA, or construct paired-end fragments that control the distance distribution. Adapter sequences were ligated to both ends of the DNA fragment and then denatured to obtain a single-stranded template library and immobi- lized on a solid surface. (2) Second, amplify the clones, which performed by one of several methods, such as bridge PCR and emulsion PCR. (3) Then, forming DNA clusters or amplifying micro- spheres of DNA cluster arrays on a chip, performing a series of cyclic reaction operations using a polymerase\ or ligase, and monitoring the optical events generated in each cycle biochemical reaction by a microscopic detection syst- em. Time series analysis of the resulting array images is performed to obtain sequenc- es of DNA fragments. These fragments are then assem- bled into longer contigs according to certain computer algorithms.
Creative Biolabs uses the advanced SuPrecision™ pl- atform to support researchers all over the world with their sequencing needs for cancer.
NEXT GENERATION SEQUENCING SERVICE
© 2007 - 2019 Creative-Biolabs All Rights Reserved Email: [email protected] Tel: 1-631-381-2994
WHAT WE DO: Whole Genome Sequencing (WGS) Service for Cancer Whole Exome Sequencing (WES) Service for Cancer Targeted Sequencing Service for Cancer
Whole Transcriptome Sequencing (WTS) Service for Cancer Immune Repertoire Sequencing (Rep-seq) Service for Cancer Bioinformatics Analysis Service
Cyclic Array Sequencing3
C G T
5'
5'
3'
The sequencing principles of common sequencing instru- ments on the market: (1) first, construct a DNA template library. Obtain DNA library fragments (tens to hundreds of bases in length) by randomly breaking genomic DNA, or construct paired-end fragments that control the distance distribution. Adapter sequences were ligated to both ends of the DNA fragment and then denatured to obtain a single-stranded template library and immobi- lized on a solid surface. (2) Second, amplify the clones, which performed by one of several methods, such as bridge PCR and emulsion PCR. (3) Then, forming DNA clusters or amplifying micro- spheres of DNA cluster arrays on a chip, performing a series of cyclic reaction operations using a polymerase\ or ligase, and monitoring the optical events generated in each cycle biochemical reaction by a microscopic detection syst- em. Time series analysis of the resulting array images is performed to obtain sequenc- es of DNA fragments. These fragments are then assem- bled into longer contigs according to certain computer algorithms.
Creative Biolabs uses the advanced SuPrecision™ pl- atform to support researchers all over the world with their sequencing needs for cancer.
NEXT GENERATION SEQUENCING SERVICE
© 2007 - 2019 Creative-Biolabs All Rights Reserved Email: [email protected] Tel: 1-631-381-2994
WHAT WE DO: Whole Genome Sequencing (WGS) Service for Cancer Whole Exome Sequencing (WES) Service for Cancer Targeted Sequencing Service for Cancer
Whole Transcriptome Sequencing (WTS) Service for Cancer Immune Repertoire Sequencing (Rep-seq) Service for Cancer Bioinformatics Analysis Service
Cyclic Array Sequencing3
C G T
5'
5'
3'
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