Lecture 15: DNA Organization, Replication and Repair Reading Assignment: Chapter 38, pgs. 412-434; Harper’s Biochemistry (25 th edition). Objective: To understand the structures and proteins involved in DNA replication. I. Cellular DNA exists as chromatin A. Nucleosomes 1. Histones II. General characteristics of DNA replication A. Semi-conservative B. Starts at specific sites C. Bidirectional III. General characteristics of DNA polymerases A. Use DNA as a template B. Replicate DNA with high fidelity C. Catalyze the formation of phosphodiester bridge IV. Characteristics and function of procaryotic DNA polymerases V. Characteristics and function of eucaryotic DNA polymerases