LAB-SCALE TECHNOLOGIES FOR THE CULTIVATION AND BIOREFINERY OF MICROALGAE SPEAKER: DAVIDE DE FRANCISCI TECHNICAL UNIVERSITY OF DENMARK This project has received funding from the European Union’s Seventh Programme for research, technological development and demonstration under grant agreement No 280756.
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LAB-SCALE TECHNOLOGIES FOR THE CULTIVATION AND BIOREFINERY OF MICROALGAE
SPEAKER: DAVIDE DE FRANCISCI TECHNICAL UNIVERSITY OF DENMARK
This project has received funding from the European Union’s Seventh Programme for research, technological development and demonstration under grant agreement No 280756.
E4Water Final Conference, Brussels, April 19-20, 2016 17
NOVOZYMES WASTEWATER
C. protothecoides
S. acutus
H. pluvialis
C. vulgaris
Unidentified diatom
The best candidate resulted to be Chlorella spp. S. obliquus and H. pluvialis also shows good growth on 100% WW.
E4Water Final Conference, Brussels, April 19-20, 2016 18
REACTOR CONFIGURATION
High light Low light
E4Water Final Conference, Brussels, April 19-20, 2016 19
Flat-panel photobioreactors (0.4 L) 200 and 2100 µmol m-2s-1 PAR Optimal temp and pH maintained by build in feedback systems C. sorokiniana 100 % waste-water Optical density, dry weight and nutrients
REACTOR CONFIGURATION
E4Water Final Conference, Brussels, April 19-20, 2016 20
Photosynthetic yield (n, g mol-1 ), and percentage of light (PAR) absorbed (p, % ).
REACTOR CONFIGURATION
High light Low light
E4Water Final Conference, Brussels, April 19-20, 2016 21
NUTRIENT REMOVAL
E4Water Final Conference, Brussels, April 19-20, 2016 22
Legend. Pigment content in Chlorella sorokiniana biomass. Dilution rate in decreasing order.
BIOREFINERY
E4Water Final Conference, Brussels, April 19-20, 2016 23
Dilution rate is in decreasing order Linoleic acid (18:2) is an omega-6 fatty acid α-linolenic acid (18:3) (ALA) is an omega 3 fatty acid
BIOREFINERY
Fatty acids
E4Water Final Conference, Brussels, April 19-20, 2016 24
UTILIZATION OF ORGANIC CARBON
waste streams offer a low cost, sustainable source of N, P and other minerals
they may also contain organic carbon, which can me metabolized by microalgae
acetate is characteristic of AD effluents
Aim: develop a strategy for adding AD effluent to bioreactor in order to maximize productivity
E4Water Final Conference, Brussels, April 19-20, 2016 25
UTILIZATION OF ORGANIC CARBON
• Strategy 1. Autotrophic: M8a Dilution during light cycle 2. Mixotrophic: + 1 g/L Acetate during light cycle 3. Cyclic Autotrophic/heterotrophic Constant rate: +2 g/L during dark cycle, + acetate free medium during the light. 4. Cyclic Autotrophic/heterotrophic only diluting at night: +2 g/L during dark cycle, no dilution during the day. “enhanced heterotrophy”
E4Water Final Conference, Brussels, April 19-20, 2016 30
BATCH ASSAY
0
175
350
525
700
0 5 11 16 21
Met
hane
yie
ld
mLC
H4/
gVS
Days
Experimental BMP of swine manureExperimental BMP of microalgae
Theoretical BMP of swine manureTheoretical BMP of microalgae
0
175
350
525
700
0 5 11 16 21
Met
hane
yie
ld
mLC
H4/
gVS
Days
80/20 60/40
40/60
Manure /algae ratio
E4Water Final Conference, Brussels, April 19-20, 2016 31
Batch experiments
• The addition of 40% wastewater microalgae in swine manure exhibited the highest methane yield (38% higher compared to swine manure). Further addition affected negatively the biomethanation process.
• Possible synergistic effect (further investigation needed)
Continuous experiments
• Co-digestion of SM/M at a ratio of 60/40 presented 21.6% higher methane yield compared with the corresponding one from the degradation of SM as mono-substrate.
E4Water Final Conference, Brussels, April 19-20, 2016 32
Thank you for your attention
This project has received funding from the European Union’s Seventh Programme for research, technological development and demonstration under grant agreement No 280756.