Delta Regional Monitoring Program - Delta …deltacouncil.ca.gov/sites/default/files/2016/08/Delta RMP...2016/08/01 · Delta Regional Monitoring Program Quality Assurance Program

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DeltaRegionalMonitoringProgram

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QualityAssuranceProgramPlan

Preparedby

ThomasJabusch,DonYee,andAmyFranz

SanFranciscoEstuaryInstitute-AquaticScienceCenter

Version2.2

July29,2016

SanFranciscoEstuaryInstitute-AquaticScienceCenter

4911CentralAvenue

Richmond,CA94804

DeltaRMPQAPPVersion2.2Page2of120

TitleandApproval

For

PROJECTNAME: DeltaRegionalMonitoringProgram

Date: August1,2016

NAMEOFRESPONSIBLEORGANIZATION: SanFranciscoEstuaryInstitute–AquaticScienceCenter(SFEI-ASC)


TableofContents

TitleandApproval...........................................................................................................................2TableofContents.............................................................................................................................4

ListofTables.......................................................................................................................................6ListofFigures......................................................................................................................................6

0. DistributionList........................................................................................................................71. ProjectTask/Organization.......................................................................................................10

1.1. Roles.......................................................................................................................................101.2. PersonsResponsibleforQAPPUpdateandMaintenance......................................................12

2. ProblemDefinition/Background.............................................................................................123. ProgramTasksDescription......................................................................................................13

3.1. WorkStatementandProducts...............................................................................................133.2. EvaluationofMonitoringData...............................................................................................143.3. BeneficalUsesandWaterQualityGoals................................................................................153.4. ConstituentstobeMonitoredandReported.........................................................................243.5. GeographicalandTemporalSetting.......................................................................................303.6. Constraints..............................................................................................................................32

4. DataQualityObjectivesandIndicators,Criteria,andControlProceduresforMeasurementData 36

4.1. FieldQCProcedures...............................................................................................................384.2. LaboratoryPerformanceMeasurementsforChemicalAnalyses...........................................404.3. LaboratoryQualityControlProceduresforChemicalAnalyses..............................................454.4. DataQualityIndicatorsandTestAcceptabilityCriteriaforToxicityTestingandAssociatedWaterQualityMeasurements..........................................................................................................564.5. Performance-basedmethodconceptforthedeterminationofLT2pathogens(CryptosporidiumandGiardia)........................................................................................................66

5. SpecialTrainingNeedsandCertification.................................................................................695.1. SpecializedTrainingorCertifications......................................................................................695.2. TrainingCertificationandDocumentation.............................................................................705.3. TrainingPersonnel..................................................................................................................70

6. DocumentsandRecords.........................................................................................................706.1. ReportPackageInformation...................................................................................................716.2. DataReportingRequirements................................................................................................776.3. DataStorage/Database...........................................................................................................78

7. SamplingProcessDesign.........................................................................................................787.1. StudyAreaandPeriod............................................................................................................787.2. SamplingSites.........................................................................................................................79

8. SamplingMethods..................................................................................................................808.1. FieldEquipmentandSupplies................................................................................................808.2. FieldSampleCollectionandQualityAssuranceProcedures...................................................818.3. CorrectiveAction....................................................................................................................86

9. SampleHandlingandCustody.................................................................................................899.1. FieldSampleHandlingandShippingProcedures...................................................................89

10. AnalyticalMethods...............................................................................................................9210.1. FieldAnalytical/MeasurementMethods..............................................................................92

DeltaRMPQAPPVersion2.2

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10.2. LaboratoryMethods.............................................................................................................9310.3. SampleArchiveandDisposal................................................................................................95

11. Instrument/Equipment/Supplies..........................................................................................9512. Non-directMeasurements(ExistingData).............................................................................9713. DataManagement................................................................................................................9714. AssessmentandResponseActions........................................................................................9915. ReportstoManagement.......................................................................................................9916. DataReview,Verification,andValidation...........................................................................10017. VerificationandValidationMethods...................................................................................10018. ReconciliationwithUserRequirements...............................................................................10419. References..........................................................................................................................10420. Appendices.........................................................................................................................107

20.1. AppendixA.ManagementQuestions.................................................................................10720.2. AppendixB.AssessmentQuestions....................................................................................10820.3. AppendixC.DeltaRMPMonitoringElements....................................................................11220.4. AppendixD.ListofSOPs.....................................................................................................11320.5. AppendixE.ExampleFieldSheets......................................................................................11620.1. AppendixF.ExampleforChainofCustodyForm...............................................................120


ListofTables

Table0.1.DistributionList............................................................................................................................................7Table1.1.Analyticallaboratories...............................................................................................................................11Table3.1.DeltaRMPreportingcycle.........................................................................................................................14Table3.2.BeneficialUsesassociatedwithDeltaRMPmonitoringelements.............................................................16Table3.3.EPAOfficeofWater(OW)AmbientWaterQualityCriteria,EPAOfficeofPesticidePrograms(OPP)

AquaticLifeCriteria,andWaterQualityObjectivesfortargetanalytesofCUPmonitoring............................17Table3.4.Waterqualitybenchmarksformercury.....................................................................................................24Table3.5.BinclassificationforPublicWaterSystems................................................................................................24Table3.6.DeltaRMPtargetparametersandreportingunits....................................................................................25Table3.7.ListofsitetypesamplingfrequenciesandassociatedparametergroupsforCUPmonitoring.................31Table4.1.PurposesoffieldandlaboratoryQCsampletypesanddataqualityindicatorsapplicabletotheDelta

RMP...................................................................................................................................................................36Table4.2.Acceptancecriteriaforfieldmeasurements..............................................................................................39Table4.3.Chemical-analyticalQC..............................................................................................................................42Table4.4.SummaryofReportingLimits(RL)andMethodDetectionLimits(MDL)ofDeltaRMPconstituents........48Table4.5.Summaryofinstrumentrangesandresolutionforlaboratorymeters.....................................................54Table4.6.Recoverysurrogatestandardsusedforpesticideanalysesandassociatedmeasurementquality

objectives..........................................................................................................................................................56Table4.7.Measurementqualityobjectivesfortoxicitytestingandassociatedwaterqualitymeasurements.........57Table4.8.Summaryoftestacceptabilitycriteria.......................................................................................................57Table4.9.QualityControlMeasuresfortoxicitytesting............................................................................................61Table4.10.MeasurementQualityObjectivesfortoxicitytesting..............................................................................63Table4.11.QCrequirementsandacceptancecriteriafordeterminationofCryptosporidiumandGiardiainaqueous

matrices(EPAMethod1623)............................................................................................................................67Table4.12.QCrequirementsandacceptancecriteriafordeterminationofCryptosporidiumandGiardiainaqueous

matrices(EPAMethod1623.1).........................................................................................................................68Table6.1.CEDENcontrolledvocabularyforresultqualifiers.....................................................................................74Table6.2.CommonCEDENQAcodes........................................................................................................................75Table7.1.Samplingsitesandschedule......................................................................................................................79Table8.1.Samplingeventtriggersforpesticideeventssampling.............................................................................82Table8.2.Samplecontainertypeandvolumeusedforeachparametergroupforcollectionofwatersamples.....85Table8.3.Targetspecies,numberofindividuals,andsizerangesforcollectionoffishtissuesamples....................86Table9.1.Storageandholdtimerequirementsforeachparametergroup..............................................................90Table10.1.Summaryofanalyticalmethodsandinstruments...................................................................................93Table10.2.Correctiveactionsproceduresforanalyticallaboratories.......................................................................94Table17.1.ComplianceCodes..................................................................................................................................103Table17.2.BatchVerificationCodes........................................................................................................................103

ListofFigures

Figure1.1.ProjectOrganizationChart.......................................................................................................................10Figure3.1.FY2014-17PesticideWaterSamplingSites..............................................................................................33Figure3.2.FY2016-17MercuryMonitoringSites......................................................................................................34Figure3.3.FY2014-17AmbientPathogenMonitoringSites......................................................................................35


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0. Distribution List

Table0.1.DistributionList.

Name Affiliation Title Phone EmailAddress No.ofCopies

RichardDanielson

BioVirLaboratoryDirector

(800)442-7342 [email protected] 1

JamesTruscott

BioVir QAOfficer (800)442-7342 [email protected] 1

ElbaMoran BioVir ClientRep (800)442-7342 [email protected] 1

SelinaCole CVRWQCBDeltaRMPStaff


PatrickMorris

CVRWQCB ASCContractManager


AlishaWenzel

CVRWQCB

SWAMPRegion5ContractManager


MagnoliaBusse

EurofinsAnalyticalServicesManager


NildaCox Eurofins QAOfficer (626)3861170 [email protected] 1

BrianLaurenson

LWA

DeltaRMPPathogenStudyLiaison

(530)753-6400ext.230

[email protected] 1

WesHeim

MPSL

PI/ProjectManager


AutumnBonnema

MPSL

ProjectCoordinator/QAOfficer

831-771-4175 [email protected] 1

TravisBrown

MWQISampleCollectionTeamLead


ArinConner



JeremyDelCid



StevenSanJulian

MWQIMWQIFieldSectionSupervisor




AdamLaputz

SCRepresentative–Regulatory(State)


GregGearheart

SCRepresentative–Regulatory(State)


TerryFleming

SCRepresentative–Regulatory(Federal)


GreggErickson

SCRepresentative–CoordinatedMonitoring


DaveTamayo

SCRepresentative–Stormwater,PhaseI


BrendanFerry

SCRepresentative–Stormwater,PhaseII


StephanieReyna-Hiestand

SCRepresentative–Stormwater,PhaseII


LindaDorn SC Representative–POTWs


DeedeeAntypas

SCRepresentative–POTWs


JosieTellers

SCRepresentative–POTWs


DavidCory SC Representative–Agriculture


MikeWackman

SCRepresentative–Agriculture

(209)472-7127ext.125

[email protected] 1

ValConnor SCRepresentative–WaterSupply


MelanieOkoro

SCRepresentative–ResourceAgencies


ReneeSpears

StateWaterBoard

QAofficer (916)341-5583 [email protected] 1

MelissaMorris

SWAMP QAofficer (916)-341-5868 [email protected] 1

MarieStillway

UCD-AHPLLaboratoryManager/QAOfficer


SweeTeh UCD-AHPL PI (530)754-8183 [email protected] 1


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GregBrewster

USGSSamplingCoordinator


JosephDomagalski

USGSNAWQALeadScientist


MichelleHladik

USGS Co-PI (916)278-3183 [email protected] 1

MeganMcWayne

USGSLaboratoryManager

(916)278-3127Lab:(916)278-3208

[email protected] 1

JimOrlando

USGS Co-PI(916)278-3271Cell(530)218-7198

[email protected] 1

AmandaEgler

USGS QA/QCOfficer 916-278-3210 [email protected] 1

CoreySanders

USGS DataManager (916)278-3289 [email protected] 1

PhilTrowbridge

SFEI-ASCProgramManager


ThomasJabusch

SFEI-ASCProjectManager


AmyFranz SFEI-ASC DataManager (510)746-7394 [email protected] 1

DonYee SFEI-ASC QAOfficer (510)746-7369 [email protected] 1


1. Project Task/Organization

1.1. RolesAnorganizationalchart,withmonitoringresponsibilitiesnoted,isprovidedinFigure1-1.

Figure1.1.ProjectOrganizationChart.

UnderthedirectionoftheDeltaRegionalMonitoringProgram(DeltaRMP)SteeringCommittee,theTechnicalAdvisoryCommittee(TAC)providestechnicaloversightoftheDeltaRMPandSanFranciscoEstuaryInstitute–AquaticScienceCenter(SFEI-ASC)managesandoperatestheprogramastheimplementingentity.

TheSFEI-ASCProjectManagerisresponsibleforcoordinatingallaspectsofmonitoringcomponentsofthisprojectincludingtheorganizationoffieldsampling,schedulingofsamplingdays,andinteractionswiththecontractlaboratories.TheSFEI-ASCProjectManagerworksincloseconsultationwiththeSFEI-ASCProgramManager.SFEI-ASCProgramManagerandSFEI-


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ASCProjectManagerreportdirectlytotheDeltaRMPSteeringCommittee.Projectplansaretobereviewedannually.

TheSFEI-ASCRegionalDataCenterManagerwillensurethatdatasubmittedbysubcontractorlabsaretimely,complete,andproperlyincorporatedintotheRegionalDataCenterdatabase,forusebystatewidecompilationsofdata,suchasCEDENorMyWaterQualityEstuaryPortal.

SFEI-ASC’sQualityAssuranceOfficer(QAO)roleistoestablishandoverseethequalityassuranceandqualitycontrol(QA/QC)proceduresfoundinthisQAPP,whichincludefieldandlaboratoryactivities.TheSFEIQAOwillworkwiththeQualityAssuranceOfficersforcontractedanalyticallaboratories,reviewingandcommunicatingallQA/QCissuescontainedinthisQAPPtothelaboratories.ContactinformationforkeystaffislistedinTable0.1.

LaboratoriescontractedbySFEI-ASCprovidehighqualityanalyticalservices.TheanalyticallaboratorieswillactasatechnicalresourcetoSFEI-ASCstaffandmanagement.TheresponsiblepersonnelandcontactinformationarelistedaboveinTable0.1.

Table1.1.Analyticallaboratories.

Analyticallaboratory Lababbrev. Matrix Analytical

Services

ELAP/NELAPAccreditation

NumberLabQAManualLink

BioVirLaboratories BioVir WaterCryptosporidium/

Giardia

ELAP

CertificateNo.1795

QualitySystemPlanforEnvironmentalHealth,

Inc.,D/B/ABiovirLaboratories

Eurofins Eurofins WaterCryptosporidium/

Giardia

ELAPCertificateNo.2944/NELAPID:4034

LT2-Giardia/CryptoQAPP

MarinePollutionStudiesLab,MossLandingMarineLabs

MPSLTissue,Water

Fishattributes,mercury,

suspendedsolids

N/A

MPSLLaboratoryQAP,Revision5.February,

20061CADepartmentofFishandWildlife-WaterPollutionControlLab2

WPCL Water

Chlorophylla,dissolvedorganic

carbon

ELAPCertificateNo.1795

N/A3

U.S.GeologicalSurveyOrganicChemistryResearchLaboratory

USGS-OCRL

WaterField

Measurements,Pesticides

N/A N/A4

U.S.GeologicalSurveyNationalWaterQuality

USGS-NWQL

WaterCopper

(dissolved),

QualityAssuranceandQualityControl

1 ContactMPSLLaboratoryQAO(Table0.1)toobtainacopy. 2 PerformsspecificanalyticalservicesonbehalfofMPSL. 3TheWPCLlabQAmanualiscurrentlybeingupdated.Itwillbemadeavailablewhentheupdatesarecomplete.4USGS-OCRLcurrentlyhasnostandalonedocumentdescribinggeneralQAprocedures.TheexistingQAprocedureshavebeenincorporatedintotheDeltaRMPQAPP,asappropriate,andarealsodocumentedinSOPs.


Analyticallaboratory Lababbrev. Matrix Analytical

Services

ELAP/NELAPAccreditation

NumberLabQAManualLink

Laboratory dissolvedandparticulate

organiccarbon

N/A

UniversityofCaliforniaDavis-AquaticHealthProgramLaboratory

UCD-AHPL

Water

Toxicity,TIEs,alkalinity,ammonia,hardness

ELAPCertificateNo.2243

UCDAHPLQAM

1.2. PersonsResponsibleforQAPPUpdateandMaintenance

ChangesandupdatestothisQAPPmaybemadeafterareviewoftheevidenceforchangebySFEI-ASC’sProjectManagerandQAO,andwiththeconcurrenceoftheDeltaRMPTechnicalAdvisoryCommittee.SFEI-ASC’sQAOwillberesponsibleformakingthechanges,submittingdraftsforreview,preparingafinalcopy,andsubmittingthefinalforsignatures.ChangesareexpectedyeartoyearintheearlyyearsofDeltaRMPimplementation.

2. Problem Definition/Background

TheDeltaRMPwasinitiatedin2008bytheCentralValleyRegionalWaterQualityControlBoard(RegionalWaterBoard)withtheprimarygoaloftrackinganddocumentingtheeffectivenessofbeneficialuseprotectionandrestorationeffortsthroughcomprehensivemonitoringofwaterqualityconstituentsandtheireffectsintheDelta.ThedevelopmentoftheDeltaRMPwasinitiallypromptedbythecollapseofthepopulationsofseveralspeciesoffishintheearly2000s,aneventthattriggerednewinquiriesintothepotentialroleofcontaminantsinwhatisnowtermedthePelagicOrganismDecline(POD).However,theseinquirieshighlightedshortcomingsofexistingmonitoringeffortstoaddressquestionsatthescaleoftheDelta.Therecognitionthatdatafromcurrentmonitoringprogramswereinadequateincoverage,couldnoteasilybecombined,andwerenotadequatetosupportarigorousanalysisoftheroleofcontaminantsinthePODpersuadedregulatoryagenciestoimprovecoordinationacrossmultiplemonitoringprograms.

Inaddition,theDeltaRMPreflectsanincreasingdesireamongwaterqualityandresourcemanagersthroughoutthestateformoreintegratedinformationaboutpatternsandtrendsinambientconditionsacrosswatershedsandregions.Manystressorsonbeneficialusesareinterrelatedandmustbeaddressedmoreholistically.TheDeltaRMPcomplementsexistinglarger-scalecollaborativemonitoringeffortsthroughoutthestatethatattempttoaddressquestionsandconcernsaboutregionalconditionsandtrends(e.g.,SanFranciscoBayRMP,SouthernCaliforniaBightMonitoringProgram,SurfaceWaterAmbientMonitoringProgram).

TheDeltaRMPSteeringCommitteeisthedecision-makingbodyoftheDeltaRMP.TheSteeringCommitteeisresponsibleforestablishingtheDeltaRMP’sstrategicdirectionandthepolicies


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andproceduresthatgovernitsoperation.TheSteeringCommitteemaydirectDeltaRMPstaffandadvisorycommitteestoassistinmeetingtheobjectivesandmaydelegateday-to-dayfunctionsoftheDeltaRMPtotheDeltaRMP’simplementingentity.

TheSteeringCommitteeauthorizestheimplementationofagreementsamongtheparticipatingmembersand,specifically:

1. Directsthefiscal/operatingagenttorequestandreceivefederal,state,local,andprivatefundsfromanysourceandtoexpendthosemoneystoaccomplishtheDeltaRMP’sgoals

2. Approvesbudgetsandexpenditures

3. Directsthefiscal/operatingagenttoenterintopartnerships,contracts,andotherlegalagreementsonbehalfoftheDeltaRMP,asnecessarytofulfilltheDeltaRMP’smission

4. ApprovesDeltaRMPworkproductsandanyotherplans,products,orresolutionsoftheDeltaRMP

5. SetsprioritiesandoverseetheactivitiesoftheTechnicalAdvisoryCommittees

6. Establishesandoverseestheimplementationofpoliciesandproceduresnecessarytotheday-to-dayfunctioningoftheDeltaRMP

TheDeltaRMPSteeringCommitteedecidedthattheinitialDeltaRMPwouldfocusonmercury,nutrients,pathogens,andpesticides.ManagementquestionstobeansweredbythemonitoringweredevelopedandprovidedtotheTACtodesignamonitoringprogramthatwouldanswerthemanagementquestionsposedbytheSteeringCommittee.ThisQAPPisaddressingmercury,pathogens,andpesticides.TheDeltaRMPnutrientmonitoringisstillbeingdeveloped.Whenthenutrientmonitoringhasbeendeveloped,thisQAPPwillbeupdatedtoincludenutrientandnutrient-associatedconstituents.

3. Program Tasks Description

3.1. WorkStatementandProducts

Toaddressthemanagementquestionsposed(AppendixA),theDeltaRMPwillconductsamplingforpesticides(monthly),mercury(quarterlyforunfilteredmethylmercuryandassociatedconstituentsinwaterandannuallyforfish),andpathogens(monthly).ThisworkisplannedandperformedundertheguidanceoftheDeltaRMPSteeringCommitteewithtechnicaladviceonmonitoringdesignfromtheTechnicalAdvisoryCommittees,whicharecomposedofstateandfederalregulators,permittees,watersupply,andcoordinatedmonitoringprogramrepresentatives.

DatafromStatusandTrendsmonitoringeffortswillbemadeavailableannuallyfordownloadviatheSFEI-ASCContaminantData,DisplayandDownloadtool(CD3)(http://cd3.sfei.org)andsubsequentlyincorporatedintotheCaliforniaEnvironmentalDataExchangeNetwork(CEDEN)andtheCaliforniaEstuarieswebportal.DatawillbereportedinanAnnualMonitoringReport,


andaninterpretivemainreport(ThePulseofTheDelta)thatwillbepublishedinintervalsdecidedbytheSteeringCommitteetosummarizemonitoringresultsandsynthesizetheinformationtheyprovideinthecontextoftheassessmentandmanagementquestionsthatprovidetheframeworkforthemonitoringprogram.

Table3.1providesasummaryofkeyproductsoftheDeltaRMP.ThePulseoftheDeltawillbethemaininterpretivereportingvehicleforDeltaRMPresults.Theaudienceofthisreportwillbelocal,state,andfederaldecision-makersandtheinterestedpublic.ThedatawillbeinterpretedtoanswerDeltaRMPmanagementandassessmentquestions,basedonthemostappropriatestatisticalanalysestobeusedforevaluatingthedatainrelationtoaquestion,asguidedbytheTAC.BoththeTACandtheSCwillprovidereviewofthePulseoftheDelta.PriortoreleaseofthePulseoftheDelta,SFEI-ASCwillprovidebasicannualdatareports(AnnualMonitoringResultsReport)forreviewbytheTACandSC.Monitoringresultswillbeoneofthemaindecisionfactorsforadaptivechangestothemonitoringprogram.AnannualSCplanningmeeting/workshopwillidentifyadaptationsneededtothemonitoringprogramandwillbeinformedbymonitoringresults.Inaddition,theTACwillhaveaccesstopreliminarydatathroughtheTACwebsiteandthepassword-protecteddata-sharingworkspaceoftheCaliforniaEstuarieswebportal.

Table3.1.DeltaRMPreportingcycle.

Deliverable Frequency Releasedate

Datauploads

Provisionaldata(availabletoTACmembers)

Variable Variable

CD3 Annually1 March1

CEDEN Annually March1

CaliforniaEstuarieswebportal Annually March1

Reports

AnnualMonitoringReports(includingQAreport) Annually March1

TechnicalReports Variable Variable

PulseoftheDelta Variable Fall1Timeperiodofdataforannualreporting:pesticides(15months:July1throughSeptember30ofthefollowingyear),mercury(July1–June30),pathogens(April1–March31).

3.2. EvaluationofMonitoringData

Theprogram’smissionistoinformdecisionsonhowtoprotectandrestorebeneficialusesofwaterintheDelta,byproducingobjectiveandcost-effectivescientificinformationcriticaltounderstandingregionalwaterqualityconditionsandtrends.Dataanalysesandinterpretationin


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theDeltaRMPprovideanswerstotheassessmentquestions,andultimately,themanagementquestions.

Programparticipantsdeveloptheinterpretationcollectivelyinascience-basedandcollaborativeprocess.TheDeltaRMPSteeringCommitteehastheleadroleinmakingstatementsaboutthecoremanagementquestions.WithoversightbytheTAC,programstaffandcontractedindependentscientistsconducttherelevantanalysesbyevaluatingthedataagainstthespecificmonitoringquestionsandanystatedbenchmarksorperformancetargets.Asolidreviewprocessensuresthatinformationgeneratedbytheprogramishighquality,objective,andrelevant.

TheDeltaRMPprovidesdecision-makersandresourcemanagerswithinformationtofocusonwaterqualityproblems,todeterminewhatisandwhatisnotaproblemandfacilitateinformeddecisions.However,decisionsbasedonthedataaboutwhetherthereisimpairmentorwhetherandwhattypesofactionsaretobetakenaremadeoutsideoftheprogram.Regulatorydecisions,suchas303(d)listings,willbemadebytheWaterBoardusingitsownprocess.Therefore,theDeltaRMPdoesnothaveadetailedassessmentframeworkfordatainterpretationandfollow-up.

3.3. BeneficalUsesandWaterQualityGoals

ThecoremanagementquestionsandassessmentquestionscurrentlyencompassthefollowingbeneficialusesintheCentralValleyRegionBasinPlan(BasinPlan,CentralValleyRegionalWaterBoard2011)andtheBay-DeltaWaterQualityControlPlan(Bay-DeltaPlan,StateWaterBoard2006):

• ColdFreshwaterHabitat(COLD)

• CommercialandSportFishing(COMM)

• EstuarineHabitat(EST)

• FishMigration(MIGR)

• MunicipalandDomesticWaterSupply(MUN)

• PreservationofRare,Threatened,orEndangeredSpecies(RARE)

• WaterContactRecreation(REC1)

• NoncontactWaterRecreation(REC2)

• ShellfishHarvesting(SHELL)

• FishSpawning(SPWN)

• WarmFreshwaterHabitat(WARM)

• WildlifeHabitat(WILD)


Table3.2providesanoverviewofbeneficialusesthatarerelevanttotheprioritizedassessmentquestions(AppendixB)ofeachoftheindividualmonitoringelements.Table3.3summarizesexistingnumericwaterqualitycriteriaandaquaticlifebenchmarksfortargetanalytesofpesticidemonitoring.Chemicalspecificnumericcriteriadonotexistforalltargetanalytes.Table3.4liststheregulatorytargetsformethylmercurythatwillbeusedinevaluationsofDeltaRMPdata.Table3.5providesinformationonLT2Rulebinlevelclassificationofsourcewater,basedonCryptosporidiumconcentrations.Binlevelsareusedintriggerexceedanceassessmentsofpathogenmonitoringdata.

Table3.2.BeneficialUsesassociatedwithDeltaRMPmonitoringelements.

BeneficialUse Pesticides Mercury Nutrients1 Pathogens

COLD X X X

COMM X X

EST X X X

MIGR X X

MUN X X

RARE X X X

REC1 X

REC2 X

SHELL X X

SPWN X X

WARM X X X

WILD X X X 1Plannedforfutureimplementation.


Table3.3.EPAOfficeofWater(OW)AquaticLifeAmbientWaterQualityCriteria,EPAOfficeofPesticidePrograms(OPP)AquaticLifeBenchmarks5,andWaterQualityObjectivesfortargetanalytesofpesticidemonitoring(CentralValleyWaterBoard1998,2007;EPA2000,2015a,2015b)(concentrationsinμg/L).

Pesticide

WaterQualityObjectives


OWAquaticLifeCriteria

OPPAquaticLifeBenchmarks(italicized:OPPbenchmarkequivalents,Luoetal.2013)

OPPBenchmarkEquivalents

R5-Delta CAToxicsRule Fish Invertebrates Nonvascularplants

Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteTraceElements

Copper(dissolved) 10 — 13 9 CalculatedusingtheBioticLigandModel 15.7 9.01 2.05 1.11 3.1 2300 —

DegradatesChlorpyrifosOA — — — — — — — — — — — — —Dichlorophenyl-3-methylUrea,3,4- — — — — — — — — — — — — —

DDD(p,p’) — — — — — — — — — — — — —DDE(p,p’) — — — — — — — — — — — — —Dichloroaniline,3,4- — — — — — — — — — — — — —Dichloroaniline,3,5- — — — — — — — — — — — — —DichlorophenylUrea,3,4- — — — — — — — — — — — — —Diazoxon — — — — — — — — — — — — —FipronilDesulfinyl — — — — — — 10 0.59 100 10.3 140 >100 —FipronilDesulfinylAmide — — — — — — — — — — — — —FipronilSulfide — — — — — — 41.5 6.6 1.065 0.11 140 >100 —FipronilSulfone — — — — — — 12.5 0.67 0.36 0.037 140 >100 —Malaoxon — — — — 0.065 0.013 — — — — — — —

5EPA.2015a.AquaticLifeBenchmarksforPesticideRegistration.URL:http://www2.epa.gov/pesticide-science-and-assessing-pesticide-risks/aquatic-life-benchmarks-pesticide-registration#benchmarks.AccessedonJuly8,2016.


Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteTebupirimfosoxon — — — — — — — — — — — — —FungicidesAcibenzolar-S-methyl — — — — — — — — — — — — —Azoxystrobin — — — — — — 235 147 130 44 49 3400 —Boscalid — — — — — — 1350 116 >2665 790 1340 >3900 —Captan — — — — — — 13.1 16.5 4200 560 320 >12700 —Carbendazim — — — — — — 190 — 150 — 7700 — 75Chlorothalonil — — — — — — 5.25 3 1.8 0.6 6.8 630 —Cyazofamid — — — — — — >53.5 90.1 >650 <87 — >1220 —Cymoxanil — — — — — — 29000 — 27000 — 254 — 254Cyproconazole — — — — — — — — — — — — —Cyprodinil — — — — — — 1205 230 16 8 2250 — —Desthio-Prothioconazole — — — — — — — — — — — — —Difenoconazole — — — — — — 405 8.7 385 5.6 98 1900 —Dimethomorph — — — — — — 3100 <341 >5300 110 — — —Ethaboxam — — — — — — — — — — — — —Famoxadone — — — — — — 11 — 12 — 22 — 5.5Fenamidone — — — — — — 370 4.7 24.5 12.5 70 >880 —Fenarimol — — — — — — 450 180 3400 113 100 — —Fenbuconazole — — — — — — 1500 — 2300 — 330 — 330Fenhexamide — — — — — — 670 101 >9400 1000 4820 >2300 —Fluazinam — — — — — — 18 0.69 90 68 1.1 — —Fludioxonil — — — — — — 235 19 450 <19 70 >1000 —Fluopicolide — — — — — — 174.5 151 >850 190 <1.4 >3200 —Fluoxastrobin — — — — — — 435 — 480 — 350 — 217.5


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Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteFlusilazole — — — — — — — — — — — — —Flutolanil — — — — — — 1250 233 >3400 530 8010 8010 —Flutriafol — — — — — — 16500 4800 33550 310 460 780 —Fluxapyroxad — — — — — — — — — — — — —Imazalil — — — — — — 1480 — 3500 — 870 — 740Ipconazole — — — — — — 765 0.18 850 — — — —Iprodione — — — — — — — 260 120 — >130 >12640 —Kresoxim-methyl — — — — — — 95 87 166 55 29.2 >301 —Mandipropamid — — — — — — — 220 3550 — >2500 >7400 —Metalaxyl — — — — — — 65000 9100 14000 100 140000 92000 —Metconazole — — — — — — 2100 — 4200 — 1700 — 1050Myclobutanil — — — — — — 1200 980 5500 — 830 — —Paclobutrazol — — — — — — 7950 49 120 9 40800 8 —PCNB — — — — — — 50 13 385 18 — — —Picoxystrobin — — — — — — 32.5 36 12 1 4 210 —Propiconazole — — — — — — 425 95 650 260 21 4828 —Pyraclostrobin — — — — — — 3.1 2.35 7.85 4 1.5 1720 —Pyrimethanil — — — — — — 5050 20 1500 1000 1800 7800 —Quinoxyfen — — — — — — — — — — — — —Sedaxane — — — — — — — — — — — — —Tebuconazole — — — — — — 1135 12 1440 120 1450 151.5 —Tetraconazole — — — — — — 1925 300 1315 190 — 310 —Thiabendazole — — — — — — 280 110 155 42 3060 2320 —Triadimefon — — — — — — 2050 41 800 52 17000 — —Triadimenol — — — — — — — — — — — — —


Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteTrifloxystrobin — — — — — — 7.15 4.3 12.65 2.76 37.1 >1930 —Triflumizole — — — — — — 290 33 700 67 140 720 —Triticonazole — — — — — — — — — — — — —Zoxamide — — — — — — 78 3.48 >390 39 10 19 —HerbicidesAlachlor — — — — — — 900 187 1250 110 1.64 2.3 —Atrazine — — — — — — 2650 — 360 60 <1 0.001 —Benfluralin — — — — — — — — — — — — —Butralin — — — — — — — — — — — — —Butylate — — — — — — 105 — 5950 — — — —Clomazone — — — — — — 1450 350 2700 2200 167 30200 —Cycloate — — — — — — 2250 — 1300 — — — —Cyhalofop-butyl — — — — — — 790 — 2700 — 960 — 395Dacthal — — — — — — 15000 — 13500 — >11000 >11000 —Dithiopyr — — — — — — — — — — — — —Diuron — — — — — — 200 26.4 80 200 2.4 15 —EPTC — — — — — — 7000 — 3250 800 1,400 5600 —Ethalfluralin — — — — — — 16 0.4 30 24 25 — —Flufenacet — — — — — — — — — — — — —Fluridone — — — — — — 2800 480 680 — — — —Hexazinone — — — — — — 137000 17000 75800 20000 7 37.4 —Metolachlor — — — — — — 1600 30 550 1 8 21 —Molinate — — — — — — 105 390 170 340 220 3300 —Napropamide — — — — — — 3200 1100 7150 1100 3400 — —Novaluron — — — — — — >490 6.16 0.075 0.03 3549 >75.4 —


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Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteOryzalin — — — — — — 1440 220 750 358 42 >15.4 —Oxadiazon — — — — — — 600 33 1090 33 5.2 41 —Oxyfluorfen — — — — — — 100 1.3 750 13 1.1 0.33 —Pebulate — — — — — — 3150 — 3,315 — 230 1800 —Pendimethalin — — — — — — 69 6.3 140 14.5 5.2 12.5 —Penoxsulam — — — — — — >51000 10200 >49250 2950 92 3 —Prodiamine — — — — — — >6.5 — >6.5 1.5 — — —Prometon — — — — — — 6000 19700 12850 3450 98 — —Prometryn — — — — — — 1455 620 4850 1000 1.04 11.9 —Propanil — — — — — — 1150 9.1 600 86 16 110 —Pronamide — — — — — — 36000 7700 >2800 600 >4000 1180 —Simazine — — — — — — 3200 — 500 — 2.24 140 —Thiazopyr — — — — — — 3400 — 6100 — 40 — 40Thiobencarb — — — — — — 220 21 50.6 1.0 17 770 —Triallate — — — — — — 600 38 45.5 14 21 2400 —TributhylPhosphorotrithioate,S,S,S-

— — — — — — 122.5 3.5 3.4 1.56 148 1100 —

Trifluralin — — — — — — 20.5 1.14 280 2.4 7.52 43.5 —InsecticidesAcetamiprid — — — — — — >50000 19200 10.5 2.1 >1000 >1000 —Allethrin — — — — — — — — 1.05 — — — —AzinphosMethyl — — — — — — 0.18 0.055 0.08 0.036 — — —Bifenthrin — — — — — — 0.075 0.04 0.8 0.013 — — —Carbaryl — — 2.1 2.1 2.1 2.1 110 6 0.85 0.5 660 1500 —


Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteCarbofuran — — — — — — 44 5.7 1.115 0.75 — — —Chlorantraniliprole — — — — — — >600 110 4.9 4.5 1800 2000 —Chlorpyrifos 0.025 0.015 — — 0.083 0.041 0.9 0.57 0.05 0.04 140 — 0.025Clothianidin — — — — — — >50750 9700 11 11 64000 121000 —Coumaphos — — — — — — 140 11.7 0.037 0.0337 — — —Cyantranilipole — — — — — — >5000 10700 10.2 6.56 >10000 12100 —Cyfluthrin,Total — — — — — — 0.034 0.01 0.0125 0.0074 >181 — —Cyhalothrin,Total — — — — — — — — — — — — —Cypermethrin,Total — — — — — — 0.195 0.14 0.21 0.069 — — —DDT(p,p') — — 1.1 0.001 1.1 0.001 — — — — — — —Deltamethrin — — — — — — 0.29 0.017 0.055 0.0041 — — —Diazinon 0.16 0.1 — — 0.17 0.17 45 <0.55 0.105 0.17 3700 — 0.16Dinotefuran — — — — — — >49550 >6360 >484150 >95300 >97600 >110000 —Esfenvalerate — — — — — — 0.035 0.035 0.025 0.017 — — —Ethofenprox — — — — — — 1.35 23 0.4 0.17 >18.8 >26 —Fenpropathrin — — — — — — 1.1 0.091 0.265 0.064 — — —Fenpyroximate — — — — — — 0.22 0.11 0.8 0.56 1.9 >190 —Fenthion — — — — — — 415 7.5 2.6 0.013 400 >2800 —Fipronil — — — — — — 41.5 6.6 0.11 0.011 140 >100 —Flonicamid — — — — — — 100000 — 100000 — 3300 — 3300Imidacloprid — — — — — — >41500 1200 34.5 1.05 >10000 — —Indoxacarb — — — — — — 145 150 300 75 >110 >84 —Malathion — — — — — 0.1 16.5 8.6 0.295 0.035 2400 >9630 —Methidathion — — — — 0.065 0.013 1.1 6.3 1.5 0.66 — — —Methoprene — — — — — — 380 48 165 51 — — —


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Pesticide







Vascularplants

Lowestreported

Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Chronic Acute Acute AcuteMethoxyfenozide — — — — — — >2100 530 25 6.3 >3400 — —Parathion,Methyl — — — — — — 925 <10 0.485 0.25 15000 18000 —Pentachloroanisole — — — — — — 28 — 150 — — — —Permethrin,Total — — — — — — 0.395 0.0515 0.0106 0.0014 68 — —Phenothrin — — — — — — — — — — — — —Phosmet — — — — — — 35 3.2 1 0.8 — — —Propargite — — — — — — 59 16 37 9 66.2 75000 —Pyridaben — — — — — — — — — — — — —Resmethrin — — — — — — 0.14 0.35 1.55 — — — —Tebupirimfos — — — — — — 44.5 130 0.039 0.011 630 8800 —Tefluthrin — — — — — — 0.03 0.004 0.035 0.008 — — —Tetradifon — — — — — — — — — — — — —Tetramethrin — — — — — — 1.85 — 22.5 — — — —T-Fluvalinate — — — — — — — — — — — — —Thiacloprid — — — — — — 12600 918 18.9 0.97 45000 >95400 —Thiamethoxam — — — — — — >50000 20000 17.5 — >97000 >90000 —Tolfenpyrad — — — — — — — — — — — — —PlantGrowthRegulatorsFlumetralin — — — — — — — — — — — — —SynergistsPiperonylButoxide — — — — — — 950 40 255 30 — — —

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Table3.4.Waterqualitybenchmarksformercury(CentralValleyWaterBoard2011).

Constituent


CentralValleyBasinPlan/

Sacramento-SanJoaquinDeltaandYoloBypasswaterways

Muscletissueoftrophiclevel4fish

(mg/kg,wetweight)

Water(unfiltered)

TMDLimplementationgoal

(ng/L)

Mercury,Methyl 0.246 0.06

Table3.5.BinclassificationforPublicWaterSystems(EPA2013).

Cryptosporidiumbinconcentration

(oocysts/L)

Binclassification

<0.075 Bin1

>0.075,<1 Bin2

>1,<3 Bin3

>3 Bin4

3.4. ConstituentstobeMonitoredandReported

DeltaRMPmonitoringwillincludethecollection,measurement,andreportingofmany

parameters.Thefollowinginformationwillbeincludedwitheachsamplecollection:

• Sitelocation(latitudeandlongitude)(Tables7.1)

• Sitesamplingdateandtime(Tables7.1)

• Matrixsampled(e.g.,water)

• Parametermeasurements(Table3.6)

• Collectionandanalyticalmethods(Table4.4)

• Qualifiersandcomments(appliedbyanalyticallabsorbyDeltaRMPstaffindata

review)(Table6.1)

ThecurrentimplementationoftheDeltaRMPincludesmonitoringforpesticides,mercury,and

pathogens.Thus,theQAPPonlyaddressesthepesticides,mercury,andpathogensmonitoring

6Totalmercuryconcentrationsareusedasasurrogateformercury,methylconcentrationsinfishtissue.

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elements.Thepesticidesmonitoringelementincludeschemicalanalysesandtoxicitytesting.

Thechemicalanalytegroupsforthismonitoringelementincludeseveralpesticidegroups,

dissolvedcopper,andancillaryparameterssuchasdissolved/particulateorganiccarbonand

hardness.Themercurymonitoringelementincludestheanalysisofsamplesfromwaterand

fish.Table3.6providesacompletelistoftargetparametersforthecurrentimplementationof

theDeltaRMP.

Table3.6.DeltaRMPtargetparametersandreportingunits.Allparameterslistedunder

pesticidesamplingwillbeanalyzedforeachpesticidesamplingsiteateachpesticidesampling

event.Mercuryfishtissueparameterswillbeanalyzedannuallyandmercurywatersampling

parameterswillbeanalyzedquarterly.Pathogenmonitoringparameterswillbeanalyzedfor

eachpathogenmonitoringsiteateachmonthlysamplingevent.

PesticideSampling

Constituent ReportingGroup Matrix Unit

Oxygen,Dissolved FieldMeasurements Water mg/L

Oxygen,Dissolved FieldMeasurements Water %saturation

pH FieldMeasurements Water pH

SpecificConductivity FieldMeasurements Water μS/cm

Temperature FieldMeasurements Water °CTurbidity FieldMeasurements Water FNU

PesticideSampling–ToxicityTestingLaboratoryAnalysis


HardnessasCaCO3 Conventional Water mg/L

Ceriodaphniadubia(Reproduction) WaterColumnToxicity Wateryoung/originalorganisms

exposed

Ceriodaphniadubia(Survival) WaterColumnToxicity Water %

Hyalellaazteca(Survival)7 WaterColumnToxicity Water %

Pimephalespromelas(Larvalbiomass)

WaterColumnToxicity Watermg/originalorganisms

exposed

Pimephalespromelas(Larvalsurvival) WaterColumnToxicity Water %

Selenastrumcapricornutum(Growth) WaterColumnToxicity Water cells/mL

PesticideSampling–ChemicalAnalysisLaboratory


DissolvedOrganicCarbon(DOC) Conventional Water mg/L



ParticulateOrganicCarbon(POC) Conventional Water mg/L

TotalSuspendedSolids(TSS) Conventional Water mg/L

Copper(dissolved) TraceMetals Water ug/L

7InclusionofHyalellawatertoxicitytestingispendingafinaldecisionbytheSC.

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ChlorpyrifosOxon Degradates Water ng/L

Dichlorophenyl-3-methylUrea,3,4- Degradates Water ng/L

DDD(p,p') Degradates Water ng/L

DDE(p,p') Degradates Water ng/L

Diazoxon Degradates Water ng/L

Dichlorobenzenamine,3,4- Degradates Water ng/L

Dichloroaniline,3,5- Degradates Water ng/L

DichlorophenylUrea,3,4- Degradates Water ng/L

FipronilDesulfinyl Degradates Water ng/L

FipronilDesulfinylAmide Degradates Water ng/L

FipronilSulfide Degradates Water ng/L

FipronilSulfone Degradates Water ng/L

Malaoxon Degradates Water ng/L

Tebupirimfosoxon Degradates Water ng/L

Acibenzolar-S-methyl Fungicides Water ng/L

Azoxystrobin Fungicides Water ng/L

Boscalid Fungicides Water ng/L

Captan Fungicides Water ng/L

Carbendazim Fungicides Water ng/L

Chlorothalonil Fungicides Water ng/L

Cyazofamid Fungicides Water ng/L

Cymoxanil Fungicides Water ng/L

Cyproconazole Fungicides Water ng/L

Cyprodinil Fungicides Water ng/L

Dimethomorph Fungicides Water ng/L

Desthio-Prothioconazole Fungicides Water ng/L

Difenoconazole Fungicides Water ng/L

Ethaboxam Fungicides Water ng/L

Famoxadone Fungicides Water ng/L

Fenamidone Fungicides Water ng/L

Fenarimol Fungicides Water ng/L

Fenbuconazole Fungicides Water ng/L

Fenhexamid Fungicides Water ng/L

Fluazinam Fungicides Water ng/L

Fludioxonil Fungicides Water ng/L

Fluopicolide Fungicides Water ng/L

Fluoxastrobin Fungicides Water ng/L



Flusilazole Fungicides Water ng/L

Flutolanil Fungicides Water ng/L

Flutriafol Fungicides Water ng/L

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Fluxapyroxad Fungicides Water ng/L

Imazalil Fungicides Water ng/L

Ipconazole Fungicides Water ng/L

Iprodione Fungicides Water ng/L

Kresoxim-methyl Fungicides Water ng/L

Mandipropamid Fungicides Water ng/L

Metalaxyl Fungicides Water ng/L

Metconazole Fungicides Water ng/L

Myclobutanil Fungicides Water ng/L

Paclobutrazol Fungicides Water ng/L

PCNB Fungicides Water ng/L

Picoxystrobin Fungicides Water ng/L

Propiconazole Fungicides Water ng/L

Pyraclostrobin Fungicides Water ng/L

Pyrimethanil Fungicides Water ng/L

Quinoxyfen Fungicides Water ng/L

Sedaxane Fungicides Water ng/L

Tebuconazole Fungicides Water ng/L

Tetraconazole Fungicides Water ng/L

Thiabendazole Fungicides Water ng/L

Triadimefon Fungicides Water ng/L

Triadimenol Fungicides Water ng/L

Trifloxystrobin Fungicides Water ng/L

Triflumizole Fungicides Water ng/L

Triticonazole Fungicides Water ng/L

Zoxamide Fungicides Water ng/L

Alachlor Herbicides Water ng/L

Atrazine Herbicides Water ng/L

Benfluralin Herbicides Water ng/L

Butralin Herbicides Water ng/L

Butylate Herbicides Water ng/L

Clomazone Herbicides Water ng/L

Cycloate Herbicides Water ng/L

Cyhalofop-butyl Herbicides Water ng/L

Dacthal Herbicides Water ng/L

Dithiopyr Herbicides Water ng/L

Diuron Herbicides Water ng/L



EPTC Herbicides Water ng/L

Ethalfluralin Herbicides Water ng/L

Flufenacet Herbicides Water ng/L

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Fluridone Herbicides Water ng/L

Hexazinone Herbicides Water ng/L

Metolachlor Herbicides Water ng/L

Molinate Herbicides Water ng/L

Napropamide Herbicides Water ng/L

Novaluron Herbicides Water ng/L

Oryzalin Herbicides Water ng/L

Oxadiazon Herbicides Water ng/L

Oxyfluorfen Herbicides Water ng/L

Pebulate Herbicides Water ng/L

Pendimethalin Herbicides Water ng/L

Penoxsulam Herbicides Water ng/L

Prodiamine Herbicides Water ng/L

Prometon Herbicides Water ng/L

Prometryn Herbicides Water ng/L

Propanil Herbicides Water ng/L

Pronamide Herbicides Water ng/L

Simazine Herbicides Water ng/L

Thiazopyr Herbicides Water ng/L

Thiobencarb Herbicides Water ng/L

Triallate Herbicides Water ng/L

TributylPhosphorotrithioate,S,S,S- Herbicides Water ng/L

Trifluralin Herbicides Water ng/L

Acetamiprid Insecticides Water ng/L

Allethrin Insecticides Water ng/L

AzinphosMethyl Insecticides Water ng/L

Bifenthrin Insecticides Water ng/L

Carbaryl Insecticides Water ng/L

Carbofuran Insecticides Water ng/L

Chlorantraniliprole Insecticides Water ng/L

Chlorpyrifos Insecticides Water ng/L

Clothianidin Insecticides Water ng/L

Coumaphos Insecticides Water ng/L

Cyantraniliprole Insecticides Water ng/L

Cyfluthrin,Total Insecticides Water ng/L

Cyhalothrin Insecticides Water ng/L

Cypermethrin,Total Insecticides Water ng/L



DDT(p,p') Insecticides Water ng/L

Deltamethrin Insecticides Water ng/L

Diazinon Insecticides Water ng/L

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Dinotefuran Insecticides Water ng/L

Esfenvalerate Insecticides Water ng/L

Ethofenprox Insecticides Water ng/L

Fenpropathrin Insecticides Water ng/L

Fenpyroximate Insecticides Water ng/L

Fenthion Insecticides Water ng/L

Fipronil Insecticides Water ng/L

Flonicamid Insecticides Water ng/L

Imidacloprid Insecticides Water ng/L

Indoxacarb Insecticides Water ng/L

Malathion Insecticides Water ng/L

Methidathion Insecticides Water ng/L

Methoprene Insecticides Water ng/L

Methoxyfenozide Insecticides Water ng/L

Parathion,Methyl Insecticides Water ng/L

Pentachloroanisole Insecticides Water ng/L

Permethrin,Total Insecticides Water ng/L

Phenothrin Insecticides Water ng/L

Phosmet Insecticides Water ng/L

Propargite Insecticides Water ng/L

Pyridaben Insecticides Water ng/L

Resmethrin Insecticides Water ng/L

Tebupirimfos Insecticides Water ng/L

Tefluthrin Insecticides Water ng/L

Tetradifon Insecticides Water ng/L

Tetramethrin Insecticides Water ng/L

T-Fluvalinate Insecticides Water ng/L

Thiacloprid Insecticides Water ng/L

Thiamethoxam Insecticides Water ng/L

Tolfenpyrad Insecticides Water ng/L

Flumetralin PlantGrowthRegulators Water ng/L

PiperonylButoxide Synergists Water ng/L

Mercury–FishSampling

Constituent/Measurement ReportingGroup Matrix Unit

Mercury TraceMetalsTissue

(filletmuscle)μg/gww

TotalLength FishAttributes Tissue mm

Mercury–FishSampling


ForkLength FishAttributes Tissue mm

Weight FishAttributes Tissue g

Sex FishAttributes Tissue male/female

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Moisture FishAttributes Tissue %

Mercury-WaterSampling


Mercury,Methyl,total(unfiltered) TraceMetals Water ng/L

Mercury,Methyl,(filtered) TraceMetals Water ng/L

Mercury(unfiltered) TraceMetals Water ng/L

Mercury(filtered) TraceMetals Water ng/L

Chlorophylla Conventional Water μg/L

DissolvedOrganicCarbon(DOC) Conventional Water mg/L

TotalSuspendedSolids(TSS) Conventional Water mg/L

TSS(volatile) Conventional Water mg/L

Oxygen,Dissolved FieldMeasurements Water mg/L

Oxygen,Dissolved FieldMeasurements Water %saturation

pH FieldMeasurements Water pH

SpecificConductivity FieldMeasurements Water μS/cm

PathogenMonitoring


Cryptosporidium Pathogens Water oocysts/L

Giardia Pathogens Water cysts/L

3.5. GeographicalandTemporalSetting

ThegeographicscopeoftheDeltaRMPencompassesthelegalDelta(asdefinedbysection

12220oftheWaterCode),includingwaterbodiesthatdirectlydrainintotheDelta,Yolo

Bypass,andSuisunBay.Inaddition,thebasemonitoringandspecialstudiesoftheDeltaRMP

mayextendupstreamordownstream,ifrequiredtoaddressspecificmanagementquestions.

Monitoringsitesforpesticides8,mercury,andpathogensaredescribedinthissection.

Additionalinformationfornutrientsmonitoringsiteswillbeaddedlater.

3.5.1. Pesticides

Thesurfacewatersamplesforpesticideanalysesarecollectedfromfixedsitesrepresentingkey

inflowstotheDeltathatarevisitedmonthly.Targetedeventsamplinginanygivenmonthmay

beconductedinlieuofscheduledmonthlysampling.Targetedeventsincludetwowetevents

(1stseasonalflush,2ndsignificantstorminwinter)andthreedryevents(earlyspring,irrigation

seasonsamplinglatespring/earlysummer,irrigationseasonsamplinglatesummer).

Figures3.1showsthecurrentwatersamplingsitesandTable3.7providesanoverviewofthe

samplingscheduleforpesticides.Thepesticidesmonitoringelementincludeschemicalanalyses

8Pesticidemonitoringincludeschemicalpesticideanalysis,toxicitytesting,andtheanalysisofdissolvedcopperandrelevantfieldand

conventionalwaterqualityparametersatallsites.

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andtoxicitytesting.Theparametersanalyzedforthismonitoringelementincludeseveral

pesticidereportinggroups,dissolvedcopper,fieldparameters,and“conventional”parameters

(ancillaryparametersmeasuredinthelaboratory,suchasdissolved/particulateorganiccarbon

andhardness).

Table3.7.Listofsitetypesamplingfrequenciesandassociatedparametergroupsforpesticides

monitoring.

ParameterGroup Baselinesitesamplingfrequency Matrix

Conventionalparameters Monthly Water

Fieldparameters Monthly Water

Metals(dissolvedCopperonly) Monthly Water

Pesticides Monthly Water

Watercolumntoxicity Monthly Water

3.5.2. Mercury

Thesurfacewatersamplesformercuryanalysesarecollectedquarterlyfromfixedsitesthat

alignwithsportfishmonitoringsites.

Thesportfishsamplesformercuryanalysesarecollectedannuallyfromfixedsitesthat

representdifferentsubareasoftheDelta.

Figure3.2showsthemercurysamplingsites.Themercurymonitoringelementincludesfishand

watersampling.Thechemicalanalytegroupsforthismonitoringelementincludemercuryand

methylmercuryandancillaryparameterssuchaschlorophylla,DOC,totalsuspendedsolids,andvolatilesuspendedsolids.

3.5.3. Pathogens

Ambientpathogenmonitoringsitesareco-locatedwithexistingsitesoftheMunicipalWater

QualityInvestigations(MWQI)program(Figure3.3).Someofthesesitesareupstreamofthe

Delta,butcouldinfluencewaterqualityatthedrinkingwaterintakesorarerepresentativeof

largerareaswiththesamelanduses.AdditionalsampleswillbecollectedatvariousDelta

watersupplyintakes(7drinkingwaterintakesiteswithasinglesource,plus2facilitieswith

blendingfrom4drinkingwaterintakes)incoordinationwiththeseambientsites.

TheDeltaRMPPathogenStudyDesignspecifiesmonthlyambientmonitoringsamplecollection

fortwoyearsbeginninginApril2015tomatchtheLongTerm2EnhancedSurfaceWater

TreatmentRule(LT2)-requiredwatersupplyintakesamplecollection.MWQIwillcollectgrab

samplesateachofthelocationsshowninTableA-1duringthefirstweekofeachmonthonthe

site-specificday.

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3.6. Constraints

Theabilitytomeasuresomeofthetargetcompoundsattheultra-tracelevelsfoundinthe

ambientenvironmentmaybeconstrainedbythedetectionlimitsroutinelyachievableby

analyticallaboratories.Targetdetectionlimitsinthisdocumentrepresentthoseachievedby

laboratoriescontractedbytheDeltaRMPorlevelsneededtoobtainquantitative

measurementsofambientconcentrationsinamajorityofsamples.

Anotherconstraintisthatdiscretesamplesrepresentonlyamomentintimeandmaytherefore

notalwaysrepresentconditionsduringothertimeperiods.

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Figure3.1.FY2014-17PesticideWaterSamplingSites9.

9 Pesticidemonitoringincludeschemicalpesticideanalysis,toxicitytesting,andtheanalysisofdissolvedcopperandrelevantfieldand

conventionalwaterqualityparametersatallsites.

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Figure3.2.FY2016-17MercuryMonitoringSites.

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Figure3.3.FY2014-17AmbientPathogenMonitoringSites.

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4. Data Quality Objectives and Indicators, Criteria, and Control Procedures for Measurement Data

DataQualityObjectives(DQOs)aimtosupportdefensibleconclusionsthataddressthe

managementquestionsandassessmentquestionsinAppendicesAandB.Dataquality

indicators(DQIs)forfieldandlaboratorymeasurementsevaluatethefollowing:

• Fieldmeasurements–sensitivity,precision,accuracy,completeness

• Laboratorychemicalanalyses–sensitivity,precision,accuracy,completeness,

contamination

• Toxicitytesting–precision,completeness,representativeness

Thediscussioninthissectionreviewsthemeasurementsandproceduresexpectedto

demonstratethequalityofreporteddata.Table4.1providesanoverviewofqualitycontrol

(QC)sampletypesandtheirpurpose.Thequalityassessmentprocessthatisusedafterthedata

havebeencollectedtoevaluatewhethertheDataQualityObjectives(DQOs)havebeen

satisfiedisdescribedandillustratedinSection17,VerificationandValidationMethods.

Table4.1.PurposesoffieldandlaboratoryQCsampletypesanddataqualityindicators

applicabletotheDeltaRMP

QCSampleType DataQualityIndicator/Purpose

CalibrationAccuracyofmeasurement(fieldparameters,laboratorychemical

analysis).

CalibrationCheck Accuracyofcalibration(fieldparameters,laboratorychemicalanalysis).

LaboratoryBlanks-

MethodBlanks

Contamination/confirmtheabsenceofanalytesintroducedinthelab

(laboratorychemicalanalysis).

LaboratoryBlanks-

InstrumentBlanks

Contamination/Assessthepresenceorabsenceofinstrument

contamination(laboratorychemicalanalysis).

CRM(Reference

Material)

Accuracyofmeasurement(primarily);precision/mostrobustindicatorof

measurementaccuracy;mayalsobeusedtoevaluatereplicateprecision

andrecoverywhereaveragevaluesforfieldsamplesareexpected(based

onhistoricalorliteratureresults)tofallinanon-quantitativerange

(laboratorychemicalanalysis).

LaboratoryDuplicates-

MatrixSpikes

(MS)/MatrixSpike

Duplicates(MSD)

Accuracyandprecision/evaluatetheeffectofthesamplematrixonthe

recoveryofthecompound(s)ofinterestandprovidinganestimateof

analyticalprecisionwhenmeasuredinduplicate(laboratorychemical

analysis).

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QCSampleType DataQualityIndicator/Purpose

LaboratoryDuplicates-

MatrixDuplicates

Precisionofintra-laboratoryanalyticalprocess(laboratorychemical

analysis)

SurrogateSpikesAccuracyofanalyticalmethod/assesstheefficiencyoftheextraction

methodfororganicanalytes(laboratorychemicalanalysis).

InternalStandards

Accuracyofanalyticalmethod/enableoptimalquantitation,particularly

ofcomplexextractssubjecttoretentiontimeshiftsorinstrument

interferencesrelativetotheanalysisofstandards.Internalstandardscan

alsobeusedtodetectandcorrectforproblemsintheinjectionportor

otherpartsoftheinstrument(laboratorychemicalanalysis).

FieldBlanks

Contamination/Tocheckcross-contaminationduringsamplecollection,

fieldsampleprocessing,andshipment.Alsotochecksamplecontainers

(laboratorychemicalanalysis).Fieldcrewswillneedtoincludefiltrationin

processingblanksforapplicablesampletypes.

Field

Duplicate/Replicate

Precision/Checkreproducibilityoffieldprocedures.Toindicatenon-

homogeneity.(FieldDuplicate:n=2;FieldReplicate:n>2).Thissampleis

tobecollectedinthefieldintandemwitharegularenvironmental

sample.Tobepreserved,handledandprocessedasauniquesample.Lab

precisioniscoveredbelow(laboratorychemicalanalysis).

InstrumentReplicates Precisionofinstrument(laboratorychemicalanalysis).

Travel/bottleblanks

Contamination/Toaccountforcontaminantsintroducedduringthe

transportprocessbetweenthelaboratoryandfieldsite,inadditiontoany

contaminationfromthesourcesolutionandcontainer(laboratory

chemicalanalysis).

NegativeControlToevaluatetestperformanceandthehealthandsensitivityofthe

specificbatchoforganisms(laboratorytoxicitytesting).

Referencetoxicant

testing

Sensitivity,precisionandaccuracyoftoxicitytestsperformedinthe

laboratory/Determinethesensitivityofthetestorganismsovertime;

assesscomparabilitywithinandbetweenlaboratorytestresults;identify

potentialsourcesofvariability,suchastestorganismhealth,differences

amongbatchesoforganisms,changesinlaboratorywaterorfoodquality,

andperformancebylaboratoryanalysts(laboratorytoxicitytesting).

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4.1. FieldQCProcedures

4.1.1. FieldPerformanceMeasurements

Sensitivityistheabilityofameasurementtodetectsmallquantitiesofthemeasured

component.Thesensitivityoffieldmeasurementsisgenerallydeterminedbytheoutputofthe

analyticalinstrument.Appropriateinstrumentsand/orinstrumentsettingsshouldbechosen

thatgenerallyallowdifferencesbetweensitesorwithinasiteatdifferenttimesbeingreported.

Resolutionontheorderofapproximately1%ofthemaximumorrangeofmeasurementslikely

tobeencounteredisdesired.

Precisionoffieldmeasurementsisdeterminedbyrepeatedmeasurementofthesame

parameterwithinasinglesample,orsamplestakeninrapidsuccession(onlywhenconditions

arenotdynamicallyvariable).Approximately10%ofmeasurements,aminimumofone

measurementperevent,shouldberepeatedforallmeasuredparameters.Repeated

measurementmayalsobeaccomplishedbycontinuousloggingofinsituprobesormeters.

Accuracyoffieldmeasurementsisestablishedbyperiodicmeasurementofknownstandardsor

byrecalibrationtoknownstandards.Instrumentrecalibrationshouldbeperformedpriorto

eachsamplingdayoreventforuser-calibratedinstruments(e.g.dailyforhandheldfield

meters),oratthemanufacturer-specifiedintervalforinstrumentsrequiringfactoryservicingor

otherwiseincapableoffieldrecalibration.

Completenessoffieldmeasurementisevaluatedasapercentageofusablemeasurementsout

ofthetotalnumberofmeasurementsdesired.Morethan90%offieldmeasurementsshouldbe

usable.Ifalowerpercentageisachievedforanysamplingeventortimeperiod,causesshallbe

investigatedandfixedwherepossible,throughinstrumentmaintenance(e.g.defouling),

recalibration,repair,orreplacement(withthesameordifferentinstrumenttype)asneeded.If

completenesstargetsarenotachieved,instrumentchoice,settings,deploymentmethod,

maintenance,and/orotheractivitiesshallbeadjustedtoimprovemeasurementreliability

beforethenextsamplingeventormeasurementperiod.

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4.1.2. FieldQCMeasurements

Calibrationofanyfieldmeters(pH,conductivity,DO,orothermeasurements)shouldbe

checkedinthefieldatleastoncedailyandrecalibratedusingcertifiedstandardsorprocedures

wherepossible.Instrumentswillberecalibratedwhensignificantdriftoracalibrationerroris

found.

Beyondinitialcalibrationofhandheldfieldinstrumentsandperiodiccalibrationchecksinthe

field,QCmeasurestakenforfieldinstrumentmeasurementsshouldincludereportingof

replicates.FieldmeasurementacceptancecriteriaaresummarizedinTable4.2.

Table4.2.Acceptancecriteriaforfieldmeasurements.

Method Parameters Sampletype Matrix Frequency Acceptablelimits

YSI6920Water

QualityMeter

DO,pH,EC,

temperature,

turbidity

Calibration Water

Within24hrs

beforesampling

aswellasamid-

dayandend-of-

the-daychecks

againstthe

standards

Allowabledrift+

10%forDOand

Specific

Conductivity,+

0.2forpH,+5

turbidityunitsor

+5%ofthe

measuredvalue

(whicheveris

greater)for

turbidity

YSI6920Water

QualityMeterSC

Instrument

BlankWater 1per20sitevisits <MDLforSC

4.1.3. FieldQCSamples

FieldQCsamplesthatarefrequentlycollectedforlaterlabanalysisinsamplingprotocolsare

listedbelow.FieldQCsamplesareanalyzedforalltargetanalytesofasamplingevent.Field

blanksandfieldduplicates/replicateswillbecollectedatafrequencyofoneper20samples(5%

ofsamples)orperbatch/event,whicheverismorefrequent:

1. FieldBlanks:Theseaccountforallofthesourcesofcontaminationthatmightbeintroduced

toasampleaswellasthoseduetotheimmediatefieldenvironment,suchasallthe

possiblecontaminationsourcesincontainerandequipmentpreparation,transport,

handling,andsamplingmethodology.Fieldblanksaregeneratedunderactualfield

conditionsandaresubjectedtothesameaspectsofsamplecollection,fieldprocessing,

preservation,transport,andlaboratoryhandlingastheenvironmentalsamples.

2. FieldDuplicates/Replicates:Theseaccountforvariabilityinthefieldcollectionand

laboratoryanalysiscombined.

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Travel/bottleblanksmaybecollectedatthediscretionoftheQAO,whenanestablished

procedureischangedorwhenproblemsareidentified:

1. Travel/bottleBlanks:Theseaccountforcontaminantsintroducedduringthetransport

processbetweenthelaboratoryandfieldsite,inadditiontoanycontaminationfromthe

sourcesolutionandcontainer.

2. EquipmentBlanks:Theseaccountforcontaminationintroducedbythefieldsampling

equipmentinadditiontotheabovesources.

Fieldblankswillroutinelybecollectedandanalyzed,astheywillencompassallthepossible

contaminationsourcesincontainerandequipmentpreparation,transport,handling,and

samplingmethodology.Unlessotherwisespecified,goalsforfieldblanksarethesameasforlab

blanks,i.e.,notdetected.Ifproblemsarefoundwithfieldblanks,otherblanksampletypesmay

becollectedinfollow-upsamplingtotryanddeterminethesourceofcontamination.

Fieldblanksforwaterwillbegeneratedunderactualfieldconditionsataminimumfrequency

ofonepersamplingevent(e.g.asetofsamplescollectedbythesamemethodsoverthe

durationofasamplingcruise)orapproximatelyper20samples.Theywillbetreatedinboththe

fieldandlaboratoryproceduresinassimilaramanneraspossibleastheenvironmentalfield

samples.Wholewaterfieldblankswillbetakenbyexposingsamplingcontainersthrougha

simulatedprocessofcollectingsamples.Filedblankcollectionlocationswillbevariedoverthe

courseofastudy.

InstudiesperformedforotherSFEI-ASCprojects,travel/bottleblanksanalyzedusuallyshowed

thattheyarenotasignificantsourceofcontaminationbeyondthatalreadyincludedin

laboratoryblanks,sotravelblanksareseldomcollected.However,ifcontinuedcontamination

isidentifiedinfieldblanks,travelblanksmaybecollectedandanalyzedtoidentifyapotential

source,atthediscretionoftheDeltaRMPQAO.

Fieldduplicates/replicatesofwatersampleswillberoutinelycollectedataminimumfrequency

ofofoneperbatch/samplingeventorper20samplestoevaluatevariabilityincluding

performanceofthesamplingsystemandmethodology.Unlessotherwisespecified,precision

targetsandacceptancecriteriaforfieldduplicates/replicateswillbethesameasthoseforlab

replicates.

4.2. LaboratoryPerformanceMeasurementsforChemicalAnalyses

LaboratoryperformancemeasurementsareincludedintheQAdatareviewtocheckif

measurementqualityobjectivesaremet.ResultsofanalysesofQCsamplesaretobereported

withresultsoffieldsamples.Minimumfrequenciesandtargetperformancerequirementsfor

QCmeasuresofreportedanalytesarespecifiedinTable4.3.

QCmeasurestypicallyusedforevaluationoflaboratoryandfieldsamplingperformanceinclude

thefollowing:

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1. Method(orextraction/preparation)Blanks:samplesofacleanornull(e.g.,empty

container)matrixtakenthroughtheentireanalyticalprocedure,includingpreservatives,

reagents,andequipmentusedinpreparationandquantitationofanalytesinsamples.

2. Field(orequipment/collection)Blanks:samplesofacleanornullmatrixtakenthrough

thesamplingprocedure,thenanalyzedmuchlikeanordinaryfieldsample.

3. SurrogateStandards:analytesintroducedtosamplespriortosampleextractionto

monitorsampleextractionmethodrecoveries.

4. InternalStandards:analytesintroduceafterthelastsample-processingsteppriorto

analysis,tomeasureandcorrectforlossesanderrorsintroducedduringanalysis,with

recoveriesandcorrectionstoreportedvaluesgenerallyreportedforeachsample

individually.

5. MatrixSpikeSamples/Duplicates:fieldsamplestowhichknownamountsoftarget

analytesareadded,indicatingpotentialanalyticalinterferencespresentinfieldsamples

anderrorsorlossesinanalysesnotaccountedforbysurrogatecorrection.

6. CertifiedReferenceMaterials(CRM):CRMsarecreatedorcollectedsamplescontaining

analytesofinterestthathavebeenanalyzedandreportedbymultiplelabsusinga

varietyofmethodstoarriveataconsensus“certified”or“reference”value.Certified

analyteshaveahigherdegreeofcertaintyinreportedvaluesduetoexternalvalidation.

7. LabReferenceMaterials/LaboratoryControlSamples:materialscollectedorcreatedby

alaboratoryasinternalreferencesamples,totrackperformanceacrossbatches.Unlike

CRMs,LRMsandLCSsseldomhaveexternalvalidation(i.e.,measurementbyanother

methodoranotherlab)andarethuslesscertainasmeasuresofaccuracy,butaregood

forday-to-dayindicationofprocesscontrol.

8. InstrumentReplicates:replicateanalysesofextractedmaterialorstandardsthat

measuretheinstrumentalprecision.

9. LaboratoryReplicates:replicatesub-samplesoffieldsamples,standardreference

materials,labreferencematerials,matrixspikesamples,orlaboratorycontrolsamples,

takenthroughthefullanalyticalprocedureincludingalllabprocessescombined.

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Table4.3.Chemical-analyticalQC.

Method Sampletype Matrix Frequency Acceptablelimits

Conventional–Chlorophylla

EPA446.0 LaboratoryBlank Water 1per20orbatch <RL

EPA446.0 CRM Water 1per20orbatch Expectedvalue+/-20%

EPA446.0 LabDuplicate Water 1perbatch

RPD<25%;n/aif

concentrationofeithersample

<RL

EPA446.0 FieldDuplicates Water 5%ofallsamples

RPD<25%;n/aif


<RL

Conventional–DOC

METH011.00or

TM-O-1122-92LaboratoryBlank Water 1per20orbatch <RL

METH011.00or

TM-O-1122-92CRM Water 1per20orbatch Expectedvalue+/-20%

METH011.00or

TM-O-1122-92

Matrix

Spikes/DuplicatesWater 1per20orbatch

Expectedvalue+/-20%;

RPD<25%

METH011.00or

TM-O-1122-92LabDuplicate Water 1per20orbatch

RPD<25%;n/aif


<RL

METH011.00or

TM-O-1122-92FieldDuplicates Water 5%ofallsamples

RPD<25%;n/aif


<RL

Conventional–POC

EPA440 LaboratoryBlank Water 1per20orbatch <MDL

EPA440 CRM Water 1per20orset Expectedvalue+/-10%

EPA440Matrix

Spikes/DuplicatesWater 1per20orbatch Expectedvalue+/-10%

EPA440 LabDuplicate Water 1per20orbatch RPD<10%

EPA440 InstrumentBlank Water 12hours <MDL

EPA440 FieldDuplicates Water 5%ofallsamples RPD<25%

EPA440 FilterBlank Water

1perlotof

filtersorhigher

frequency

<MDL

Conventional–TSS,VSS

SM2540DorTWRI-5-

A1LaboratoryBlank Water 1per20orbatch <RL

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SM2540DorTWRI-5-

A1FieldDuplicates Water 5%ofallsamples

RPD<25%;n/aif


<RL

Pesticides

USGSTM-5-C2 Calibration Water

Ateach

instrumentset

up,major

disruption,and

whenroutine

calibrationcheck

exceedsspecific

controllimits.

Linearregression,r2>0.995

usinga7pointcalibration

curverangingfrom0.01to1

ng/uL

USGSTM-5-C2 CalibrationCheck Water Every6samples. Recovery=75-125%

USGSTM-5-C2 LaboratoryBlanks Water1per20or

batch.<MDL

USGSTM-5-C2Matrix

Spikes/DuplicatesWater 1per20 Recovery70-130%,RPD<25%

USGSTM-5-C2 SurrogateSpikes Water Everysample Recovery=70-130%

USGSTM-5-C2Internal

StandardsWater Everysample Recovery=70-130%

USGSTM-5-C2 FieldBlanks Water 1per20 <MDL

USGSTM-5-C2FieldDuplicate/

ReplicateWater 1per20 RPD<25%

USGS–SIR2012-5026 Calibration Water

Ateach

instrumentset

up,major

disruption,and

whenroutine

calibrationcheck

exceedsspecific

controllimits.

Linearregression,r2>0.995

usingan7pointcalibration

curverangingfrom0.01to1

ng/uL

USGS–SIR2012-5026 CalibrationCheck Water Every6samples. Recovery=75-125%

USGS–SIR2012-5026 LaboratoryBlanks Water1per20or

batch.<MDL

USGS–SIR2012-5026Matrix

Spikes/DuplicatesWater 1per20 Recovery70-130%,RPD<25%

USGS–SIR2012-5026 SurrogateSpikes Water Everysample Recovery=70-130%

USGS–SIR2012-5026Internal

StandardsWater Everysample Recovery=70-130%

USGS–SIR2012-5026 FieldBlanks Water 1per20 <MDL

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USGS–SIR2012-5026FieldDuplicate/

ReplicateWater 1per20 RPD<25%

TraceMetals–Copper(dissolved)

USGSTM-5-B1 LaboratoryBlank Water 1per20orbatch <MDL

USGSTM-5-B1 CRM Water 1per20 Expectedvalue+/-25%

USGSTM-5-B1Matrix

Spikes/DuplicatesWater

1per20orone

batchExpectedvalue+/-25%

USGSTM-5-B1 LabDuplicate Water 1per20 RPD<25%

USGSTM-5-B1 InstrumentBlank Water Every6samples <MDL

USGSTM-5-B1 FieldDuplicates Water 5%ofallsamples RPD<25%

TraceMetals–Mercury

EPA7473 LaboratoryBlank Tissue 1per20orbatch <RL

EPA7473 CRM Tissue 1per20orbatch Expectedvalue+/-25%

EPA7473Matrix

Spikes/DuplicatesTissue 1per20orbatch Expectedvalue+/-25%

EPA7473 LabDuplicate Tissue 1per20

RPD<25%;n/aif


<RL

EPA1631,RevisionE LaboratoryBlank Water1per20or

batch.<RL

EPA1631,RevisionE CRM Water 1per20orbatch Expectedvalue+/-25%

EPA1631,RevisionEMatrix


EPA1631,RevisionE LabDuplicate Water 1per20

RPD<25%;n/aif


<RL

EPA1631,RevisionE FieldDuplicates Water 5%ofallsamples

RPD<25%:n/aif


<RL

EPA1631,RevisionE FieldBlank Water 1per20orbatch <RL

TraceMetals–Mercury,Methyl

EPA1630 LaboratoryBlank Water 1per20orbatch <RL

EPA1630 LCS Water 1per20orbatch Expectedvalue+/-30%

EPA1630Matrix


EPA1630 LabDuplicate Water 1per20 RPD<25%;n/aif


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<RL

EPA1630 FieldDuplicates Water 5%ofallsamples

RPD<25%:n/aif


<RL

EPA1630 FieldBlank Water 1per20orbatch <RL

4.3. LaboratoryQualityControlProceduresforChemicalAnalyses

Priortotheinitialanalysesofsamplesfortheproject,eachlaboratorywilldemonstrate

capabilityandproficiencyformeetingMQOsfortheDeltaRMP.Performance-basedmeasures

forchemicalanalysesconsistoftwobasicelements:initialdemonstrationoflaboratory

capabilityandon-goingdemonstrationofcapabilityduringanalysisofprojectsamples.Initial

demonstrationincludesdocumentationthatsampleanalysescanbeperformedwithinthedata

qualityobjectivesandmethodqualityobjectiveslistedintheQAPP(Tables4.3,4.4,and4.5).

On-goingdemonstrationofcapabilityduringanalysisofprojectsamplesincludeslaboratory

participationinroutineanalyses(e.g.inter-comparisonstudies)toevaluatelaboratory

capabilitiesonacontinualbasistomeetMQOslistedintheQAPP.

4.3.1. LaboratoryQCMeasurements

4.3.1.1 Sensitivity

Inthiscontext,sensitivityreferstothecapabilityofamethodorinstrumenttodetectagiven

analyteatagivenconcentrationandreliablyquantitatetheanalyteatthatconcentration.

Achievingthedesiredsensitivityrequirestheselectionofappropriateanalyticalmethods.The

keymeasurementqualityobjectives(MQOs)forachievingsensitivityarethedesiredReporting

Limit(RL)andMethodDetectionLimit(MDL)foranalytes(Table4.4)andtherangesand

resolutionoflaboratorymeters(Table4.5).AdditionalQCinformationrequiredtoevaluatethe

sensitivityofdataincludelaboratoryormethodblanksand,ifappropriate,instrumentblanks

(Table4.3).

4.3.1.2 Precision

Precisionisthereproducibilityofananalyticalmethodandcanbeevaluatedforanysample

thatisanalyzedinreplicate.Ingeneral,laboratoryreplicatesoffieldsamplesarepreferredas

measuresofprecision,butincaseswhereaveragevaluesforfieldsamplesareexpected(based

onhistoricalorliteratureresults)tofallinanon-quantitativerange,othersamplessuchas

CRMs,LRMs,matrixspikes,orblankspikescanbeanalyzedinreplicatetodetermineprecision.

Ifsamplesotherthanfieldsamplesareusedtoevaluateprecision,targetconcentrationsshould

beatleasthighenoughtobequantitativebutlessthan100timesthoseinfieldsamples,as

precisioninhighconcentrationsamplesisnotlikelyrepresentativeformuchlowerambient

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samples.WhenusingMS/MSD,samplesofasimilarmatrixaremostrelevantandthus

preferredforevaluatingprecision.

Aminimumofonefieldsample(oralternativesampletype,e.g.MS,wheresamplematerialis

insufficientorconcentrationsarelargelynotdetectedinfieldsamples)perbatchofsamples

submittedtothelaboratory(minimumoneper20,or5%,inlargebatches)willbeprocessed

andanalyzedinreplicateforprecision.Previouslyanalyzedmaterial(e.g.fromthesameproject

inprioryears,orfromotherprojects)mayalsobeanalyzedasreplicatestohelpensureresults

inaquantitativerange.Therelativepercentdifference(RPD)amongreplicatesampleswillbe

lessthantheMQOlistedinTable4.3foreachanalyteofinterest.RPDiscalculatedas:

PrecisionmaybeexpressedrelativetoanMQOasap-score:

p=|RPDorRSD|/MQO%

IfresultsforanyanalytedonotmeettheMQOforprecision(p-score>1),calculationsand

instrumentswillbechecked.Repeatanalysesmayberequiredtoconfirmtheresultsand

reduceuncertaintyinthemeasurement.Resultsthatrepeatedlyfailtomeetthecriteria

indicatesampleheterogeneity,unusuallyhighcontaminationofanalytes,orothercausesof

poorlaboratoryprecision.Ifthevariabilityisnotreduced,thelaboratoryisobligatedtohaltthe

analysisofsamples,identifythesourceoftheimprecision,andnotifytheSFEI-ASCProject

ManagerandQAObeforeproceedingwithfurtheranalysis.Insomecaseswhenthecausesof

imprecisioncannotbecorrected(particularlyforlessabundantorlessimportantanalytesina

largegroupreportedbyasingleanalyticalmethod),andwiththeapprovaloftheProject

ManagerandQAO,theresultscanbereportedas-isandflaggedforpoorprecision(p-score>1)

orcensoredifextremelypoor(p-score>2).

4.3.1.3 Accuracy

Theaccuracyoflabmeasurementswillbeevaluatedbasedondataqualitycriteria(Table4.3)

forMS/MSD,CRM,internalstandards,surrogaterecoveries,initialcalibration,andcalibration

checks.

ThepercentrecoveryforMS/MSDiscalculatedusingtheequation

Ifinsufficientsampleisavailable,theanalystcanrunaLCS(LaboratoryControlSample)anda

LCSduplicate.Thecalculationusedisthesame.

%100samples) (replicate Average

samples) replicate(between Difference RPD ×=

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4.3.1.4 Completeness

Completenessisdefinedas“ameasureoftheamountofdatacollectedfromameasurement

processcomparedtotheamountthatwasexpectedtobeobtainedundertheconditionsof

measurement”(StanleyandVerner1985).ThegoaloftheDeltaRMPistoachieve>90%

completenessforallanalyses.

Completenesswillbequantifiedasthetotalnumberofusableresultsdividedbythetotal

numberofsitevisits,aggregatedbyallanalytesofinterest.However,additionalfactorsmaybe

consideredonacase-by-casebasis.Forexample,ananalysismayresultin0%usabledatafora

minorgroupofanalytesandpotentiallynotmeetthecompletenessgoalof90%overallasa

result,butmaystillprovidevaluabledataandmeetthecompletenesscriteriaforallthe

remaininganalyteresultscombined.Incontrast,if>90%completenesscouldnotbeobtained

foragroupofpesticideanalytesthatarethemostabundantinthemajorityofstudiesinthe

literature,itwouldlikelyneedtobeseenasafailurethatneededimmediatecorrection.

4.3.1.5 Contamination

Laboratorymethodblanks(alsocalledextractionblanks,proceduralblanks,orpreparation

blanks)areusedtoassesslaboratorycontaminationduringallstagesofsamplepreparationand

analysis.Forlaboratoryanalyses,atleastonelaboratorymethodblankwillberuninevery

samplebatch.Themethodblankwillbeprocessedthroughtheentireanalyticalprocedureina

manneridenticaltothesamples(i.e.,usingthesamereagentsandequipment).Methodblanks

shouldcontainanalyteconcentrationlessthantheMDL.Amethodblankconcentration>RLfor

anyanalytesofinterestwillrequirecorrectiveaction(e.g.,checkingofreagents,re-cleaning

andre-checkingofequipment)toidentifyandeliminatethesource(s)ofcontaminationbefore

proceedingwithsampleanalysis.Ifeliminatingtheblankcontaminationandreanalysisisnot

possible,resultsforallimpactedanalytesintheanalyticalbatchshallbeflagged.Inaddition,a

detaileddescriptionofthecontaminationsourcesandthestepstakentoidentifyand

eliminate/minimizethemshallbeincludedinthetransmittalletter.Subtractingmethodblank

resultsfromsampleresultsisnotpermitted.

4.3.1.6 Comparability

TheDeltaRMPadherestoEPAguidance,specifiedSOPs,andSWAMP-comparableQA

measures.Therefore,resultscanbecomparedwithotherprojectsandlaboratoriesthatadhere

tothesameorcompatibleprotocolsandQAmeasures.

4.3.1.7 Dataanalysis

Datawillbeanalyzedusingappropriategraphicaltools,spatialanalyses,andstatisticaltestsas

describedintheDeltaRMPCommunicationsPlan.

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Table4.4.SummaryofReportingLimits(RL)andMethodDetectionLimits(MDL)ofDeltaRMP

constituents.

Constituent Matrix Reportinggroup RL MDL Unit

Analyzing

laboratory/

laboratories

Methodused

Oxygen,Dissolved Water FieldParameters 0.5 0.5 mg/L

MPSL(mercury

monitoring),

USGS(pesticide

monitoring)

NationalField

Manualfor

the

Collectionfor

Water-

QualityData,

ChapterA6,

Field

Measure-

ments

pH Water FieldParameters NA NA NA

MPSL(mercury

monitoring),

USGS(pesticide

monitoring)

SpecificConductivity Water FieldParameters 10 10 uS/cm

MPSL(mercury

monitoring),

USGS(pesticide

monitoring)

Temperature Water FieldParameters NA NA NA

MPSL(mercury

monitoring),

USGS(pesticide

monitoring)

Turbidity Water FieldParameters 1 1 FNU USGS

AlkalinityasCaCO3 Water Conventional 12 4 mg/L AHPL SM2320B

AmmoniaasN Water Conventional 0.15 0.05 mg/L AHPLSM4500-

NH3F

Chlorophylla Water Conventional 30 24 µg/L WPCL EPA446.0

HardnessasCaCO3 Water Conventional 6 2 mg/L AHPL SM2340C

DissolvedOrganic

CarbonWater Conventional 0.23 0.23 mg/L

WPCL(mercury

monitoring),

USGS(pesticide

monitoring)

TMO-1122-

92,

METH011.00

ParticulateOrganic

CarbonWater Conventional 0.05 0.05 mg/L USGS EPA440

Copper,dissolved Water TraceMetals 0.8 0.8 ug/L USGS TM-5-B1

Mercury,total Tissue TraceMetals 0.012 0.004µg/gww

MPSL EPA7473

Mercury,total

(unfiltered)Water TraceMetals 0.200 0.200 ng/L MPSL EPA1631E

Mercury,dissolved

(filtered)Water TraceMetals 0.200 0.200 ng/L MPSL EPA1631E

Mercury,Methyl,

total(unfiltered)Water TraceMetals 0.031 0.02 ng/L MPSL EPA1630

Mercury,Methyl,

dissolved(filtered)Water TraceMetals 0.031 0.02 ng/L MPSL EPA1630

ChlorpyrifosOxon Water Degradates 5.0 5.0 ng/L USGS TM-5-C2

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Analyzing

laboratory/

laboratories

Methodused

Dichlorophenyl-3-

methylUrea,3,4-Water Herbicides 3.5 3.5 ng/L USGS

USGS–SIR

2012-5026

DDD(p,p') Water Degradates 6.1 6.1 ng/L USGS TM-5-C2

DDE(p,p') Water Degradates 6.9 6.9 ng/L USGS TM-5-C2

Diazoxon Water Degradates 5.0 5.0 ng/L USGS TM-5-C2

Dichloroaniline,3,4- Water Degradates 3.2 3.2 ng/L USGSUSGS–SIR

2012-5026

Dichloroaniline,3,5- Water Degradates 7.6 7.6 ng/L USGS TM-5-C2

Dichlorophenylurea,

3.,4-Water Degradates 3.4 3.4 ng/L USGS

USGS–SIR

2012-5026

FipronilDesulfinyl Water Degradates 1.6 1.6 ng/L USGS TM-5-C2

FipronilDesulfinyl

AmideWater Degradates 3.2 3.2 ng/L USGS TM-5-C2

FipronilSulfide Water Degradates 1.8 1.8 ng/L USGS TM-5-C2

FipronilSulfone Water Degradates 3.5 3.5 ng/L USGS TM-5-C2

Malaoxon Water Degradates 5.0 5.0 ng/L USGS TM-5-C2

TebupirimphosOxon Water Degradates 2.8 2.8 ng/L USGS TM-5-C2

Acibenzolar-S-methyl Water Fungicides 3.0 3.0 ng/L USGS

Azoxystrobin Water Fungicides 3.1 3.1 ng/L USGS TM-5-C2

Boscalid Water Fungicides 2.8 2.8 ng/L USGS TM-5-C2

Captan Water Fungicides 10.2 10.2 ng/L USGS

Carbendazim Water Fungicides 4.2 4.2 ng/L USGSUSGS–SIR

2012-5026

Chlorothalonil Water Fungicides 4.1 4.1 ng/L USGS TM-5-C2

Cyazofamid Water Fungicides 4.1 4.1 ng/L USGSUSGS–SIR

2012-5026

Cymoxanil Water Fungicides 3.9 3.9 ng/L USGSUSGS–SIR

2012-5026

Cyproconazole Water Fungicides 4.7 4.7 ng/L USGS TM-5-C2

Cyprodinil Water Fungicides 7.4 7.4 ng/L USGS TM-5-C2

Desthio-

ProthioconazoleWater Fungicides 3.0 3.0 ng/L USGS

USGS–SIR

2012-5026

Difenoconazole Water Fungicides 10.5 10.5 ng/L USGS TM-5-C2

Dimethomorph Water Fungicides 6.0 6.0 ng/L USGS TM-5-C2

Ethaboxam Water Fungicides 3.8 3.8 ng/L USGSUSGS–SIR

2012-5026

Famoxadone Water Fungicides 2.5 2.5 ng/L USGS TM-5-C2

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Analyzing

laboratory/

laboratories

Methodused

Fenamidone Water Fungicides 5.1 5.1 ng/L USGS TM-5-C2

Fenarimol Water Fungicides 6.5 6.5 ng/L USGS TM-5-C2

Fenbuconazole Water Fungicides 5.2 5.2 ng/L USGS TM-5-C2

Fenhexamid Water Fungicides 7.6 7.6 ng/L USGS TM-5-C2

Fluazinam Water Fungicides 4.4 4.4 ng/L USGS TM-5-C2

Fludioxonil Water Fungicides 7.3 7.3 ng/L USGS TM-5-C2

Fluopicolide Water Fungicides 3.9 3.9 ng/L USGS TM-5-C2

Fluoxastrobin Water Fungicides 9.5 9.5 ng/L USGS TM-5-C2

Flusilazole Water Fungicides 4.5 4.5 ng/L USGS TM-5-C2

Flutolanil Water Fungicides 4.4 4.4 ng/L USGS TM-5-C2

Flutriafol Water Fungicides 4.2 4.2 ng/L USGS TM-5-C2

Fluxapyroxad Water Fungicides 4.8 4.8 ng/L USGS TM-5-C2

Imazalil Water Fungicides 10.5 10.5 ng/L USGS TM-5-C2

Ipconazole Water Fungicides -- -- ng/L USGS TM-5-C2

Iprodione Water Fungicides 4.4 4.4 ng/L USGS TM-5-C2

Kresoxim-methyl Water Fungicides 4.0 4.0 ng/L USGS TM-5-C2

Mandipropamid Water Fungicides 3.3 3.3 ng/L USGSUSGS–SIR

2012-5026

Metalaxyl Water Fungicides 5.1 5.1 ng/L USGS TM-5-C2

Metconazole Water Fungicides 5.2 5.2 ng/L USGS TM-5-C2

Myclobutanil Water Fungicides 6.0 6.0 ng/L USGS TM-5-C2

Paclobutrazol Water Fungicides 6.2 6.2 ng/L USGS TM-5-C2

PCNB Water Organochlorines 3.1 3.1 ng/L USGS TM-5-C2

Picoxystrobin Water Fungicides 4.2 4.2 ng/L USGS TM-5-C2

Propiconazole Water Fungicides 5.0 5.0 ng/L USGS TM-5-C2

Pyraclostrobin Water Fungicides 2.9 2.9 ng/L USGS TM-5-C2

Pyrimethanil Water Fungicides 4.1 4.1 ng/L USGS TM-5-C2

Quinoxyfen Water Fungicides 3.3 3.3 ng/L USGS TM-5-C2

Sedaxane Water Fungicides -- -- ng/L USGS TM-5-C2

Tebuconazole Water Fungicides 3.7 3.7 ng/L USGS TM-5-C2

Tetraconazole Water Fungicides 5.6 5.6 ng/L USGS TM-5-C2

Thiabendazole Water Fungicides 3.6 3.6 ng/L USGSUSGS–SIR

2012-5026

Triadimefon Water Fungicides 8.9 8.9 ng/L USGS TM-5-C2

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Analyzing

laboratory/

laboratories

Methodused

Triadimenol Water Fungicides 8.0 8.0 ng/L USGS TM-5-C2

Trifloxystrobin Water Fungicides 4.7 4.7 ng/L USGS TM-5-C2

Triflumizole Water Fungicides 6.1 6.1 ng/L USGS TM-5-C2

Triticonazole Water Fungicides 6.9 6.9 ng/L USGS TM-5-C2

Zoxamide Water Fungicides 3.5 3.5 ng/L USGS TM-5-C2

Alachlor Water Herbicides 1.7 1.7 ng/L USGS TM-5-C2

Atrazine Water Herbicides 2.3 2.3 ng/L USGS TM-5-C2

Benefin Water Herbicides 2.0 2.0 ng/L USGS TM-5-C2

Butralin Water Herbicides 2.6 2.6 ng/L USGS TM-5-C2

Butylate Water Herbicides 1.8 1.8 ng/L USGS TM-5-C2

Clomazone Water Herbicides 2.5 2.5 ng/L USGS TM-5-C2

Cycloate Water Herbicides 1.1 1.1 ng/L USGS TM-5-C2

Cyhalofop-butyl Water Herbicides 1.9 1.9 ng/L USGS TM-5-C2

Dacthal Water Herbicides 2.0 2.0 ng/L USGS TM-5-C2

Diuron Water Herbicides 3.2 3.2 ng/L USGSUSGS–SIR

2012-5026

Dithiopyr Water Herbicides 1.6 1.6 ng/L USGS TM-5-C2

EPTC Water Herbicides 1.5 1.5 ng/L USGS TM-5-C2

Ethalfluralin Water Herbicides 3.0 3.0 ng/L USGS TM-5-C2

Flufenacet Water Herbicides 4.7 4.7 ng/L USGS TM-5-C2

Fluridone Water Herbicides 3.7 3.7 ng/L USGSUSGS–SIR

2012-5026

Hexazinone Water Herbicides 8.4 8.4 ng/L USGS TM-5-C2

Metolachlor Water Herbicides 1.5 1.5 ng/L USGS TM-5-C2

Molinate Water Herbicides 3.2 3.2 ng/L USGS TM-5-C2

Napropamide Water Herbicides 8.2 8.2 ng/L USGS TM-5-C2

Novaluron Water Herbicides 2.9 2.9 ng/L USGS TM-5-C2

Oryzalin Water Herbicides 5.0 5.0 ng/L USGSUSGS–SIR

2012-5026

Oxadiazon Water Herbicides 2.1 2.1 ng/L USGS TM-5-C2

Oxyfluorfen Water Herbicides 3.1 3.1 ng/L USGS TM-5-C2

Pebulate Water Herbicides 2.3 2.3 ng/L USGS TM-5-C2

Pendimethalin Water Herbicides 2.3 2.3 ng/L USGS TM-5-C2

Penoxsulam Water Herbicides 3.5 3.5 ng/L USGSUSGS–SIR

2012-5026

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Analyzing

laboratory/

laboratories

Methodused

Prodiamine Water Herbicides 5.2 5.2 ng/L USGS TM-5-C2

Prometon Water Herbicides 2.5 2.5 ng/L USGS TM-5-C2

Prometryn Water Herbicides 1.8 1.8 ng/L USGS TM-5-C2

Propanil Water Herbicides 10.1 10.1 ng/L USGS TM-5-C2

Pronamide Water Herbicides 5.0 5.0 ng/L USGS TM-5-C2

Simazine Water Herbicides 5.0 5.0 ng/L USGS TM-5-C2

Thiazopyr Water Herbicides 4.1 4.1 ng/L USGS TM-5-C2

Thiobencarb Water Herbicides 1.9 1.9 ng/L USGS TM-5-C2

Triallate Water Herbicides 2.4 2.4 ng/L USGS TM-5-C2

Tributyl

Phosphorotrithioate,

S,S,S-

Water Herbicides 3.1 3.1 ng/L USGS TM-5-C2

Trifluralin Water Herbicides 2.1 2.1 ng/L USGS TM-5-C2

Acetamiprid Water Insecticides 3.3 3.3 ng/L USGSUSGS–SIR

2012-5026

Allethrin Water Insecticides 4.1 4.1 ng/L USGS TM-5-C2

Bifenthrin Water Insecticides 4.7 4.7 ng/L USGS TM-5-C2

Carbaryl Water Insecticides 6.5 6.5 ng/L USGS TM-5-C2

Carbofuran Water Insecticides 3.1 3.1 ng/L USGS TM-5-C2

Chlorantraniliprole Water Insecticides 4.0 4.0 ng/L USGSUSGS–SIR

2012-5026

Chlorpyrifos Water Insecticides 2.1 2.1 ng/L USGS TM-5-C2

Clothianidin Water Insecticides 3.9 3.9 ng/L USGSUSGS–SIR

2012-5026

Coumaphos Water Insecticides 3.1 3.1 ng/L USGS TM-5-C2

Cyantraniliprole Water Insecticides 4.2 4.2 ng/L USGSUSGS–SIR

2012-5026

Cyfluthrin,total Water Insecticides 5.2 5.2 ng/L USGS TM-5-C2

Cyhalothrin,total Water Insecticides 4.5 4.5 ng/L USGS TM-5-C2

Cypermethrin,total Water Insecticides 5.6 5.6 ng/L USGS TM-5-C2

DDT(p,p') Water Insecticides 3.5 3.5 ng/L USGS TM-5-C2

Deltamethrin Water Insecticides 3.5 3.5 ng/L USGS TM-5-C2

Diazinon Water Insecticides 0.9 0.9 ng/L USGS TM-5-C2

Dinotefuran Water Insecticides 4.5 4.5 ng/L USGSUSGS–SIR

2012-5026

Esfenvalerate Water Insecticides 3.9 3.9 ng/L USGS TM-5-C2

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Analyzing

laboratory/

laboratories

Methodused

Ethofenprox Water Insecticides 2.2 2.2 ng/L USGS TM-5-C2

Fenpropathrin Water Insecticides 4.1 4.1 ng/L USGS TM-5-C2

Fenpyroximate Water Insecticides 5.2 5.2 ng/L USGS TM-5-C2

Fenthion Water Insecticides 5.5 5.5 ng/L USGS TM-5-C2

Fipronil Water Insecticides 2.9 2.9 ng/L USGS TM-5-C2

Flonicamid Water Insecticides 3.4 3.4 ng/L USGSUSGS–SIR

2012-5026

Imidacloprid Water Insecticides 3.8 3.8 ng/L USGSUSGS–SIR

2012-5026

Indoxacarb Water Insecticides 4.9 4.9 ng/L USGS TM-5-C2

Malathion Water Insecticides 3.7 3.7 ng/L USGS TM-5-C2

Methoprene Water Insecticides 6.4 6.4 ng/L USGS TM-5-C2

Methoxyfenozide Water Insecticides 2.7 2.7 ng/L USGSUSGS–SIR

2012-5026

Methidathion Water Insecticides 7.2 7.2 ng/L USGS TM-5-C2

Parathion,Methyl Water Insecticides 3.4 3.4 ng/L USGS TM-5-C2

Pentachloroanisole Water Insecticides 6.5 6.5 ng/L USGS TM-5-C2

Permethrin,total Water Insecticides 3.4 3.4 ng/L USGS TM-5-C2

Phenothrin Water Insecticides 5.1 5.1 ng/L USGS TM-5-C2

Phosmet Water Insecticides 4.4 4.4 ng/L USGS TM-5-C2

Propargite Water Insecticides 6.1 6.1 ng/L USGS TM-5-C2

Pyridaben Water Insecticides 5.4 5.4 ng/L USGS TM-5-C2

Resmethrin Water Insecticides 5.7 5.7 ng/L USGS TM-5-C2

Tebupirimfos Water Insecticides 1.9 1.9 ng/L USGS TM-5-C2

Tefluthrin Water Insecticides 4.2 4.2 ng/L USGS TM-5-C2

Tetradifon Water Insecticides 3.8 3.8 ng/L USGS TM-5-C2

Tetramethrin Water Insecticides 2.9 2.9 ng/L USGS TM-5-C2

Thiacloprid Water Insecticides 3.2 3.2 ng/L USGSUSGS–SIR

2012-5026

Thiamethoxam Water Insecticides 3.4 3.4 ng/L USGSUSGS–SIR

2012-5026

Tolfenpyrad Water Insecticides 2.9 2.9 ng/L USGSUSGS–SIR

2012-5026

Piperonylbutoxide Water Synergists 2.3 2.3 ng/L USGS TM-5-C2

Flumetralin WaterPlantGrowth

Regulator5.8 5.8 ng/L USGS TM-5-C2

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--=MDL/RLnotyetdetermined.

Table4.5.Summaryofinstrumentrangesandresolutionforlaboratorymeters.

Constituent MatrixReporting

group

Instrument

RangeResolution Unit

Analyzing

laboratory

Instrument

used

Oxygen,

DissolvedWater Conventional 0to20 0.1 mg/L AHPL YSI58

pH Water Conventional 1to16 0.01 NA AHPL Beckman255

Specific

ConductivityWater Conventional

0to499.9

0to4999

0.1

1uS/cm AHPL YSI30

Temperature Water Conventional -200to100 0.1 °C AHPLOnset

HOBOware

4.3.2. LaboratoryQCSamples

DatafromthelaboratoryshouldincludeattheleastthefollowingQCdata:

1. SurrogateRecovery(forallfieldandQCsamples,ifapplicable)

2. MethodBlank

3. MatrixSpikeRecovery(ifapplicable)

4. Replicateprecision(field,CRM,matrixspike,blankmatrixspikesamples)

5. Certified/LabReferenceMaterial(CRM/LRM)Recovery(ifapplicable)

Surrogatespikesshouldbeincludedinallsampleswhereappropriatefortheanalysis.Although

surrogatespikerecoveriescanbeusedtoestimateandcorrectforlossesofthetargetanalytes

intheanalyticalprocess,unusuallyloworhighrecoveriesreflectanalyticalissuesthatarenot

overcomesimplybysurrogatecorrection,becauseatlowrecoveries,surrogatecorrection

factorsbecomeinverselylarger.Itisgenerallylefttotheprofessionaljudgmentofthelab’s

QAOtosetappropriatecontrol/acceptancelimitsandcorrectiveactionsforsurrogate

recoveries.

Methodblanksshouldberunataminimumfrequencyofoneperanalyticalbatch(foranalytical

batchesconsistingofupto20fieldsamples)orper20(field)samplesforlargeranalytical

batches.Resultsforlaboratorymethodblanks,combinedwiththoseforfieldblanks,canhelp

identifywhetherprobablecausesofsamplecontaminationoriginatedinthefieldorin

laboratoryanalyses.Ifbothfieldandlabmethodblankshavesimilarlevelsofcontamination,it

islikelycausedprimarilyinlabprocedures.Iffieldblankshavehighercontamination,sample

collectionmethodsarelikelythecause.Rawresultsformethodblanksshouldbereported.

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Matrixspikes(MS)shouldberunataminimumfrequencyofoneperbatchorper20samples.

Matrixspikeresultsaretobereported,alongwiththeexpectedresult(unspikedsample

concentration+spikeconcentration),andarecoveryestimate.Thespikingconcentrations

shouldbehighenoughtoproduceanexpectedresultsufficientlyovertheanalyticalvariability

inquantifyingtheunspikedsampletoquantifyrecovery(atleast~3timestheunspikedresult),

butalsolowenoughtobearelevantaccuracyindicatorintheconcentrationrangeoffield

samples(below100xandpreferablynearer10xtheunspikedresult).Incaseswhereanalytes

aremostlynotdetectedinunspikedsamples,aconcentrationrangeofthatmagnitude(10-

100x)overtheMDLmaybeappropriatetouseinstead.

Precisioncanbedeterminedwithallsampletypesanalyzedandreportedinreplicate.Lab

replicates(splitandanalyzedinthelaboratory)offieldsamplesaregenerallythepreferred

indicatorofprecisionfortypicalfieldsamples,asthetargetanalyteconcentrationrange,

matrix,andinterferencesaremostsimilartopreviousanalyzedsamplesorsamplesfrom

nearbysites.However,sometimesfieldsampleconcentrationsarebelowdetectionlimitsfor

manyanalytes,soreplicateresultsonCRMs,LRMs,MS/MSDs,orblankspikes(LCSs)maybe

neededtosupplementandobtainquantitativeprecisionestimates.Thesealternativesample

types,inparticularblankspikes(LCSs),shouldnotserveastheprimaryorexclusiveindicatorof

measurementprecisionwithoutpriorapprovalbytheProjectManagerandQAO.LCSsareoften

createdfromacleanlaboratorymatrix,sotheyarelikelynotrepresentativeofthe

measurementprecisionroutinelyachievableinmorecomplexmatricesofrealfield-originated

samples.RPDsshouldbecalculatedasdescribedpreviouslyandreportedforallsamples

analyzedinreplicate.

Certifiedreferencematerial(CRM)orotherexternallyestablishedperformancetestingsamples

shouldberunataminimumfrequencyofoneperanalyticalbatch(foranalyticalbatches

consistingofupto20fieldsamples)orper20(field)samplesforlargeranalyticalbatches.

Resultsshouldbereportedalongwiththeexpectedvaluesandrecoveries(as%oftheexpected

value),whereavailablefortargetanalytesinappropriatematrices.Insomecases,nowidely

availablereferencematerialshavebeenestablishedandlaboratoriesmaintaininternallab

referencematerials(LRM)totracktherelativeinternalaccuracyofananalyticalmethod.CRMs

arelikelythemostrobustindicatorsofmeasurementaccuracy,givenrequirementsfor

consensusamonglabsaswellasvalidationthroughdifferentmethodsofmeasurement.

ReferencevaluesforCRMsorinternalLRMs,althoughlessrigorous(fewerlabsinconsensus,or

onlyoneanalyticalmethodprovided),provideatleastsomeindicatorofmeasurement

accuracy.Althoughpoorrecoveriesontheseuncertifiedvaluesmaybeusedtoflagpotentially

unreliabledataforuseindataanalysesanddecision-making,theyshouldnotbeusedtociteor

sanctionalabfor“failing”tomeetMQOrequirements.

Table4.6listsrecoverysurrogatestandardsusedforpesticideanalysesandassociated

measurementqualityobjectives.

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Table4.6. Recoverysurrogatestandardsusedforpesticideanalysesandassociatedmeasurementqualityobjectives.

Recoverysurrogate

standardMatrix Method

Acceptablelimits

(%recovery)13C3-atrazine Water TM-5-C2 70-130%

Di-N-propyl-d14trifluralin Water TM-5-C2 70-130%

Monuron Water USGS–SIR2012-5026 70-130%

Imidacloprid-d4 Water USGS–SIR2012-5026 70-130%

4.4. DataQualityIndicatorsandTestAcceptabilityCriteriaforToxicityTestingandAssociatedWaterQualityMeasurements

InthecontextoftheRMP,toxicitymonitoringshouldbeviewedprimarilyasasetoftoolsto

helpidentifypesticidesthatarecausingsignificantaquatictoxicityintheDelta.Becausetoxicity

testingisanintegrativetool,itcandetermineeffectsofmultipleconstituentsconcurrently,and

canbemorecost-effectivethanchemicalanalysisofindividualconstituents.

ToxicityIdentificationEvaluations(TIEs)areplannedforDeltaRMPsampleswherethereis>50

percenteffectwithin96hoursofthetestperiod.TIEsshouldbeinitiatedwithin48hoursofthe

observationoftheTIEtriggerbeingmetintheinitialsamplescreening(seealsoSection8.3).

TheprimarygoalofDeltaRMPTIEtestingistoidentifywhetherpesticidesarecausingor

contributingtoobservedtoxicity,andifso,whichpesticides(ordegradates,oranyoftheinert

ingredientsintheformulatedproduct)arethedrivers.Asecondarygoalistoidentifyother

factors(i.e.,waterqualityconditionsorothertoxicants)contributingtoreducedsurvival,

growth,orreproduction.

Dataqualityindicatorsfortoxicitytestingandassociatedwaterqualitymeasurementsare

outlinedinTable4.7,andtestacceptabilitycriteriaaresummarizedinTable4.8.Testresults

willberejectedwhenmeasurementqualityobjectivesandtestacceptabilitycriteriaarenot

met.However,thesamplemayberetestedandqualifiedwithanextendedholdingtimeifSFEI-

ASCandtheDeltaRMPSCpermit.Toxicitydatawillbequalifiedininstanceswheredatadoes

notmeetaccuracyandprecisioncriteriabelow.

Thewaterqualitymeasurementsspecificallycoupledtotoxicitytestsareintendedtohelp

interprettoxicitytestdata.QualitycontrolpracticesandMQOsparallelthoseusedforfield

meterinstrumentation.Metersarecalibratedatthebeginningofeachdayandcalibration

checksareperformedwhenmeasurementsforthedayexceed20readingsforeachmeter.

MetersarerecalibratedwhendriftexceedstheMQOforaccuracyinTable4.7below.Field

duplicatesareexpectedtofallwithintheprecisionMQOsbelowanddataarequalifiedin

instanceswhentheseareexceeded.

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Table4.7.Measurementqualityobjectivesfortoxicitytestingandassociatedwaterquality

measurements.

ToxicityTestingLaboratoryAnalysis

Parameter Accuracy Precision Completeness

pH ±0.2 ±0.5pHunits 90%

Specific

Conductance ±0.5% ±10% 90%

Temperature ±0.1 ±10% 90%

DissolvedOxygen ±0.2 ±10% 90%

Ammonia ±0.5% ±10% 90%

Hardness

Standard

ReferenceMaterial

(SRM)within80to

120%recovery

RPD<20% 90%

Alkalinity SRMwithin80to

120%recovery RPD<20% 90%

ToxicityTesting N/A

Referencetoxicant

performance,based

oncumulativecontrol

charts:LC50and/or

EC25within2standard

deviationsofthe

runningmeanfromat

least20datapoints

90%

Table4.8.Summaryoftestacceptabilitycriteria.

Species Duration Endpoint(s) Method Testacceptabilitycriteria

S.capricornutum 4-days Growth UCDAHPLSOP1-1 Meancelldensityofat

least2X105cells/mLinthe

controls;andvariability

(CV%)amongcontrol

replicates≤20%

C.dubia

6-8days Survival,

Reproduction

UCDAHPLSOP1-2 >80%survivalofallcontrol

organismsandanaverage

of15ormoreyoungper

survivingfemaleinthe

controlsolutions.60%of

survivingcontrolfemales

mustproducethreebroods

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Species Duration Endpoint(s) Method Testacceptabilitycriteria

(required)

H.azteca10

4days Survival UCDAHPLSOP1-6 >90%survival

P.promelas 7-days Survival,

Biomass

UCDAHPLSOP1-3 >80%survivalincontrols;

averagedryweightper

survivingorganismin

controlchambersequalsor

exceeds0.25mg/surviving

fish

4.4.1. QualityAssuranceActivities

Alltoxicitytestprotocolsarebasedonmethodsoutlinedin“SummaryofTestConditionsand

TestAcceptabilityCriteria”tablesinEPA(2002aandb).Deviationsfromprotocolsmustbe

reportedtotheQAO,theprojectmanager,andininterimandfinalreports,dependingonthe

degreeofdepartureandtheobjectiveofthetest.Deviationsmayormaynotinvalidateatest

result.Beforerejectingoracceptingatestresultasvalid,thedegreeofthedeviationandthe

potentialorobservedimpactofthedeviationonthetestresultwillbeevaluated.

Predetermineddeviationsinprotocolssuchasalternatetestproceduresoruseofsurrogate

speciesmustbediscussedandapprovedpriortotestinitiation.Dataqualityindicatorsforthis

projectwillconsistofthefollowing:

Variabilityandbias

TheDeltaRMPhasseveralmechanismstoensurethatvariabilityandbiasareminimized

throughouttheproject.Techniciansaretrainedaccordingtostandardlaboratoryprotocolsto

ensurethatsamplesarecollectedandanalyzedinaconsistentmanner.Referencetoxicant

testswillbeconductedthroughouttheprojecttoensurethatorganismsensitivityremains

constantovertime.Therelativepercentdifferencebetweenfieldduplicatewaterquality

measurementswillbemeasuredtoensuretechniciansareanalyzingsamplesinaconsistent

manner.AmbientsamplesaretrackedandlabelledwithsitecodesandSampleIDnumbers

ratherthanassociatedwithwaterbodynamestoreducetechnicianbiasofsamples,and

laboratorytestreplicatesareinitiatedwithtestorganismsrandomlytoreducebiasoforganism

health.

10InclusionofHyalellawatertoxicitytestingispendingafinaldecisionbytheSC.

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Testsensitivity

TheDeltaRMPutilizestherequiredminimumnumberofreplicatesspecifiedbyEPAtoensure

adequatetestsensitivity.Testsensitivityisalsoevaluatedthroughreferencetoxicanttesting,

whichmeasuresboththelaboratoryperformanceandtherelativesensitivityofthetestspecies

overtime.

Positivecontroltests.Referencetoxicanttestswillbeperformedconcurrentlyforeachevent

forH.aztecaandP.promelas.ReferencetoxicanttestsforC.dubiaandS.capricornutumwillbe

performedmonthlyaccordingtoEPAtestmethodforin-housecultures.

Zincchloride(ZnCl2)willbeusedasthereferencetoxicantforS.capricornutum;sodium

chloride(NaCl)willbethereferencetoxicantusedfortheC.dubia,H.azteca,andP.promelasspecies.TheLC50(theconcentrationatwhich50%oftestorganismsexhibitmortality)for

survivalorEC25sublethalendpoint(theconcentrationatwhich25%oftestorganismsexhibita

response)foreachreferencetoxicanttestiscomparedtothelaboratory’srunningmeanto

ascertainwhetheritfallswithintheacceptablerange.EPAtestmethodmanualsincludethe

addedcautionthatreferencetoxicanttestresultsshouldnotbeusedasadefactocriterionforrejectionofindividualreceivingwatertests.Referencetoxicanttestsdoprovideinformationon

trendsinorganismsensitivityandlaboratoryperformancethatcanbeusefulinevaluatingand

interpretingtoxicitytestresults.Forthisreason,EPAhasrecommendedevaluatingthe

followingelementsofreferencetoxicanttestresultsinthereviewofthereceivingwatertest

data:thedegreetowhichthereferencetoxicanttestsresultisoutsideofcontrolchartlimits:

thewidthofthelimits;thedirectionofadeviation(towardincreasedtestorganismsensitivity

ortowarddecreasedtestorganismsensitivity);thetestconditionsofboththeambientorsite

waterandthereferencetoxicanttests;andtheobjectiveofthetest.TheEPAacceptablerange

iswithintwostandarddeviationsoftherunningmean.IftheLC50and/orEC25falloutsideofthe

upperandlowertwostandarddeviationlimits,testorganismsensitivitymaybeatypicaland

resultsofambientsampletoxicitytestsconductednearesttothetimewhenthereference

toxicanttestwasperformedwillbequalifiedaseithermoresensitiveorlesssensitivethan

usual.SeeEPA2002aformoreinformation.

Precision

Precisionisthedegreetowhichindependentanalysesofagivensampleagreewithone

another.Itisthereproducibility,consistencyandrepeatabilityofresults.UCDAHPLassesses

precisionthroughfieldduplicates.Afieldduplicateisasecondsamplecollectedinaseparate

container,immediatelyaftertheinitial/primarytestsample.Fieldduplicatesaretested

concurrentlywithitsprimarysampleandtheresultsareevaluatedtodetermineprecisionof

fieldandlaboratorystaff.Fieldduplicatesamplesareinagreementwhentheyarebotheither

statisticallysimilar,orstatisticallydifferentfromthecontrol.Fieldduplicateswillbeconducted

atarateof5%oftotalprojectsamplecount.

Therelativepercentdifference(RPD)betweenduplicatesiscalculatedonwaterchemistry

measurementsusingthefollowingformula:

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Accuracy

Accuracyoftoxicitytestscannotbedirectlymeasuredbecauseofthelackofdatatosupporta

standardorganismresponseagainstcomparabletestresults.However,inferencescanbemade

regardingaccuracyfromreferencetoxicanttestsinordertoassessthesensitivityofthe

organismsinaknownconcentrationoftoxicant,andtodeterminethattheorganisms’response

iswithinacceptablelimits.Accuracyofinstrumentswillbeevaluatedusingtheformulafor

accuracylistedinAppendixAoftheSWAMPQAPrPandwillfollowtheMQOslistedinTable4-

7.

Completeness

TheDeltaRMPstrivesforaminimumof90%completionofdata.Fortoxicitytests,

completenessisdefinedbythetotalnumberofsamplesthatmetTestAcceptabilityCriteriafor

eachspeciesdividedbythetotalnumberofuseablesamplessubmittedtothelaboratoryfor

eachspecies.Anindividualsamplemaynotbeusableifitsconductivityiswellaboveorbelow

conductivitiestypicallyfoundinfreshwater.Theseconductivitythresholdsaredifferentfor

eachspecies.Toxicitycompletenessisassessedbythenumberofuseableresultsdividedbythe

totalnumberofsamplescollected.

Forwaterqualitydataassociatedwithtoxicitytesting,datawillbeconsideredcompletewhen

eachsampleismeasuredwithinasamplebatchthatmeetstheaccuracyrequirementsforthe

referencematerial(hardness,alkalinityandtotalammonia),ormeterdrift(DO,ECandpH)is

withinacceptablelimits.

Representativeness

Intermsoflaboratorytoxicitytestingofambientsamples,representativenessreferstothe

degreetowhichdataaccuratelyreflectthepresenceorabsenceoftoxiccontaminantsinthe

environmentatthesiteswheresamplesarecollected.Locationofsamplingsites,sample

preservationandappropriatespeciesselectionareimportantconsiderationsfor

representativeness.

Comparability

TheDeltaRMPdocumentsadheretoEPAtestmethods,SOPsandQAmeasuresspecifiedinthe

QAPP,andacceptablereferencetoxicanttestresults.Therefore,resultscanbecomparedwith

otherprojectsandlaboratoriesthatadheretothesameEPAprotocolsandQAmeasures.

[ ][ ]

100*2121*2⎟⎟⎠

⎞⎜⎜⎝

⎛

+

−=

DupDupDupDup

RPD

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Dataanalysis

Toxicitytestswillbeconductedusingasingle-concentrationtestdesign.Datafromlaboratory

toxicitytestswillbeanalyzedaccordingtotheEPAstandardsingleconcentrationstatistical

protocols(EPA,2002,AppendixH).TheEPAmethodofdataanalysisinvolvesthecomparisonof

eachsampletoonecontrol(laboratorycontroloraconductivitycontrol),andcalculatesthe

testresultaccordingtothestandardizedstatisticalapproachusedinaquatictoxicology.

ComprehensiveEnvironmentalToxicityInformationSystem™ (CETIS;TidepoolScientific,McKinleyville,CA,USA)toxicitydataanalysisanddatabase)softwareapplicationwillbeusedto

calculateEffectConcentrationandLethalConcentrationvalues(EC25forsublethalendpoints

andLC50forsurvivalendpoints)forreferencetoxicanttests.

4.4.2. QualityControl

Table4.9providesasummaryofQCmeasuresandalsoliststhetoxicityendpointsthattriggera

TIE.Table4.10providesMQOsrelatedtotoxicitytesting.Section8.3CorrectiveActions

providesinformationonqualitycontrolactionswhenacceptancelimits(i.e.“actionlimits”)are

exceeded.

Table4.9. QualityControlMeasuresfortoxicitytesting.

Method Analyte/Test Matrix

HoldingTime/

Preservation AcceptabilityLimit

SM2320B;

UCDAHPSOP6-5Alkalinity(asCaCO3) Water

14days;

Coolto0to6°CNA

SM4500-NH3F;

UCDAHPSOP6-3Ammonia Water

48hours,28daysif

acidified

Coolto0to6°C;

samplesmaybe

preservedwith2

mLofH2SO4/L

<5mg/L

SM2510B;

UCDAHPSOP8-7Conductivity Water

28days;

Coolto0to6°C;if

analysisisnot

completedwithin

24hoursofsample

collection,sample

shouldbefiltered

througha0.45

micronfilterand

storedat≤6°C

Additionofconductivity

controlsintestbatches:

>1500µS/cmforS.capricornutum,<100or>

1900µS/cmforC.dubia,<100or>1900µS/cmforP.promelas;and<100mS/cm

or>10,000µS/cmforH.azteca.

SM4500OG;

UCDAHPSOP8-9DissolvedOxygen Water

48hours;

Coolto0to6°C;

add1gFAS

crystalsperliterif

residualchlorineis

<8.6mg/L(saturation)(H.azteca<8.9[saturation]mg/L)

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Method Analyte/Test Matrix

HoldingTime/

Preservation AcceptabilityLimit

present

SM2340C;

UCDAHPSOP6-1Hardness Water

14days;

Coolto0to6°CNA

SM4500H+B;

UCDAHPSOP8-8pH Water NA 6-9

SM2550B Temperature Water NA

25+1°C

(H.azteca23+1°C)Testtemperaturesmustnot

deviate(i.e.,maximum

minusminimum

temperature)bymorethan

3°Cduringthetest

EPA1003.0 S.capricornutum Water48hours;

Coolto0to6°C

LaboratoryControlmust

meettestacceptability

criterialistedinTable4.8

TIEtrigger:>50%reduction

incellgrowth

EPA1002.0 C.dubia Water36hours;

Coolto0to6°C




TIEtrigger:>50%mortalityin

96hours

EPA1000.0 P.promelas Water36hours;

Coolto0to6°C




TIEtrigger:>50%mortalityin

96hours

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Table4.10. MeasurementQualityObjectivesfortoxicitytesting.

Method Analyte/Test QualityControl Frequencyof

Analysis

ControlLimit/

MQO

SM2320B;

UCDAHPSOP6-5

Alkalinity(as

CaCO3)

Reference

Material

Peranalytical

batch±10%

Laboratory

Blank

Peranalytical

batch<12mg/L

SM4500-NH3F;

UCDAHPSOP6-3Ammonia

Reference

Material

Peranalytical

batch±10%

Laboratory

Blank

Peranalytical

batch<0.15mg/L

SM2510B;

UCDAHPSOP8-7Conductivity

Calibration

Standard:

InternalCell

Constant

Peranalytical

batch

(atleastoncedaily)

Permanufacturer’s

specifications

SM4500OG;

UCDAHPSOP8-9

Dissolved

Oxygen

Oxygen

Saturated

Waterat25°C

Peranalytical

batch

(atleastoncedaily)

Peranalyticalmethod

ormanufacturer’s

specifications

SM2340C;

UCDAHPSOP6-1Hardness

Reference

Material

Peranalytical

batch±10%

Laboratory

Blank

Peranalytical

batch<6mg/L

SM4500H+B;

UCDAHPSOP8-8pH

Calibration

Standard

Peranalytical

batch

(atleastoncedaily)

Permanufacturer’s

specifications

SM2550B TemperatureCertified

ThermometerOnceannually ±0.5°C

ChronicToxicity

TestingAllspecies

Laboratory

ControlWater

Peranalytical

batch

Mustmeetalltest

acceptabilitycriteriafor

thespeciesofinterest

(seeTable4.8)

Conductivity

ControlWater

Peranalytical

batchwhen

conductivity

parametersare

aboveorbelowthe

species’tolerance

(seeTable4.9a)

FollowEPAguidanceon

interpretingdata

Additional

ControlWater

Peranalytical

batchwhen

manipulationsare

performedonone

Nostatisticaldifference

betweenthelaboratory

controlwaterandeach

adidionalcontrolwater

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Method Analyte/Test QualityControl Frequencyof

Analysis

ControlLimit/

MQO

ormoreambient

samplewithineach

analyiticalbatch

withinananalytical

batch

Reference

ToxicantTests

Monthlyforin-

housecultured

organismsorper

analyticalbatch

whenorganisms

arecommercially

supplied.

Lastplotteddatapoint

(LC50orEC50/25)must

bewithin2SDofthe

cumulativemean

(n=20)

Sample

Duplicate

5%totalproject

samplecount

(3peryear)

Recommended

acceptableRPD<20%

FieldBlank Oneperyear



controlwaterandthe

fieldblankwithinan

analyticalbatch

BottleBlank Twoperyear



controlaterandthe

bottleblankwithinan

analyticalbatch

4.4.3. Project-specificcorrectiveactionlimits

IndividualresultsproducedbytheDeltaRMParenotintendedtotriggerenforcementactions,

eventhoughcollectivelythedatamayguidemanagementactionsbyotherpartiesthrough

planning.Consequently,therearenoproject-specificcorrectiveactionsrequiredforthedata.

However,anycorrectiveactionsthatarewarrantedshallbemadeatthediscretionoftheQAO

followingtheSWAMPGuidance:

LaboratoryControlWater:Iftestedwithin-housecultures,affectedsamplesandassociated

qualitycontrolmustberetestedwithin24hoursoftestfailure.Ifcommercialculturesare

used,theymustbeorderedwithin16hoftestfailurefortheearliestpossiblereceipt.Retests

mustbeinitiatedwithin30hofreceipt,dependingontheneedfororganismacclimation.The

laboratoryshouldtrytodeterminethesourceofthecontrolfailure,documenttheinvestigation

anddocumentthestepstakentopreventarecurrence.

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ConductivityControlWater:Affectedambientsamplesandassociatedqualitycontrolmustbe

flaggedtoreflectthatconductivitymaybeoutsideofthephysiologicaltoleranceforthespecies

tested.

AdditionalControlWater:Basedontheobjectivesofthestudy,awatersamplethathassimilar

qualitiestothetestsamplemaybeusedasanadditionalcontrol(e.g.pHadjustments,

continuousaeration).Resultsthatshowstatisticaldifferencesfromthelaboratorycontrol

shouldbeflagged.Thelaboratoryshouldtrytodeterminethesourceofvariation,document

theinvestigationanddocumentthestepstakentopreventarecurrence.Thisisnotapplicable

forTIEmethodblanks.

ReferenceToxicantTests:IftheLC50exceeds+/-twostandarddeviationsoftherunningmean

ofthelast20referencetoxicantteststheassociatedambienttestshouldbeflaggedaseither

moreorlesssensitivethannormal.

FieldDuplicate:VisuallyinspectthesamplestodetermineifahighRPDbetweenresultscould

beattributedtosampleheterogeneity.Forduplicateresultsvaryingduetomatrix

heterogeneity,orwhereambientconcentrationsarebelowthereportinglimit,qualifythe

resultsanddocumenttheheterogeneity.ResultsthatdonotmeetSWAMPRPDcriteriashould

beflagged,regardlessofwhetherthesampleisheterogeneousornot.Theprojectcoordinator

shouldbenotifiedsothatthesamplingteamcanidentifythesourceofvariationandperform

correctiveactionpriortothenextsamplingevent.

FieldBlanks:Ifcontaminationofthefieldblanksandassociatedsamplesisknownorsuspected,

thelaboratoryshouldflagtheaffecteddata.Theprojectcoordinatorshouldbenotifiedsothat

thesamplingteamcanidentifythecontaminationsource(s)andperformcorrectiveactionprior

tothenextsamplingevent.

BottleBlanks:Ifcontaminationoftheequipmentblanksandassociatesampleisknownor

suspected,thelaboratoryshouldflagtheaffecteddata.Thelaboratorymanagershouldbe

notifiedsothatthelaboratorytechnicianscanidentifythecontaminationsource(s)and

performcorrectiveactionspriortothenextsamplingevent.

CalibrationStandard:Recalibratetheinstrument.Affectedsamplesandassociatedquality

controlmustbereanalyzedfollowingsuccessfulinstrumentrecalibration.

LaboratoryBlank:Reanalyzetheblanktoconfirmtheresult.Investigatethesourceof

contamination.Ifthesourceofthecontaminationisisolatedtothesamplepreparation,the

entirebatchofsamples,alongwiththenewlaboratoryblanksandassociatedQCsamples,

shouldbepreparedand/orre-extractedoranalyzed.Ifthesourceofcontaminationisisolated

totheanalysisprocedures,reanalyzetheentirebatchofsamples.Ifreanalysisisnotpossible,

theassociatedsampleresultsmustbeflaggedtoindicatethepotentialpresenceof

contamination.

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4.5. Performance-basedmethodconceptforthedeterminationofLT2pathogens(CryptosporidiumandGiardia)

TheDeltaRMPpathogen(CryptosporidiumandGiardia)monitoringisdesignedastheambient

monitoringcomponentoftheRegionalBoard’sBasinPlanAmendmenttoestablishaDrinking

WaterPolicytoprotectsourcewater,andisbeingconductedconcurrentlywiththedrinking

wateragencies’requiredLongTerm2(LT2)EnhancedSurfaceWaterTreatmentRule

monitoring(asdescribedintheDeltaRMPPathogenStudyDesignSummary).ThePathogen

Studyisintendedtosatisfydataneedsandmonitoringforanyfollow-uprequiredifBasinPlan

triggervaluesareexceededduringLT2monitoring.ThedirectionfromtheCentralValley

DrinkingWaterPolicyWorkgroupisthatdatacollectedfortheRMPpathogenmonitoring

shouldbeconsistentwithdatacollectedduringLT2monitoring.

EPAMethod1623wasdevelopedtosupportthesupportpromulgationofEPA’sLT2.Its

purposeistosupporttheassessmentofprotozoan(CryptosporidiumandGiardia)pathogenoccurrenceinrawsurfacewatersusedassourcewatersfordrinkingwatertreatmentplants.

EPAMethod1623providesqualitycontrol(QC)acceptancecriteriaforCryptosporidiumandGiardia,butnotesthatsomesamplematricesmaypreventtheacceptancecriteriafrombeing

met.EPAnotesthatfieldsampleswithmatrixspikerecoveriesbelowtheQCacceptancecriteria

identifiedinMethod1623(13%-111%forCryptosporidiumand15%-118%forGiardia)arevalid,andwillbeacceptedfordeterminingLT2binconcentrations.TobeconsistentwiththeLT2

data,theRMPwillconsiderdataoutsidetheacceptancecriteriatobevalid,butwillflagsuch

results.

ThePathogenStudymayuseEPAMethod1623.1,whichisreportedtohavehigher

Cryptosporidiumrecoveries.TheQCacceptancecriteriaidentifiedinMethod1623.1formatrix

spikerecoveriesare32%-100%forCryptosporidiumand8%-100%forGiardia.

TobeapprovedforLT2protozoantestingusingMethod1623and1623.1,laboratoriesare

requiredtodemonstrateacceptableperformanceforCryptosporidiumandGiardia.EPAMethod1623and1623.1areperformance-basedmethodsapplicabletothedeterminationof

CryptosporidiumandGiardiainaqueousmatrices.Demonstrationofacceptableperformance

includesinitialandongoingprecisionandrecoverytests,whichareconductedusingspiked

reagentwaterandmatrixsamples.Eachlaboratorythatusesthismethodisrequiredtooperate

aformalqualityassurance(QA)programthataddressesanddocumentsdataquality,

instrumentandequipmentmaintenanceandperformance,reagentqualityandperformance,

analysttrainingandcertification,andrecordsstorageandretrieval.Theminimumanalytical

requirementsofthisprogramconsistofaninitialdemonstrationoflaboratorycapability(IDC)

throughperformanceofaninitialprecisionandrecovery(IPR)test,andongoingdemonstration

oflaboratorycapabilityandmethodperformancethroughthematrixspike(MS)test,the

methodblanktest,anongoingprecisionandrecovery(OPR)test,stainingcontrols,andanalyst

verificationtests.Laboratoryperformanceiscomparedtoestablishedperformancecriteriato

determineiftheresultsofanalysesmeettheperformancecharacteristicsofthemethod.A

principalanalystverifiesthequalityandaccuracyofallsampleresults.Laboratoryperformance

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iscomparedtoestablishedperformancecriteriatodetermineiftheresultsofanalysesmeet

theperformancecharacteristicsofthemethod.Table4.11summarizestheminimumQC

requirementsforMethod1623,andTable4.12summarizestheminimumQCrequirementsfor

Method1623.1.DetailsaredescribedinEPAMethod1623 and1623.1.

Table4.11. QCrequirementsandacceptancecriteriafordeterminationofCryptosporidiumand

Giardiainaqueousmatrices(EPAMethod1623)

QCsampleor

procedure Frequency Acceptablelimits

Cryptosporidium

IPR Eachequipment/supplychange MeanRecovery=24-100%/RSD<55%

MethodBlank 1per20orweek Nofalsepositives

OPR 1per20orweek Recovery=22-100%

MatrixSpikes 1per20 Recovery=13-111%/RPD<61%

Matrix

Spikes/Duplicates

Initialuseandeachprocedural

changeRecovery=13-111%/RPD<61%

Positivestaining

controlEverybatch Nofalsenegatives

Negativestaining

controlEverybatch Nofalsepositives

Verificationofanalyst

performanceMonthly <10%differenceincounts

Giardia


MethodBlank 1per20orweek Nofalsepositives


MatrixSpikes 1per20 Recovery=15-118%/RPD<30%

Matrix

Spikes/Duplicates


changeRecovery=15-118%/RPD<30%

Positivestaining


Negativestaining




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Table4.12. QCrequirementsandacceptancecriteriafordeterminationofCryptosporidiumand

Giardiainaqueousmatrices(EPAMethod1623.1)

QCsampleor

procedure Frequency Acceptablelimits

Cryptosporidium


MethodBlank EachIPRandOPRset Nofalsepositives


MatrixSpikes 1per20 Recovery=32-100%/RSD<46%

Matrix

Spikes/Duplicates


change,andmulti-labvalidationof

modification

Recovery=32-100%/RSD<46%

Positivestaining


Negativestaining




Giardia


MethodBlank EachIPRandOPRset Nofalsepositives


MatrixSpikes 1per20 Recovery=8-100%/RSD<97%

Matrix

Spikes/Duplicates


change,andmulti-labvalidationof

modification

Recovery=8-100%/RSD<97%

Positivestaining


Negativestaining




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5. Special Training Needs and Certification

5.1. SpecializedTrainingorCertifications

BecausetheDeltaRMPusesperformance-basedmethodsforlaboratoryevaluation,laboratory

certifications(e.g.byNELAP/ELAP11)fortheanalysesplannedarepreferredbutnotrequired.

ThelaboratoryprovidinganalyticalsupporttotheDeltaRMPmusthaveadesignatedon-site

QAOfficerfortheparticularanalyticalcomponent(s)performedatthatlaboratory.This

individualwillserveasthepointofcontactfortheSFEI-ASCQAstaffinidentifyingandresolving

issuesrelatedtodataquality.

Toensurethatthesamplesareanalyzedinaconsistentmannerthroughoutthedurationofthe

program,keylaboratorypersonnelwillparticipateinanorientationsessionconductedduring

aninitialsitevisitorviacommunicationswithSFEI-ASCstaff.Thepurposeoftheorientation

sessionistofamiliarizekeylaboratorypersonnelwiththisQAPPandtheDeltaRMPQA/QC

program.Participatinglaboratoriesmayberequiredtodemonstrateacceptableperformance

beforeanalysisofsamplescanproceed,describedinsubsequentsections.Laboratory

operationswillbeevaluatedonacontinualbasisthroughtechnicalsystemsaudits,andby

participationinlaboratoryinter-comparisonprograms.

Personnelinanylaboratoryperforminganalyseswillbewellversedingoodlaboratory

practices(GLPs),includingstandardsafetyprocedures.Itistheresponsibilityoftheanalytical

laboratorymanager,and/orsafetystafftoensurethatalllaboratorypersonnelareproperly

trained.Eachlaboratoryisresponsibleformaintainingacurrentsafetymanualincompliance

withtheOccupationalSafetyandHealthAdministration(OSHA)orequivalentstateorlocal

regulations.Thesafetymanualwillbereadilyavailabletolaboratorypersonnel.Proper

proceduresforsafestorage,handling,anddisposalofchemicalswillbefollowedatalltimes;

eachchemicalwillbetreatedasapotentialhealthhazardandGLPswillbeimplemented

accordingly.

Personnelcollectingsamplesmusthavebeentrainedonthefieldsamplingmethodsdescribed

intheQAPP.Forpesticidesmonitoring,theUSGSfieldsamplingcoordinatorwillberesponsible

fortrainingtheUSGSfieldstaff.Forpathogenmonitoring,MWQIwillberesponsiblefortraining

thefieldstaff.Thesign-insheetofthetrainingcanbethedocumentationofthetraining.

11EnvironmentalLaboratoryAccreditationProgram(ELAP).ELAPprovidesevaluationandaccreditationofenvironmentaltestinglaboratoriesto

ensurethequalityofanalyticaldatausedforregulatorypurposestomeettherequirementsoftheState'sdrinkingwater,wastewater,shellfish,

food,andhazardouswasteprograms

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5.2. TrainingCertificationandDocumentation

Contractorsperformingsamplingareresponsibleforprovidingtrainingtotheirstaffand

maintainingrecordsofalltrainings.Thoserecordscanbeobtainedifneededfromcontractors

throughtheirrespectiveQAorSafetyOfficers.

5.3. TrainingPersonnel

Eachcontractlaboratory’sQAOfficerandSafetyOfficershallprovideand/ordesignatestaffto

providetrainingtotheirrespectivepersonnel.Allpersonnelresponsibleforsamplingwillbe

trainedinfieldsamplecollectionandsafetypriortothefirstdaytheyarescheduletosample

fortheDeltaRMP.

6. Documents and Records

AllDeltaRMPdocumentswillbeprovidedtotheSteeringCommittee,whichincludesthe

RegionalBoard.

SFEI-ASCwillcollectrecordsforsamplecollection,fieldanalyses,laboratorychemicalanalyses,

toxicitytesting,andpathogenanalyses.Samplessenttoanalyticallaboratorieswillincludea

Chain-of-Custody(COC)form.Theanalyticallaboratorieswillmaintainrecordsofsample

receiptandstorage,analyses,andreportedresults.

SFEI-ASCwillmaintainhardcopyorscannedfilesoffieldnotesandmeasurements,aswellas

laboratorysubmitteddocumentationandresultsattheSFEI-ASCmainoffice.TheSFEI-ASCData

Managerwillberesponsibleforthestorageandorganizationofinformation.

Contractlaboratorieswillalsoberesponsibleformaintainingcopiesofprojectdocumentation

originatingfromtheirrespectivelaboratories,withbackuparchivalstorageoffsitewhere

possible.AllSOPsusedfortheDeltaRMPwillbestoredindefinitelyincasefuturereviewis

necessary.

QualityAssuranceDocumentation

AlllaboratorieswillhavethelatestrevisionoftheDeltaRMPQAPP.Inaddition,thefollowing

documentsandinformationwillbecurrentandavailabletoalllaboratorypersonnel

participatingintheprocessingofProjectsamples,aswellastoSFEI-ASCprogramofficials:

1. LaboratoryQAPlan:Clearlydefinedpoliciesandprotocolsspecifictoaparticular

laboratory,includingpersonnelresponsibilities,laboratoryacceptancecriteriaand

correctiveactionstobeappliedtotheaffectedanalyticalbatches,qualificationofdata,and

proceduresfordeterminingtheacceptabilityofresults.

2. LaboratoryStandardOperatingProcedures(SOPs):Containinginstructionsforperforming

routinelaboratoryprocedures,suchaslogging,labelling,andstorageofsamples,cleaning

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ofequipment,checkingofreagents,etc.,thatarenotnecessarilypartofanyanalytical

methodologyforspecificanalytesoranalytetypes.

3. LaboratoryAnalyticalMethods:Step-by-stepinstructionsdescribingexactlyhowamethod

isimplementedinthelaboratoryforaparticularanalyticalprocedure.Containsallanalytical

methodsutilizedintheparticularlaboratoryfortheDeltaRMP.

4. InstrumentPerformanceInformation:Informationoninstrumentbaselinenoise,calibration

standardresponse,analyticalprecisionandbiasdata,detectionlimits,etc.Thisinformation

shouldbereportedfortheperiodsduringwhichDeltaRMPsamplesareanalyzed.

5. ControlCharts:Controlchartsareusefulinevaluatinginternallaboratoryproceduresand

arehelpfulinidentifyingandcorrectingsystematicerrorsources.Contractlaboratoriesare

encouragedtodevelopandmaintaincontrolchartswhenevertheymayservein

determiningsourcesofanalyticalproblems.

Copiesoflaboratorymethods,SOPs,andQAplansareavailablebyrequestfromtheSFEI-ASC

QAOfficer.SomelaboratorymethodsandSOPsmaybeeditedtoexcludeproprietarydetails

abouttheanalyses.Qualityassurancedocumentsarereviewedtoassureconformanceto

programneedsbytheDeltaRMPProjectManagerandQAOortheirdesignees.

CopiesofallrecordswillbemaintainedatSFEI-ASCandatthelaboratoryforaminimumfive

yearsafterprojectcompletion,afterwhichtheymaybediscarded,exceptforthedatabaseat

SFEI-ASC,whichwillbemaintainedwithoutdiscarding.Alldatawillbebackedupandsecured

ataremotelocation(i.e.,separatefromtheSFEI-ASCoffice).Asneeded,datarecoverycanbe

initiatedbycontactingtheback-upfacilityforrestorationandthiswillbecoveredthroughSFEI-

ASCoverhead.

AllparticipantslistedinTable0.1willreceivethemostcurrentversionoftheDeltaRMPQAPP.

6.1. ReportPackageInformation

Analyticalresults,includingassociatedqualitycontrolsamples,willbeprovidedtoSFEI-ASCby

theanalyticallaboratories.Thelaboratoriesanalyzesamplesaccordingtotheholdtimeslisted

intheDeltaRMPQAPP,butthefinalreportmaybefinalizedforreviewupto90daysafter

samplesarereceivedfromthelaboratory.Exceedancesofthestandardturnaroundtimeshould

bediscussedwithandapprovedbytheDeltaRMPProjectManagerandQAO.

Laboratorypersonnelwillverify,screen,validate,andpreparealldata,includingQA/QCresults,

inaccordancewiththeDeltaRMP’sQAPPandwillprovide(uponrequest)detailedQA/QC

documentationthatcanbereferredtoforanexplanationofanyfactorsaffectingdataquality

orinterpretation.AnydetailedQA/QCdatanotsubmittedaspartofthereportingpackage(see

below)shouldbemaintainedinthelaboratory’sdatabaseforfuturereference.

Laboratorieswillprovideelectroniccopiesofthetabulatedanalyticaldata(includingassociated

QA/QCinformationoutlinedbelow)inaformatagreeduponwiththeDeltaRMPProject/Data

Managerordesignee.

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EachElectronicDataDeliverable(EDD)reportwillconsistofthefollowing:AnalyticalandQA

dataresultsinanappropriateCEDENformatandCRMcertificates(whenapplicable).

ResultsshouldbeflaggedbythelaboratoryforexceedancesofDeltaRMPMQOsfor

completeness,sensitivity,precision,andaccuracy,usingdataqualitycodesasdefinedby

CEDEN’slistofcodes,whichhavebeenadoptedbytheDeltaRMPforreportingdata.Thedata

qualitycodesshouldbeprovidedintheLabResulttableintheResQualCodeandQACodefields.

AlistofcommonlyusedresultqualifiercodesandQAcodesareprovidedinTables6.1and6.2,

respectively.AcompletedlistofcodesisavailableonCEDEN’sControlledVocabularywebpage.

Detailsonthemeasurementsandproceduresthatareexpectedtobeusedtodemonstratethe

qualityofreporteddatacanbefoundinSection4,QualityObjectives,Criteria,andControl

ProceduresforMeasurementData.

6.1.1. AnalyticalandQAdataresults

ToxicitydatathatisgeneratedusingfundsprovidedbySWAMPwillbesubmittedtotheOffice

ofInformationManagementandAnalysis(OIMA)bythedataproviderusingSWAMPdata

templates,SWAMPformatting,completenessandbusinessrulesandthroughtheSWAMP’s

DataChecker.Thisonlinetoolalertsuserstodatathatdonotconformtothebusinessrules

outlinedintheapplicableSWAMPDataManagementPlanorthevaluesestablishedin

SWAMP’sLookUpLists.Datamustbereviewedandverifiedforformat,completeness,and

qualitycontrolrequirements,includingresultqualificationsandappropriatesampleandbatch

comments,priortosubmissiontoOIMA.Thelaboratorymustbereachabletoanswerquestions

regardingthedatasubmittalifnecessary.Ifthedataisdeterminedtobeincompleteor

requiringsignificantcorrections,thedatamaybereturnedtothelaboratoryforcorrectionand

re-submission.OncethesedatahavebeenapprovedbySWAMP,theappropriateSWAMPData

ManagerwillprovidethedatawithintheCaliforniaEnvironmentalDataExchangeNetwork’s

(CEDEN)electronicdatadeliverable(EDD)templatestoSFEI/ASCforfurtherprocessing.SFEI-

ASCstaffisencouragedtocontacttheOIMAHelpDeskwithanydataquestionstheymayhave.

Resultsforfieldmeasurements,pesticides,copper,DOC/POCandpathogenswillbesubmitted

intheEDDtemplatesuppliedbySFEI-ASC.Tabulateddatawillincludethefollowinginformation

foreachsample(whenapplicable):

1. Sampleidentification:UniquesampleID,sitecode,sitename,collectiondate,collection

time,analysisdate,sampletype(fieldorQCtypes),andmatrix(water).

2. Analyticalmethods:Preparation,extraction,andquantitationmethods(codesshould

referenceSOPssubmittedwiththedatasubmissionpackage).Alsoincludepreparation,

extraction,andanalysisdates.

3. Analyticalresults:Analytename,fraction,result,unit,methoddetectionlimit(MDL),and

reportinglimit(RL)foralltargetparameters.Theappropriatedataqualifiersshouldbe

submittedwiththeresults.

Requiredadditionaldatainclude:

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• Controlresults(fortoxicitytests)

• Summaryandindividualreplicateresults,includingwaterqualityparameters(for

toxicitytests)

• Labreplicateresults(andfieldreplicates,whensentforanalysis)

• Qualityassuranceinformationforeachanalyticalchemistrybatch:

• CRMorLRMresults:absoluteconcentrationsmeasured,certifiedvalue,and%recovery

relativetocertifiedorexpectedvalue.

• Matrix(orblank)spikeresults:includeexpectedvalue(native+spike)foreachanalyte,

actualrecoveredconcentrations,andcalculated%recovery.

• Methodblanksampleresultsinunitsequivalenttofieldsampleresults(e.g.,ifthefield

samplesarereportedasng/g,methodblanksaregiveninthesameunits).

• Fieldandlabreplicateresultsandcalculated%RPDor%RSD.

6.1.2. ElectronicDataDeliverableTemplate

SFEI-ASCisaRegionalDataCenter(RDC)fortheStateofCaliforniaandusestemplates,

standardizedvocabularyandbusinessrulesdevelopedandmaintainedbyCEDENtomanage

dataforfieldcollection,chemistry,taxonomy,tissue,toxicity,andbioassessmentsampling.

SFEI-ASCwillprovidetrainingandguidancetocollectionagenciesandlaboratoriesonhowto

usetheCEDENtemplates.

Priortofieldcollection,SFEI-ASCwillprovidethefieldcollectionagencyacopyoftheCEDEN

LocationsandChemResults tablestobepopulatedwithinformationaboutthesample

collection.

Priortoanalyses,SFEI-ASCwillprovidethelaboratorywithacopyoftheappropriateCEDEN

template(populatedbythefieldcollectionagencywithinformationaboutthesample

collection)anddocumentationforpopulatingtheCEDENtemplate.Thedocumentationdetails

attributesofeachfieldincludingfieldname,datatype,whetherthefieldisrequiredornot,the

appropriatelookuplistforapprovedvocabularyandadescriptionofeachfield.TheCEDEN

templatesanddocumentationareavailableon-linefromCEDENat

http://www.ceden.org/ceden_datatemplates.shtml.Lookuplistvaluesareavailableonthe

CEDENControlledVocabularywebsite.

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Table6.1.CEDENcontrolledvocabularyforresultqualifiers.

ResultQualifierName ResultQualifierCode

Absent A

Colonial COL

ConfluentGrowth CG

Cw/C-ConfluentGrowthwithColiforms w/C

Cw/oC-ConfluentGrowthwithoutColiforms /oC

DetectedNotQuantifiable DNQ

EqualTo =

FieldEstimated JF

GreaterThan >

Greaterthanorequalto >=

LessThan <

Lessthanorequalto <=

NoReportableSum NRS

NoReportableTotal NRT

NoSurvivingIndividuals NSI

NotAnalyzed NA

NotDetected ND

NotRecorded NR

PercentRecovery PR

Present P

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Table6.2.CommonCEDENQAcodes.

QACode QADescr

BRK Noconcentrationsamplecontainerbroken

BRKA Samplecontainerbrokenbutanalyzed

BS InsufficientsampleavailabletofollowstandardQCprocedures

DO Coelution

DS BatchQualityAssurancedatafromanotherproject

H Aholdingtimeviolationhasoccurred

IL RPDexceedslaboratorycontrollimit

IP Analytedetectedinfieldorlabgeneratedblank

IU PercentRecoveryexceedslaboratorycontrollimit

J Estimatedvalue-EPAFlag

M Amatrixeffectispresent

NBC Valuenotblankcorrected

None None-NoQAQualifier

R Datarejected-EPAFlag

SC SurrogateCorrectedValue

OtherQACodes

BB Sample>4xspikeconcentration

BE Lowsurrogaterecovery;analyzedtwice

BLM CompoundunidentifiedorbelowtheRLduetooverdilution

BT Insufficientsampletoperformtheanalysis

BY Samplereceivedatimpropertemperature

BZ Samplepreservedimproperly

CS QCcriterianotmetduetoanalyteconcentrationnearRL

CT QCcriterianotmetduetohighlevelofanalyteconcentration

D EPAFlag-Analytesanalyzedatasecondarydilution

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QACode QADescr

DRM Spikeamountlessthan5XtheMDL

EU LCSisoutsideofacceptancelimits.MS/MSDareaccept.,nocorr.

EUM LCSisoutsideofcontrollimits

FO Estimatedmaximumpossibleconcentration(EMPC)

GN Surrogaterecoveryisoutsideofcontrollimits

GR Internalstandardrecoveryisoutsidemethodrecoverylimit

H24 Holdingtimewas>24hoursforBacteriatestsonly

H6 Holdingtimewas>6hrsbut<24hoursforBacteriatestsonly

HH Resultexceedslinearrange;concentrationmaybeunderstated

HR Post-digestionspike

HT Analyticalvaluecalculatedusingresultsfromassociatedtests

IF Sampleresultisgreaterthanreportedvalue

JA Analytepositivelyidentifiedbutquantitationisanestimate

LC LaboratoryContamination

N TentativelyIdentifiedCompound

NC AnalyteconcentrationnotcertifiableinCertifiedReferenceMaterial

NMDL NoMethodDetectionLimitreportedfromlaboratory

NRL NoReportingLimitreportedbythelaboratory

PG Calibrationverificationoutsidecontrollimits

PJ Resultfromre-extract/re-analtoconfirmoriginalMS/MSDresult

PJM Resultfromre-extract/re-analtoconfirmoriginalresult

QAX WhenthenativesamplefortheMS/MSDorDUPisnotincludedinthebatchreported

RE Elevatedreportinglimitsdue to limited sample volume

SCR Screeninglevelanalysis

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6.1.3. StandardOperatingProcedures(SOPs)

ThelaboratorysubmittedSOPsforpreparation,extraction,andanalyticalmethodsupon

approvingtheQAPP.TheSOPsarelistedinAppendixDinthisQAPP.TheQAOfficer/Project

Managerwillneedtoapproveanychangesinmethods.

6.2. DataReportingRequirements

Eachlaboratoryshallestablishasystemfordetectingandreducingtranscriptionandcalculation

errorspriortoreportingdata.

DatawillbereportedinCEDENtemplatesorprovidedinacomparableformatapprovedby

SFEI’sDataManager.Chemical-analyticaldataandpathogendatawillbereportedinCEDEN’s

WaterQuality(WQ)template.ToxicitydatawillbereportedtoSWAMPusingtheSWAMP

toxicitytemplate.TheminimumfieldsrequiredfordatareportedintheCEDENWQtemplate

fortheLabResultstabare:StationCode,SampleDate,ProjectCode,CollectionTime,

CollectionMethodCode,SampleTypeCode,Replicate,CollectionDepth,UnitCollectionDepth,

LabBatch,AnalysisDate,MatrixName,MethodName,AnalyteName,FractionName,UnitName,

LabReplicate,Result,ResQualCode,MDL,RL,QACode.Thesefieldsshouldincludetruevalues

(notnulls).Otherfieldssuchaspreparationcodeandextractionmethodshouldbefilledoutto

theextentpossible.TheminimumfieldsrequiredfordatareportedintheCEDENWQtemplate

fortheLabBatchtabare:LabBatchandLabAgencyCode.BatchesmustbereviewedforQC

completenessandanydeviationinQCresultsshouldbedocumentedintheaccompanyingcase

narrative.Therequiredfieldswillbeidentifiedinthetemplateingreenfont.TheEDDtemplate

providedtothelaboratorybySFEIwillhavethefieldsconcerningfieldcollectionofthesamples

alreadypopulated.

Documentationcontainingdefinitions,fieldlength,fieldrequirement,andassociatedlookup

lists(ifapplicable)foreachfieldisavailableontheCEDENwebsite

(http://www.ceden.org/ceden_datatemplates.shtml)..Fieldsrequiringcontrolledvocabulary

canbeidentifiedbyhoveringoverthefieldnameinthetemplateandreferringtothelookup

listthatisreferenced.LookuplistsareavailableontheCEDENwebsiteat

http://www.ceden.org/CEDEN_Checker/Checker/LookUpLists.php.

OnlydatathathavemetMQOsorthathavedeviationsexplainedappropriatelywillbeaccepted

fromthelaboratory.WhenQArequirementshavenotbeenmet,thesampleswillbe

reanalyzedwhenpossible.Onlytheresultsofthereanalysisshouldbesubmitted,providedthey

areacceptable.

Reportingturnaroundtimesforsubmissionofresultsfromsampleanalysesarespecifiedin

contractswiththeanalyticallaboratories.However,samplesshouldbeextractedandanalyzed

withintheholdingtimesspecifiedfortheanalyticalmethodsused(Table9-2).Turnaroundtime

requirementsspecifiedinsubcontractsaregenerally90daysorless.

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6.3. DataStorage/Database

DataaremanagedbySFEI-ASCDataServicesasestablishedinSection13.Uponcompletionof

QA/QCreviewanddatavalidation,dataarecompiledintotheSFEI-ASCRDCdatabaseand

distributedtotheprojectmanagers.

DatathatareapprovedforpublicreleasearemadeavailablethroughSFEI-ASC’sContaminant

DataDisplayandDownload(CD3)tool,usuallywithinoneyearofsamplecollection.Datawill

alsobemadeavailablethroughCEDEN’sAdvancedQuerytool.

7. Sampling Process Design

7.1. StudyAreaandPeriod

Samplecollectionpointsandajustificationforsiteselectionforthedifferentelementsare

describedinthespecificdesignsforeachoftheDeltaRMPmonitoringelements(AppendixB).

TheDeltaRMPmonitoringsitesarelocatedinandupstreamoftheDelta(Figures3-1,3-2,and

3-3).Themonitoringsitesforpesticidesurfacewatersamplingrepresentkeyinflowstothe

Delta(Figure3-1).Themonitoringsitesformercurysamplingrepresentdifferentsubareasof

theDelta(Figure3-2).Ambientpathogenmonitoringsites(Figure3-3)areco-locatedwith

existingsitesoftheMunicipalWaterQualityInvestigationsprogram.

Samplingtimingandfrequencyvariesforthedifferentelementsofthemonitoringprogram:

• Pesticidesitesarevisitedmonthly.Themonthlyvisitscapturetwowetevents(firstflush

rain,2ndsignificantstorminwinter)andthreedryevents(1

stirrigation,2

ndirrigation,

andsnowmelt/springrunoff).

• Mercurymonitoringincludesannualsportfishsamplingatsixsitesandquarterlywater

samplingat5ofthesesites.Bothsportfishandwatersamplingwillbeginin2016.

• Pathogenambientwatermonitoringoccursmonthlyat12sites.

Collecteddataareusedtoevaluatefuturedataneedsandadjustthesamplingandanalysisplan

asneededtooptimizedatacollectioninanadaptivemanner.Theprogramwillbecontinually

adjustedtooptimizedatacollection.ThemonitoringdesignisdescribedintheMonitoring

DesignSummarydocument.

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7.2. SamplingSites

Table7.1. Samplingsitesandschedule.

SiteName SiteCode

Target

Latitude

Target

Longitude

Sampling

frequency

Sampling

Day

Pesticides

MokelumneR@NewHope

Road 544SAC00238.23611 -121.41889 Monthly 3

rdTuesday

SacramentoR@Hood 510SACC3A 38.36691 -121.52037 Monthly 3rdTuesday

SanJoaquinR@BuckleyCove 544LSAC13 37.97667 -121.37889 Monthly 3rdTuesday

SanJoaquinR@

Vernalis/AirportWay 541SJC50137.67556 -121.26417 Monthly 3

rdTuesday

UlatisC@BrownRd 511ULCABR 38.30667 -121.79472 Monthly 3rdTuesday

Mercury

LibertyIsland 38.24210 -121.68490Fish:Annually

Water:QuarterlyNotspecified

LittlePotatoSlough 38.09627 -121.49602Fish:Annually


MiddleR@BordenHwy(Hwy4) 37.89083 -121.48833Fish:Annually


MokelumneRdsCosumnesR 38.25528 -121.44000 Fish:Annually Notspecified

SacramentoR@Freeport 38.45570 -121.50120Fish:Annually


SanJoaquinR@

Vernalis/AirportWay541SJC501 37.67556 -121.26417

Fish:Annually


Pathogens

BanksPumpingPlant KA000331 37.81480 -121.61573 Monthly 1stWednesday

CacheSloughnrRyderIsland B9D81281401 38.22500 -121.67481 Monthly 1stTuesday

CalaverasR@UoPFootbridge B9D75891188 37.98003 -121.33648 Monthly 1stTuesday

ColusaBasinAgDrain A0294500 38.80197 -121.72552 Monthly 1stMonday

JonesPumpingPlant B9C74781351 37.79690 -121.58550 Monthly 1stWednesday

MokelumneR@BensonFerry B9D81531264 38.25461 -121.43658 Monthly 1stTuesday

NatomasEastMainDrainage

Canal A0V8367128038.61110 -121.467300 Monthly 1

stMonday

OldR@BaconIsland B9D75811344 37.96910 -121.57290 Monthly 1stMonday

RockSlough@CCWDFish

Facility B9C7586138537.99550 -121.70180 Monthly 1

stMonday

SacramentoR@Hood B9D82211312 38.36691 -121.52037 Monthly 1stTuesday

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SiteName SiteCode

Target

Latitude

Target

Longitude

Sampling

frequency

Sampling

Day

SacramentoR@WestinBoat

Dock B9D8322131038.53003 -121.53091 Monthly 1

stTuesday

SanJoaquinR@

Vernalis/AirportWay B070200037.67556 -121.26417 Monthly 1

stTuesday

8. Sampling Methods

Thequalityofsamplescollectedinthefieldisaddressedthroughanumberofprocedures.

Properselectionofequipment,suppliesandtrainingforuseofthoseitemsensuresthat

collectionproceduresandmaterialsdonotorminimallyaffectsamples.Collectionandanalyses

ofappropriatequalitycontrolsamplesallowsmeasurementandassessmentofartifactsor

influencesofsamplingonsamplecharacteristics,todifferentiateuncertaintiesandvariability

introducedbythesamplingprocessfromthoseinherentinthemonitoredsystem.Thissection

willdescribequalityassuranceandqualitycontrolproceduresimplementedfortheDeltaRMP.

8.1. FieldEquipmentandSupplies

Samplingequipmentandsupplieswillvarydependingontheprojectelement.Sample

containersappropriatetothematricesbeingsampledandtheanalysestowhichtheywillbe

subjectedwillbechosen.Allcontainersshouldmeetorexceedtherequiredtracelimits

establishedbytheUSEPAinthedocumentEPA/540/R-93/051,SpecificationsandGuidancefor

Contaminant-FreeSampleContainers.Chemical-resistantpowder-freenitrileandpolyethylene

gloveswillbewornandclean-handsdirty-handsprotocolswillbefollowedtominimize

contaminationofexposedsamples.Fieldcleaningproceduresofsamplingequipmentwillbe

employedtominimizecross-contaminationbetweensamplesfortheparametersofinterest.

FieldpersonnelwillrefertothedetailedworkplanfortheappropriateDeltaRMPsampling

elementtoensurethatallequipmentandsuppliesarebroughtinthefield.However,ata

minimumthefollowingsuppliesarerequiredfortherespectiveprojectelements:

Fish

Boats(electro-fishingand/orforsettingnets)

Waterprooflabels

Bonesaw,gillnets(varioussizes),filetknives,fishpicks,shackles,pliers,

sharpeningstone

Rodandreels,bait,tacklebox,landingnet,livebaitcontainer

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Plasticicechests,inflatablebuoy,floats,anchorchains,anchors,patchkit

Heavy-dutyaluminumfoil(prepared),zipper-closurepolyethylenebags

Ottertrawls

Blocks

Measuringboards,tapemeasure,idkeys,Tefloncuttingboards

Rodandreels,landingnet

Water

Samplingcontainersandlabels

Collectiondevicesappropriateforsite

Powder-freenitrilegloves

Fieldmeters

Deionizedwatersquirtbottle

Fieldsheet(seeAppendixE)

Coolersandice

Chain-of-custodyform(seeAppendixF)

8.2. FieldSampleCollectionandQualityAssuranceProcedures

8.2.1. SurfaceWaterSampleCollection

Samplesforpesticidemonitoringarecollectedmonthlyasgrabsamples0.5metersbelow

surface.SpecifictargetedeventssamplingdescribedinTable8.1willreplaceroutinemonthly

samplecollectionasappropriate.Thetriggersandcriteriaforeventssamplingaresummarized

inTable8.1.

Watersamplesformercurymonitoringarecollectedquarterly.

TheDeltaRMPPathogenStudyDesignSummaryspecifiesmonthlyambientmonitoringsample

collectionfortwoyearsbeginninginApril2015tomatchtheLongTerm2EnhancedSurface

WaterTreatmentRule(LT2)-requiredwatersupplyintakesamplecollection.MWQIwillcollect

grabsamplesateachofthelocationsshowninFigure7.2duringthefirstweekofeachmonth

onthesite-specificday.Thespecifiedsamplecollectiondepthforthepathogensamplingis1

meter/3feet.MWQImaypostponeorcancelsamplecollectionduetosafetyorlogistical

concerns.

ReferencesandlinksforaccessingSOPsforsurfacewatersamplecollectionareprovidedin

AppendixD.

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Table8.1. Samplingeventtriggersforpesticideeventssampling.

Event SamplingTriggers Criteria Notes

Wet

1stseasonalflush

(WaterYear)

• Guidanceplotsprojectsignificantincrease(~25%)in

flowatfoursites:lower

SacramentoRiver,lower

AmericanRiver,SanJoaquin

RiveratVernalis,and

MokelumneRiver.

• Precededby>30daysdryweather(SacSW

criteria).

• Sampleeventstohitallsitesin1to2days.

• WhenfavorablestormconditionsandrunoffareforecastcoordinatedirectlywithAHPlab.

• AlertAHPL7daysinadvanceofupcomingstormfororganismpreparationand2daysinadvanceaboutlikelihoodofadequateprecipitation

Significant

winterstorm

• Guidanceplotsprojectsignificantincrease(~25%)at

foursites:lowerSacramento

River,lowerAmericanRiver,

SanJoaquinRiveratVernalis,

andMokelumneRiver.

• Minimum2weeks

since1stflushsample

event.

• Ifcollectmorethan1eventsampleinthesamemonth,donotsampleinfollowingmonth.

• WhenfavorablestormconditionsandrunoffareforecastcoordinatedirectlywithAHPlab.

• AlertAHPL7daysinadvanceofupcomingstormfororganismpreparationand2daysinadvanceaboutlikelihoodofadequateprecipitation

Dry

EarlySpring • Notriggers,cansampleina

particularmonth(March-

April).

• None • Meanttocapturesnowmeltbutrecognizesignificantimpactofupstreamdams.

• CoordinatesamplingschedulewithAHPlab7ormoredaysinadvance.

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Event SamplingTriggers Criteria Notes

1stirrigation

seasonsampling

(latespring/

earlysummer)

• Notriggers,cansampleina

particularmonth(May-June).

• None • Meanttocapturelatewinterandspringpesticideapplications(poststorms).

• Accountforplanting/pesticideapplicationtiming.


2ndirrigation

seasonsampling

(latesummer)

• Notriggers,cansamplea

particularmonth(August).

• None • Meanttocapturesummerpesticideapplications(rice,etc.).

• Accountforplanting/pesticideapplicationtiming.


Collectionofwatersamplesforanalysisofpesticidesandtoxicitytesting:

USGSpersonnelwillcollectwatersamplesforanalysisofpesticides,dissolvedcopper,and

toxicitytesting.Atsiteswherestreamflowisaffectedbytides,sampleswillbecollectedonthe

ebbtide.Duetothelargevolumesofwaterrequiredpersite,perevent(40litersfortoxicity

testingand2-4litersforpesticideanalyses),allsampleswillbecollectedasgrabsamples.

Waterwillbecollectedbysubmergingpre-cleaned4liter(toxicity),1liter(pesticides)

combustedamberglassbottles,andacidrinsed3literTeflonbottles(copper,DOCandPOC)0.5

metersbelowthewatersurface(Table8.2).Samplebottlesfordissolvedcopperwillberinsed

threetimeswithsitewaterpriortofilling,andcontainerswillbefilledcompletely,leavingno

headspace,tominimizevolatilization.

Theamountofwatertobefilteredinordertoobtainasufficientquantityofmaterialforthe

POCanalysisdependsonthesuspended-sedimentconcentrationand/ortheconcentrationof

humicandothersubstancesthatcausecoloredwater,suchasorganicandinorganiccolloids.

Approximatesuspended-materialsconcentrationvolumeofsampletobefiltered(mL):

Suspendedmaterials

concentration(mg/L)

Volumeofsample

tobefiltered(mL)

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1–30 250

>30–300 100

>300–1,000 30

>1,000 10

Number,typeandtimingoffieldcollectedQA/QCsampleswillbedeterminedbytheUSGS

OCRLandwillmeetorexceedSWAMPguidelines

(http://www.waterboards.ca.gov/water_issues/programs/swamp/docs/mqo/6_syn_water.pdf.

Measurementsofbasicfieldparameters(temperature,pH,specificconductance,dissolved

oxygenconcentration,dissolvedoxygensaturation,andturbidity)willbemadeatthetimeof

samplecollectionusingaYSI6920V2multiparametermetercalibratedwithappropriate

standards.

Collectionofwatersamplesforanalysisofmercuryandmethylmercury:

MPSLpersonnelwillcollectwatersamplesforanalysisofunfilteredandfilteredtotalmercury,

unfilteredandfilteredmethylmercury,andancillaryparameters.Duringannualeventswhen

fishsamplingoccurs,watersampleswillbecollectedafterfisharecollected.Sampleswillbe

collectedaccordingtoMPSLFieldSOPv1.1;ultratrace-leveltechniquesforequipmentcleaning

andsamplecollection(USGSNationalFieldManual,ChapterA5.6.4.B.LowLevelMercury);and

theclean-handsdirty-handscollectionmethodswhereappropriate.Itisimportanttofollowthe

clean-handsdirty-handscollectionmethodwhencollectingtotalandmethylmercurysamplesto

avoidsamplecontamination.Adepth-integratedsamplewillbecollectedfromaboatatthe

pointinthechannelwheredischargeisthegreatest(Table8.2).Samplecollectionwilloccurin

anareawheretheboatdoesnotinterferewiththesample,withthecollectorwearingclean

polyethylenegloves.

Number,type,andtimingoffieldcollectedQA/QCsampleswillbedeterminedbyMPSLandwill

meetorexceedSWAMPguidelines(Table4.3)

Samplecontainerswillconsistof4-Lclearglassbottles(Table8.2).Sampleswillbeprocessed

andfilteredinthelab(MPSL).

Collectingandprocessingwatersamplesforanalysisofmercuryatalow(subnanogramper

liter)levelrequiresuseofultratrace-leveltechniquesforequipmentcleaning,sample

collection,andsampleprocessing.

Section9.1.describesfieldsamplehandlingandshippingproceduresandTable9.1.provides

informationonstorageandholdtimerequiremtns

CollectionofwatersamplesforanalysisofLT2pathogens:

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SampleswillbecollectedfortheDeltaRMPPathogenStudyfollowingthegeneralfield

proceduresdescribedintheMunicipalWaterQualityInvestigations(MWQI)ProgramField

Manual.SpecificprotocolsforCryptosporidiumandGiardiasamplingfollowEPAMethod1623.

MWQIwillcollectonefieldduplicatesamplepereventonasequentiallyrotatingsiteschedule.

MWQIwillfillone10-Lcubitainerforeachsampleandshippedtothelaboratoryonicefor

analysisbyEPAMethod1623(Holdtime:96hours).

MWQIwilluseastainlesssteelbucketandastainlesssteelfunnelforgrabsampling.MWQIwill

rinsesamplingdevicestwicewithambientwaterpriortosampling.Samplingdeviceswillbe

decontaminatedbetweensitesbyrinsingwithde-ionizedwater.MWQISampleCollection

Teamswillfilloutfielddatasheetsimmediatelyaftersamplecollection.Allsamplecontainers

willbelabeledwiththedate,locationsampledoruniquesiteID,parametertobemeasured,

andsamplepreparation(unfiltered).

Table8.2. Samplecontainertypeandvolumeusedforeachparametergroupforcollectionof

watersamples.(Section9.1providesmoreinformationonfieldsamplehandlingandshipping

procedures.Table9.1providesinformationaboutstorageandholdtimerequirementsforeach

parametergroup.)

Program

Element ParameterGroup Bottletype*

Numberofbottles/event

Sample

Volume/Site

Mercury

Tracemetals

Conventional12 Clearglass 5 4L

Pathogens Pathogens

LDPE

cubitainer 15 950mL

Pesticides Watertoxicity Amberglass 40

4L/bottlex8

bottles

Pesticides Pesticides Amberglass 5 1L

Pesticides DOC/POC Amberglass 5 125mL

Pesticides Copper Teflon 5 3L

8.2.2. FishSampleCollection

Sportfishsamplesformercurymonitoringarecollectedannually.Theappropriatesample

collectionmethodmayvarybysiteandwillbedeterminedbytheMPSLfieldsamplecollection

team.

ReferencesandlinksforaccessingSOPsforfishsamplecollectionareprovidedinAppendixD.

12Conventionalparameters(DOC,TSS,VSS)willbeanalysedinsamplealiquots.

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Collectionoffishtissuesamplesforanalysisoftotalmercury:

FishwillbecollectedinaccordancewithMPSL-102a,Section7.4.Becausehabitatsmayvary

greatly,thereisnoonemethodofcollectionthatisappropriate.Fieldcrewswillevaluateeach

fishingsitetodeterminethecorrectmethodtobeemployed.Potentialsamplingmethods

include,butarenotlimitedto:electroshocking,seining,gillnetting,andhookandline.Field

crewwilldeterminetheappropriatecollectionmethodbasedonphysicalsiteparameterssuch

asdepth,width,flow,andaccessibility.Fieldcrewwillindicatethecollectionmethodondata

sheets(AppendixE).

Thetargetedfishspeciesislargemouthbass.Thegoalistocollect11individualsspanninga

rangeoftotallengthfrom200-500mmateachsite(Table8.3).Specimensofsimilarpredator

speciesmaybecollectedifthedesirednumberofindividualsoftheprimarytargetfishspecies

inthedesiredsizerangecannotbecollectedatasite.

FurtherdetailsonsamplecollectioncanbefoundinMPSL-102a,Section7.4(seeAppendixD

forreferenceandlink).

Table8.3. Targetspecies,numberofindividuals,andsizerangesforcollectionoffishtissue

samples.

Program

Element

Parameter

Group PrimaryTarget1

Numberof

Individuals

Individuals/

Site(Size)

Mercury Mercury LargemouthBass 66

11total:2X(200-249

mm),2X(250-304mm),

5X(305-407mm),

2X(>407mm)

1Collectsimilarpredatorspecies(e.g.,smallmouthbass,Sacramentopikeminnow)ifprimarytargetisnotavailable.

8.3. CorrectiveAction

FieldSampling

Ifgoalsstatedforthecollectionofsamplesorthemeasurementofwaterqualityparameters

arenotachieved,sampleswillberecollectedormeasurementsrepeated,wherepossible,after

necessaryre-calibrationsofequipmentorre-evaluationofthesamplingscenario.Allnecessary

stepsforcorrectiveactionwillbedocumentedonthefieldformandonenteredintothe

electronicversionoftheFieldSamplingReportthatismaintainedbySFEI-ASC.Theindividuals

responsibleforassuringthatthefieldstaffareproperlytrainedandimplementtheField

SamplingSOPsaretheFieldCollectionCoordinators(i.e.,MPSLProjectCoordinator,MWQI

SampleCollectionTeamLead,andUSGSSamplingCoordinator),SFEI-ASCProjectManager,and

theQAOfficer.

Fieldsamplingqualitygoalsincludethemeetingofdataqualityobjectivesfor:

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• Completenessofsamplecollection

• Representativeness

• Accuracyandprecision(asindicatedbyfieldduplicates)

• Avoidanceofcontamination(asindicatedbyfieldblanks,equipmentblanks,andtravel

blanks)

Ifanydataindicatethatqualityobjectivesarenotbeingmet,FieldCollectionCoordinatorswill

consultwiththeirPrincipalInvestigator(ifapplicable),LaboratoryManager,andtheSFEI-ASC

QAOtodetermineifthefailureismostlikelyduetofieldorlaboratoryprocedures/methods.If

itisdeterminedthatfieldmethodsarethelikelycause,thePIwillworkwiththefieldsampling

teamtoensurethatprotocolsarebeingfollowedcorrectlyandifanyadditionalprotocols

(specifictothisproject)needtobeimplemented.

LaboratoryChemicalAnalyses

Ifchemicalanalyticallaboratoryresults13failtomeettheQArequirementsoutlinedintheDelta

RMPQAPPanditisdeterminedthatlaboratoryproceduresarethelikelycause,thenthePI(if

applicable)andLaboratoryManagerwillensurethatproperproceduresasoutlinedintheQAPP

arebeingimplementedandtodevelopanyadditionalprocedurestobringQAsampleresultsin

linewithmeasurementqualityobjectives.Correctiveactionswillbedocumented,resolved,and

followed-uponfollowingtheprocessforcorrectiveactionsthatisoutlinedbytheSWAMP.The

processisbasedontheSWAMPCorrectiveActionForm,andisappliedtosampleresultsthat

failtomeetthetechnicalandnon-technicalrequirementsofSWAMPanditsassociated

projects.

ToxicityTesting

DataQualityIndicatorsandtestacceptabilitycriteriafortoxicitytestingarelistedinTables4.7

and4.8,respectively.MQOsandTIEtriggersaresummarizedinTable4.9and4.10.TheAHPL

QAOwillbealertedwhenthesethresholdsareexceeded.TheAHPLQAOmaytakethe

followingactions,ifapplicable:

Ammonia:Whenasample’sammonia-nitrogenmeasurementexceeds5mg/L,theambient

samplemayberetestedatdifferentpHlevelstodeterminewhateffectammonia-nitrogen

levelshaveontestorganisms.Commensuratecontrolswillbeincludedanddataanalysiswill

followtheguidelinesprovidedandlistedinSWAMPChronicFreshwaterToxicityTestingQuality

ControlTable.

Conductivity:Whenasample’sconductivitymeetsorexceedstheacceptabilitythresholdfor

eachspecies,ahighorlowconductivitycontrolwillbeincludedinthetesttodetermine

13Includingchlorophylla.

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whetherhighorlowconductivitymayhavearoleinreducedmortality,growthorreproduction.

Alternatecontrolswillbenotedinthetoxicitytransformerworkbookandstatistical

comparisonswillfollowEPAandSWAMPguidelines.

DissolvedOxygen:Whenasample’sdissolvedoxygenexceeds8.6mg/L(forS.capricornutum,

C.dubiaandP.promelas;8.9mg/LforH.azteca)followingthesamplewarmingperiod,the

samplewillbegentlyaeratedpriortosamplerenewal,inordertodegasharmfuldissolved

gases.Ifasample’sdissolvedoxygenlevelislessthan4.0mg/L,theambientsamplewillbe

constantlyaeratedtoensureadequateoxygenlevelsforthedurationofthetest,aswellas

includingaconcurrentaerationcontrol.Dataanalysiswillfollowtheguidelinesprovidedinthe

SWAMPChronicFreshwaterToxicityTestingQualityControlTable.

pH:Whenasample’spHisbelow6orexceeds9,thesamplewillbetestedatitsoriginalpHand

alsoadjustedto7.5.ApHmethodblankwillalsobetestedthatincludesanadjustmenttothe

ambientsample’soriginalpHandthenreturnedto7.0.Dataanalysiswillfollowtheguidelines

providedintheSWAMPChronicFreshwaterToxicityTestingQualityControlTable.

Temperature:Sampletemperaturesmustnotdeviatebymorethan3°Cofthetargettest

temperatureforthedurationofthetest.Ifsampletemperaturesexceedthisrange,stepswill

betakentominimizesampletemperaturedeviations,suchasadjustingenvironmentalchamber

temperaturestoatighterrangeormovingatestintoamoretemperature-regulatedtesting

area,anddatawillbeflaggedaccordingly.

Toxicity:Ifasampletestspeciesexhibits>50%mortalitywithin96-hours,theAHPLQAOor

LaboratoryManagerwillcontacttheSFEI-ASCProjectManagerwithin24hourstodiscuss

potentialafollow-upwithatoxicityidentificationevaluationinordertodeterminewhatclass

ofchemical(s)iscausingtoxicity.TheDeltaRMPTAC’sTIEsubcommitteewilldecidepotential

TIEs.

Ifasampletestspeciesexhibits100%mortalityin48hours,theAHPLQAOorLaboratory

ManagerwillcontacttheSFEI-ASCProjectManagerwithin24hours,andadilutionseriestest

willbesetupassoonasorganismsareavailable(potentialcourierlimitations).

TestsareconductedaccordingtoproceduresandconditionsasdescribedintheSOPsprovidein

AppendixD.Beyondthoseidentified,deviationsfromtheserecommendedconditionsare

reportedtotheUCDAHPLQAO.ThePIandSFEI-ASCQAOandProjectManagerwillbenotified

ofthesedeviations.

IntheeventofanSOP/QAPPdeviationorcorrectiveaction,adeviation/correctiveactionform

willbeprepared,completed,signedandtheSFEI-ASCQAOandProjectManagernotified.Best

professionaljudgmentwillbeusedininterpretationofresultsobtainedwhenprotocol

deviationshaveoccurred.Alldeviationsandassociatedinterpretationswillbereportedin

interimandfinallaboratoryreports.ProtocolamendmentswillbesubmittedtotheSFEI-ASC

QAOandProjectManager.Uponapproval,protocolamendmentswillbeemployed.

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FailuretomeetIPRorOPRqualitycontrolacceptancecriteriaindicatessystemicproblemsthe

laboratorymustaddresspriortoprocessinganysamples.

9. Sample Handling and Custody

9.1. FieldSampleHandlingandShippingProcedures

Pesticides

Samplecontainerswillbelabeledwiththelocation,date,andtimecollectedandpackedinice

chestswithsufficientweticetomaintainsampletransportcriteria.Fieldsheetsandchain-of-

custodyforms(COC)willbefilledoutatthetimeofcollectionandwillincludesiteID,site

description,collectiondate/time,containertype,samplepreservation,fieldwaterchemistry

measurements,sampler(s)nameandrequestedanalyses.Allformswillbeincludedwiththe

appropriatesamplesduringshipping.Samplesforpesticideanalysiswillbedeliveredtothe

USGSOCRLlaboratoryinSacramentoCalifornia.IfuponarrivalattheOCRLsamplesarefound

tobewarm(icemelted)orifsamplecontainersarebrokentheProjectManagerandPrincipal

Investigatorwillbeimmediatelynotified.Icechestsareexaminedupondeliverytoensurethat

sampleshavebeenproperlychilled(acceptabletemperaturerange=0-6°C).

Watersamplesforpesticideanalyseswillgenerallybeprocessedtoextractionuponarrivalat

theOCRL.Ifthisisnotpossible,thesampleswillberefrigeratedat0-6°Cinthedarkfora

periodnottoexceedtheOCRLholdingtimerequirementof48hoursbetweensample

collectionandextraction.Uponarrivalofsamples,appropriatelaboratoryprocessingforms

notinguniquelaboratoryID,sitename,collectiontimeanddate,receivingtechnician’sname,

requestedanalysis,anddateandtimeofreceiptwillbefilledout.SignedcopiesofCOCswillbe

maintainedwiththeappropriateOCRLfieldandlaboratoryforms.

SamplesfordissolvedcopperanalysisandDOC/POCanalysiswillbeprocessedattheUSGS

OCRL,within24hoursofcollection.Samplesfordissolvedcopperanalysiswillbefilteredusing

0.45-micrometer(μm)filtersandacidifiedtopHlessthan2with2mlof7.5Nnitricacid.

SamplesforDOCanalysiswillbefilteredusing0.7-umporesize,pre-combustedglass-fiber

filters,collectedin125-mLbakedamberglassbottles,andacidifiedusing4.5Nsulfuricacid.The

0.7-umporesizefilterholdingtheretainedsuspendedmaterialwillbeusedforthePOC

analysisandwillbewrappedinanaluminumfoilsquareofappropriatesize.

Samplesfordissolvedcopper,DOC,andPOCwillbeplacedinacooleronweticeandshipped

overnighttotheUSGSNWQLinLakewood,CO.

Receipttemperatureandsamplecondition(broken/compromisedcontainers,incorrect

preservatives,holdingtimeexceedance,etc.)willberecordedbyreceivinglaboratories.

ToxicityTesting

ToxicitytestsampleswillbedeliveredtotheUCDavisAHPLaboratoryinDavis,California,

within24hoursofsamplecollection.UponarrivalatAHPL,toxicitytestingsampleswillbe

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immediatelyremovedfromtheice-chestsandthelaboratorystaffreceivingthecoolerswill

completetheaccompanyingCOC.TheAHPLwillinitiatetestswithin36hoursofsample

collection,althoughunderrarecircumstances,thisholdingtimemaybeextendedto120hours

forprecipitation-basedevents,whencourierdeliveryschedulesonweekendsandholidayslimit

theavailabilityoftestorganisms.IntheseinstancestheAHPLwillcontacttheSFEI-ASCQAO

andProjectManager,andassociateddatawillbeflaggedininterimandfinalreports.

Table9.1providesinformationaboutstorageandholdtimerequirementsforeachparameter

group.

Table9.1. Storageandholdtimerequirementsforeachparametergroup.

Parametergroup Storage

Holdtime

(CollectiontoExtraction,

whereapplicable)

Holdtime

(Extractiontoanalysis,

whereapplicable)

Storage

(Extractiontoanalysis,

whereapplicable)

Chlorophylla 0-6°Cindark

Filtrationwithin24hours

ofcollection 28days -20°Cindark

Copper,dissolved 0-6°Cindark

Filterinthefieldassoonas

possibleaftercollection 180days 0-6°Cindark

DOC/POC 0-6°Cindark

Filtrationwithin24hours

ofcollection

DOC:30days/POC:

100days 0-6°Cindark

Mercury,total

(Water) 0-6°Cindark

Preservewith0.5%v:v

pretested5%BrClor12N

HClwithin48hoursof

collection 90days Roomtemperature

Mercury,total

(Tissue) 0-6°Cindark

Coolto<6°Cwithin24hrs

ofcollection 1year -20°C

Mercury,dissolved


Filterandpreservewith

0.5%v:vpretested12NHCl

within48hoursof

collection 90days Roomtemperature

Methylmercury,

total


Preservewith0.5%v:v

pretested12NHClwithin

48hoursv 6months 0-6°Cindark

Methylmercury,

dissolved


Filterassoonaspossible

aftercollection;preserve

with0.5%v:vpretested

12NHClwithin48hoursof

collection 6months 0-6°Cindark

Pesticides 0-6°Cindark

Extractwithin48hoursof

collection Nottoexceed30days -20°Cindark

Toxicity 0-6°Cindark

InitiateTest36hafter

samplecollection NA NA

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Pathogens 1˚-20˚C

Elutewithin96hofsample

collection

7daysfrom

completionofslide

preparation 1˚-20˚C

Mercury

Samplecontainerswillbelabeledwiththelocation,date,andtimecollectedandpackedinice

chestswithsufficientweticetomaintainsampletransportcriteria.Fieldsheetsandchain-of-

custodyforms(COC)willbefilledoutatthetimeofcollectionandwillincludesiteID,site

description,collectiondate/time,containertype,samplepreservation,fieldmeasurements,

sampler(s)name,andrequestedanalyses.Allformswillbeincludedwiththeappropriate

samplesduringshipping.SampleswillbedeliveredtoMPSLinMossLanding,CA,orWPCLin

RanchoCordova,CA,asappropriate.Ifuponarrivalateitherlaboratory,samplesarefoundto

bewarm(icemelted)orifsamplecontainersarebrokentheProjectManagerandPrincipal

Investigatorwillbeimmediatelynotified.Icechestsareexamineduponreceipttoensurethat

sampleshavebeenproperlychilled(acceptabletemperaturerange=0-6°C).

WatersampleswillbedeliveredtoeitherMPSLorWPCLwithinrequisiteholdingtimes,where

laboratorypersonnelwillfilterpreservewatersamplesfollowingTable9.1.

Uponarrivalofsamples,appropriatelaboratoryprocessingformsnotinguniquelaboratoryID,

sitename,collectiontimeanddate,receivingtechnician’sname,requestedanalysis,anddate

andtimeofreceiptwillbefilledout.Samplesfordissolvedmercuryanddissolved

methylmercuryanalysiswillbefilteredusing0.45-micrometer(μm)filtersandacidifiedto0.5%

withpre-testedBrClasappropriatewithin48hoursofcollection.

Samplesforchlorophyllaanalysiswillbecollectedandfilteredusingasyringesamplemethod

within24hoursofcollection.Sampleswillbefilteredbyforcingwaterwitha60-mLsyringe

throughafilterholdercontaininga25-mmglassmicrofiberfilter.The60-mLsyringeandanin-

linefilterholderarerinsedthreetimeswiththeambientwaterbeforefiltration.Thesyringeis

thenfilledwith60mLofambientwater.Thefilterholderisthenremovedanda25-mmglass

microfiberfilterisplacedinside.Thefilterholderisthenscrewedontothesyringeandthe

ambientwateristhenflushedthroughthefilter.Thefilterholderisremovedeverytimemore

waterneedstobedrawnintothesyringe.Theprocessisthenrepeateduntilthedesired

amountofchlorophyllaispresent(usually60to360mLdependingonthewaterclarity).When

filteringiscomplete,thefilterholderisopenedandthefilterisremovedwithtweezerswithout

touchingthechlorophylla.Thefilteristhenfoldedinhalf,thenagain,inhalfwiththechlorophyllainsidethefolds.Thefoldedfilteristhenwrappedinaluminumfoilandplacedin

anenvelopelabeledwiththesiteinformationandthevolumefiltered.Theenvelopeisthen

immediatelyplacedondryiceuntiltransferredtoWPCL

Receipttemperatureandsamplecondition(broken/compromisedcontainers,incorrect

preservatives,holdingtimeexceedance,etc.)willberecordedbyreceivinglaboratories.

FishwillbeprocessedaccordingtoMPSL102a,exceptwherenotedhere.Collectedfishwillbe

partiallydissectedinthefield.Atthedock,thefishisplacedonameasuringboardcovered

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withcleanaluminumfoilorplastic;forkandtotallengtharerecorded.Weightisrecordedifthe

fishislargeenoughforthescale.Thefishwillthenbeplacedonthecoveredcuttingboard,

wherethehead,tail,andgutsareremovedusingacleancleaver(scrubbedwithMicro™,rinsed

withtapanddeionizedwater).ThefishcrosssectionistaggedwithauniquenumberedID,

wrappedinaluminumfoil,andplacedinacleanlabeledbag.Whenpossible,parasitesandbody

anomaliesarenoted.Thecleaverandcuttingboardarere-cleanedwithMicro™,rinsedwithtap

anddeionizedwaterbetweenfishspecies,persiteifmultiplestationsaresampled.

FurtherdetailsonsamplecollectionandprocessingcanbefoundintheSAP(SeeAppendixD

forreferenceandlink).

Fishsampleswillbewrappedinaluminumfoilandfrozenondryicefortransportationtothe

laboratory,wheretheywillbestoredat-20°Cuntildissectionandhomogenization.Lab

homogenateswillbefrozenuntilanalysisisperformed.Frozentissuesampleshavea12-month

holdtimefromthedateofcollection.Ifahold-timeviolationhasoccurred,datawillbeflagged

appropriatelyinthefinalresults.HoldingtimesforeachanalytecanbefoundinTable9.1.

Pathogens

AcourierwilldeliversamplestoBiovir(primarylab).Eurofinswillpickuponefieldduplicate

sampleperevent(secondarylab).Samplesmustbekeptonice.Thelaboratoriesmustelutethe

sampleswithin96hours(4days)ofsamplecollection.

10. Analytical Methods

10.1. FieldAnalytical/MeasurementMethods

Thefieldcollectionteamswillrecordmeasurementsperformedinthefieldonfieldsheets

(electronicorpaper)thenenterthemintoaCEDENtemplateforsubsequententryintheDelta

RMPdatabasebySFEI-ASC.Samplescollectedinthefieldaretobeplacedincontainersand

storedunderconditionsappropriatefortheanalysestobeperformed.Anyunusualsample

characteristicsorcircumstancespreventingnormalsamplehandlingwillalsobenotedinthe

fieldsheet.Onreturnfromthefield,thesamplingcrewwillpreparesamplesforimmediate

shippingtoanalyticallaboratoriesorstorethemunderappropriateconditionsforsubsequent

shipping

Tominimizediscrepancyinfieldresultsandprovideuseful,accuratescientificdata,all

personnelparticipatinginfieldsamplingarerequiredtofollowtheguidelinessetoutinthe

USGSNationalFieldManualfortheCollectionofWater-QualityData(forpesticideelement),

theMPSLFieldSOPv1.1(formercuryelement),andtheMWQIProgramFieldManual(for

pathogenstudy).

Operationofanyfieldinstrumentsshouldbecheckedatleastonedaybeforesampling.If

failureofaninstrumentshouldoccur,abackupinstrumentshouldbecheckedandcalibrated.

Allsamplingandmeasurementmodificationsorfailuresthatoccurinthefielddueto

instrumentmalfunctionwillberecordedontheFieldFormandtheFieldReferenceSheet.The

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FieldCollectionCoordinators,SFEI-ASCProjectManager,andtheQAOwillberesponsiblefor

ensuringthatstaffdocumentsalldeviationsfromplannedoperationsandschedulerepairs

and/oradditionaltrainingasneeded.

10.2. LaboratoryMethods

Forthemethodsselectedforaparticularapplication,theLaboratoryProjectManagermustbe

abletodemonstrateanddocumentthatthemethodsperformancemeetsthedataquality

requirementsoftheproject.Twoseparatefactorsareinvolvedindemonstratingmethod

applicability:First,demonstratingthatthelaboratorycanperformthemethodproperlyina

cleanmatrixwiththeanalyticalsystemundercontrol,andsecond,demonstratingthatthe

methodselectedgenerates“effectivedata”inthematrixofconcern.Theformeraddresseslab

oroperatortrainingandproficiency,whilethelatterdemonstratesthattheselectedmethod

performswiththeappropriateselectivity,sensitivity,biasandprecision,intheactualanalytical

matrix,toachieveprojectgoals.

Table10.1providesasummaryofanalyticalmethodsandinstrumentsusedbytheDeltaRMP.

Table10.1. Summaryofanalyticalmethodsandinstruments.

Parametergroup Methods Instrument Proprietary?

Chlorophylla InVitrodeterminationbyvisible

spectrophotometry(EPA446.0)Genesis10S No

Copper,dissolved

Collision/reactioncellinductively

coupledplasma–massspectrometry

(USGSTM-5-B1)

cICP-MS

(Agilent7500ce)No

DOC

UVcatalyzedpersulfateoxidationand

infrared(IR)spectrometry(USGSTest

MethodO-1122-92)

CarbonAnalyzer,

DohrmannDC-80,

DC-180,or

equivalent,witha

directconcentration

read-out.

No

DOCOrganicCarbon,Total(Combustionor

Oxidation)(EPA415.1)

TeledyneTekmar

TOCTorchNo

Mercury(Tissue)

Thermaldecompositionamalgamation

andatomicabsorption

spectrophotometry(EPA7473)

MilestoneDMA80 No

Mercury(Water)

Oxidation,purgeandtrap,andcold

vaporatomicfluorescence

spectrometry(EPA1631,RevisionE)

Tekran2600 No

Methylmercury

(Water)

Distillation,aqueousethylation,purge

andtrap,andcoldvaporatomic

fluorescencespectrometry(EPA1630)

Tekran2700 No

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Parametergroup Methods Instrument Proprietary?

POC Elementalanalysis(EPA440.0)

CarbonAnalyzer,

DohrmannDC-80,

DC-180,or

equivalent,witha

directconcentration

read-out.

No

Pesticides14

GasChromatography/Mass

Spectrometry

(USGSTM-5-B1)

Agilent7890GCwith

a5975cmass

spectrometerwitha

DB-5mscolumn(30

m×0.25mm×

0.25µm,Agilent)

No

PesticidesLiquidchromatographywithtandem

massspectrometry(LC/MS/MS).

Agilent1260HPLC

coupledtoa6430

tandemMSsystem

withaZorbaxEclipse

XDB-C18column(2.1

mm×150mm×3.5

mm;Agilent).

No

10.2.1. LaboratorySOPs

AllanalyticalmethodSOPscanbedownloadedfromtheSFEI-ASCGoogleDrive,unlesstheSOPs

areproprietary.CopiesoflaboratorySOPsarealsostoredatSFEI-ASCbutcannotbereleasedto

anyexternalpartieswithoutpriorconsentofthelaboratorywhentheyaremarkedas

proprietary.

10.2.2. CorrectiveActionsProcedures

CorrectiveactionsproceduresforanalyticallaboratoriesaresummarizedinTable10.2.

AdditionaldetailsaredescribedinSection4.3.

Table10.2. Correctiveactionsproceduresforanalyticallaboratories.

14 SeeTable3.2foradetailedlistoftargetanalytesforeachmethod

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LaboratoryQCSampleType Correctiveaction

MatrixSpikes

Thespikinglevelshouldbenearthemidrangeofthe

calibrationcurveoratalevelthatdoesnotrequiresample

dilution.Reanalyzethematrixspiketoconfirmtheresult.

Reviewtherecoveryobtainedforthematrixspike

duplicate.ReviewtheresultsoftheotherQCsamples(such

asreferencematerials)todetermineifotheranalytical

problemsareapotentialsourceofthepoorspikerecovery.

FieldBlanks

Iftargetanalytesarefoundinfieldblanks,samplingand

handlingprocedureswillbereevaluatedandcorrective

actionstaken.Thesemayconsistof,butarenotlimitedto,

a)obtainingsamplingcontainersfromnewsources,b)

trainingofpersonnel,c)discussionswiththelaboratory,d)

invalidationofresults,e)greaterattentiontodetailduring

thenextsamplingevent,orf)otherproceduresdeemed

appropriate.

FieldReplicate

Ifcriteriaareexceeded,fieldsamplingandhandling

procedureswillbeevaluatedandproblemscorrected

throughgreaterattentiontodetail,additionaltraining,

revisedsamplingtechniques,orotherproceduresdeemed

appropriatetocorrecttheproblems.

10.3. SampleArchiveandDisposal

ProjectsampleswillnotbedisposedofuntilallanalysesarecompleteandanalyticalandQC

resultshavebeenreviewedandapprovedbytheProjectManagerandtheQAO.

11. Instrument/Equipment/Supplies

ContractlaboratoriesmaintainequipmentinaccordancewiththeirrespectiveSOPs,which

includethosespecifiedbythemanufacturerandthosespecifiedbythemethod.Underthe

performance-basedapproach,theadequacyofcontractlaboratorytesting,inspection,and

maintenanceproceduresaredeterminedthroughregularreviewofresultsforanalysisoffield

andQCsamplesforallsubmitteddata.

Priortouseinthefield(typicallywithin24hourspriortosampling),handheldwaterquality

instrumentsarecalibratedagainstappropriatestandardsand,ifpossible,checkedagainsta

standardfromadifferentsource.Forsomemeasurementssuchasdissolvedoxygen,probesare

oftencalibratedtoambientconditions(water-saturatedair)ratherthantoknownstandards.In

suchcases,thefieldstaffshouldverifyappropriatequalitativeinstrumentresponse(e.g.in

waterdeoxygenatedbysparging,sodiumsulfiteaddition,orothermeans).Allcalibrationsare

documentedonacalibrationchecklistontheindividualinstrumentoritscasewithdate,time,

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andoperatorname.Ifaninstrumentcannotbecalibratedorisnotreadingcorrectly,abackup

instrumentwillbeusedtomeasurewaterqualityparameters.

Forsingleormultiparameterwaterqualitymeters,thefollowingstandardsaretypicallyusedto

calibrate:

1. pH–commerciallyavailablestandardspH4,7,10.Performa2-pointcalibrationcovering

therangeofexpectedmeasurements.Usethe3rdpHstandard(orstandardsuppliedby

anothermanufacturer)asacheckstandardtoverifycalibrationaccuracy.

2. SpecificConductance–performasingle-pointcalibrationandusetwocheckstandards

bracketingtheexpectedmeasurementrange.

3. Dissolvedoxygen–usecalibrationprocedurerecommendedbymanufacturer,typicallyin

water-saturatedair.

4. Temperature–checkagainstthermometerofknownaccuracyatleastyearly(preferably

quarterly).Anicewaterbathofapproximately0°Ccanbeusedtosemi-quantitativelyverify

temperatureproberesponsebutmayvaryduetouncontrolledfactorssuchascontainer

sizeandgeometry,ice/waterdisequilibrium,orthepresenceofmeltingpoint-lowering

contaminants.

LaboratoriesmaintaincalibrationpracticesaspartoftheirmethodSOPs.Calibration

proceduresaredescribedgenerallybelow.

Uponinitiationofananalyticalrun,aftereachmajorequipmentdisruption,andwheneveron-

goingcalibrationchecksdonotmeetrecommendedMQOs,thesystemwillbecalibratedwitha

fullrangeofanalyticalstandards.Immediatelyafterthisprocedure,theinitialcalibrationmust

beverifiedthroughtheanalysisofastandardobtainedfromadifferentsourcethanthe

standardsusedtocalibratetheinstrumentation,preparedinanindependentmanner,and

ideallyhavingcertifiedconcentrationsoftargetanalytes(e.g.,acertifiedsolution).The

calibrationcurveisacceptableifithasanr2of0.995orgreaterforallanalytespresentinthe

calibrationmixtures.Ifnot,thecalibrationstandards,aswellasallthesamplesinthebatch,

mustbere-analyzed.Allcalibrationstandardswillbetraceabletoanorganizationthatis

recognizedforthepreparationandcertificationofQA/QCmaterials(e.g.,NIST,NRCC,USEPA,

etc.).

Calibrationcurveswillbeestablishedforeachanalyteandbatchanalysisfromacalibration

blankandamulti-pointcalibration(asdescribedorrequiredinthemethod),coveringtherange

ofexpectedsampleconcentrations.Iftheinstrumentresponseisdemonstratedtobelinear

overtheentireconcentrationrangetobemeasuredinthesamplesanduseofasingle-point

calibrationisdescribedorallowedinthemethod,theuseofacalibrationblankandonesingle

standardthatishigherinconcentrationthanthesamplesmaybeappropriate.Otherwise,only

datawithintheworkingcalibrationrange(abovetheMDL)shouldbereported(i.e.

extrapolationisnotacceptable).Samplesoutsidethecalibrationrangewillbedilutedas

appropriate,andreanalyzed.

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LaboratoriesmaintaininternalSOPsforinspectionandqualitycheckingofsupplies.Undera

PBMSapproach,theseproceduresarepresumedtobeeffectiveunlessfieldandQCdatafrom

analysesindicateotherwise.SFEI-ASCwillthenworkwiththelaboratorytoidentifythecauses

andaddressdeficienciesintheSOPsthatresultedinthoseproblems.Iftheproblemisserious

andcannotbecorrectedbythelaboratory,theSFEI-ASCProjectManagerandQAOwilldiscuss

andidentifyalternatives,includingchangingthesamplingmaterialsandmethods,the

extractionandanalyticalmethods,thelaboratory,oranycombinationofthese.

12. Non-direct Measurements (Existing Data)

Non-DeltaRMPdata(e.g.,IrrigatedLandRegulatoryProgram)maybeusedindetermining

rangesofexpectedconcentrationsinfieldsamples,characterizingaverageconditions(e.g.,

temperature,barometricpressure)forcalculations,andotherpurposes.Thesedatawillbe

reviewedagainstthedataqualityobjectivesstatedinSection4andusedonlyiftheymeetallof

thespecifiedcriteria.DatanotmeetingMQOsshouldbeusedonlyinaqualitativemannerfor

developingconceptualmodelsandprioritizingfuturedataneeds.

Hydrologicdata(stage,flow,etc.)willbeobtainedfromexistinggaugesandrecorderslocated

atorneardesignatedmonitoringlocations

TheDeltaRMPwillnotconductanyadditionalmonitoringofpesticidechemistryandtoxicityin

sediments.Instead,sedimenttoxicityandchemistrydatacollectedbytheSurfaceWater

AmbientMonitoringProgram(SWAMP)StreamPollutionTrends(SpoT)monitoringwillbe

includedintheinitialassessment.TheSpoTQAPPisavailableontheSPoTwebsite.

13. Data Management

ThecollectionagenciesandlaboratoriesprovidedatatoSFEI-ASCinappropriateCEDEN

templates(asprovidedbySFEI-ASC)withinthetimeframestipulatedinthecontract,usually90

daysorless.Thelaboratoriesshouldusethecurrenton-linedatacheckertoreviewdatafor

vocabularyandbusinessruleviolationspriortosubmittingtoSFEI-ASC([email protected]

thecurrentURL).SFEI-ASCwillworkwiththelabstoaddressvocabularyandbusinessrule

issuesidentifiedfromusingthedatachecker.SFEI-ASCwillworkwithCEDENtopopulatethe

lookuplistswithnewvaluesasidentifiedbythelabsfromusingtheon-linedatachecker.

ToxicitydatathatisfundedbySWAMPshouldbesubmittedtoSWAMPbythedataprovider

usingSWAMPtemplatesandtheSWAMPdatachecker.Oncethesedatahavebeenapproved

bySWAMP,theSWAMPDataManagershouldprovidethedatainCEDENEDDtemplatesto

SFEI/ASCforfurtherprocessing.

ThelaboratoriesshouldreportdataasoutlinedinSection6.2,DataReportingRequirements.

DataaremaintainedatSFEIasestablishedinSection6.SFEI-ASCtrackseachdataset,from

submittaltofinaluploadtotheRDCdatabase.Onceallexpecteddatahavebeenreceived,

expertstaffonSFEI-ASC’sDataServicesteamprocessthedatausingaseriesofqueries

designedtoidentifyanyissuesremainingwiththeformatofthedata.TheQAOfficeror

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designeethenreviewsdataforqualityassuranceandqualitycontrolandappropriateCEDEN

QAcodesareappliedtothedataset.TheQAofficerordesigneewritesareportforeachdataset

outliningthequalityofthedata.Thisreporthighlightsanyissuesthatneedtobeaddressedby

thelaboratory,projectmanager,ordatamanagementstaff.

TheQASummaryReportincludesthefollowingdetails:

Lab

Matrix

Analyte

ReportingIssuesforLabtoReview

FormattingIssuesforDataManagertoReview

QAReview

Datasetcompleteness

Overallacceptability

MDLssensitivity

QBaverages(procedural,fieldblank)

Averageprecisionfromreplicatefieldsample

Accuracy(usingavarietyofSRMsorMatrixspikeQRECs)

Comparisonofdissolvedandtotalphases

Comparisontopreviousyears

RatioCheckingSummary

SumsSummary

Inaddition,specializedseniorscientistsfurtherrevieworganicsdatasetssuchasPCBs,PBDEs,

andpesticides.DataarethencompiledintotheRDCdatabaseanddistributedtotheproject

managers.DatathatareapprovedforpublicreleasearemadeavailablethroughSFEI-ASC’s

ContaminantDataDisplayandDownloadtool(CD3),usuallywithinoneyearofsample

collection.DatawillalsobemadeavailablethroughCEDEN’sAdvancedQuerytool.Thecontact

individualresponsibleforstepsandtasksofdatamanagementisAmyFranz.

SFEI-ASCmaintainsregularbackupsoftheirenterprisedatabasesbothtodiskandtape,nightly

andweekly,respectively.TheRDCdatabase,specifically,isalsobackeduphourly.Asafurther

protectivemeasure,copiesofthetapesetsarestoredbothonsiteandoffsite.Thelifetimeof

thebackupfilesontapeisabout2-3weeks.Additionally,abackupoftheRDCdatabasefrom

thefirstofeverymonthisstoredondiskindefinitely,allowingforquickrestoreandreviewof

archiveddataastheneedwarrants.

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14. Assessment and Response Actions

Initially,adesktoporon-siteperformanceauditwillbeperformedbytheQAOanddesignated

stafftodetermineifeachlaboratorycanmeettherequirementsoftheQAPPandtoassistthe

laboratorywhereneeded.ReviewofcurrentNELAPand/orstateELAPcertificationofa

laboratoryfortheanalysesperformedfortheDeltaRMPmaybeacceptedinsomecasesinlieu

ofanon-siteaudit.Reviewsmaybeconductedatanytimeduringthescopeofthestudy.

Resultswillbereviewedwithparticipatinglaboratorystaffandcorrectiveactionrecommended

andimplemented,wherenecessary.Furthermore,laboratoryperformancewillbeassessedon

acontinualbasisthroughlaboratoryintercomparisonstudies(roundrobins)whereavailable,

suchasthoseconductedbytheNationalInstituteofStandardsandTechnology(NIST).

Ifdataqualityissuesareidentified,apreliminarymeetingwillbeheldbetweenSFEI-ASC’sQAO

andtheProjectManagertodiscusspossiblesolutions.Ifnecessary,acorrectiveactionplanwill

bedevelopedinconsultationwiththeappropriatelab(s),thecorrectiveactionstaken,andthe

issueanditsresolutionsummarizedinabriefreportormemorandum.Asummaryofthese

issueswillbemaintainedintheProjectfiles,andwillbenotedinanyreportingthatincludes

affecteddata.

15. Reports to Management

ThereportinggoaloftheDeltaRMPistogeneratecommunicationproductsthatinformand

educatetargetaudiencesaboutDeltawaterqualityconditionsandtrends.Theinformationin

suchproductsistargetedatthehighestpriorityquestionsfacedbymanagers.Theprogram

achievesitsfullvalueonlytotheextentthatthedataitproducesaresynthesized,interpreted,

andreportedinamanneraccessibletoitsvariousaudiences.TheDeltaRMPwillproduce

anAnnualMonitoringReport,whichdocumentstheactivitiesoftheprogrameachyear;an

interpretivemainreport(ThePulseofTheDelta)thatsummarizesmonitoringresultsand

synthesizestheinformationtheyprovide;andtechnicalreportsthatdocumentspecificstudies

andsynthesizeinformationfromdiversesourcesinrelationtospecifictopicsandprioritized

assessmentquestions.

TheAnnualMonitoringReportwillpresenttheresultsofthepreviousJuly-Junefiscalyearof

sampling.Interpretationoftheresultswillbeverybasic.Themainpurposeofthisreportisto

sharethefinaldatawithprojectpartnersandcollaboratorsinatimelyway.TheAnnual

MonitoringReportalsoincludesaQAmemothatsummarizesanyQAproblemsanddocuments

anynon-conformanceswiththeQAPP.

TheQAOisresponsibleforsummarizingpotentialQAissueswithreporteddataand

communicatingthoseissuestotheProjectManager.TheQAOalsoreviewsanySFEI-ASC

analysesandreportsgeneratedfromthedata,toensurethatQAissuesareappropriately

acknowledgedandaddressed.

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16. Data Review, Verification, and Validation

AfterdataaresubmittedandincludedintheDeltaRMPdatabase,SFEI-ASCstaffexaminesthe

datasetforcompleteness(e.g.,correctnumbersofsamplesandanalyses,appropriateQC

sampledataincluded)andaccuracy(e.g.,insampleIDs),andspot-checkforconsistencywith

hardcopyresultsreportedbythelaboratory.TheSFEI-ASCQAOordesigneewillexamine

submittedQAdataforconformancewithMQOs,specifiedpreviously(Section4).Datathatare

incomplete,inaccurate,orfailingMQOswithoutappropriateexplanationwillbereferredback

tothelaboratoryforcorrectionorclarification.TheProjectManagerandQAOwilldiscussdata

failingMQOswithlaboratorystafftodeterminewhethermodificationstoanalyticalmethods

canbemadetoimproveresultsonreanalysis.Ifproblemscannotbereadilycorrected

(insufficientsample,irremovableinterferences,orblankcontaminationbasedonpastattempts

withthelab),resultsoutsidetheMQOswillbeflaggedusingCEDENcodesappropriateforthe

specificdeviationstoalertdatauserstouncertaintiesinquantitation.Resultsgreatlyoutside

thetargetMQOrange(z-scoresorp-scores>2,e.g.,foracceptancecriteriaof±25%,>±50%)15

maybecensoredandnotreported.

Inadditiontocontaminationandotherartifactsintroducedbysamplingandanalytical

methods,errorsmayariseatmanypointsintheprocessingandtransmittalofdatagenerated

fortheDeltaRMP.Characteristicsofreporteddataareexaminedtoidentifypossibleproblems

inthegenerationandtransmissionofdata.DatasubmittedtotheDeltaRMParecomparedto

valuesintheliteratureforcomparableenvironmentsandfrompreviousmonitoringtoevaluate

iftheyarewithintheexpectedrangeofvaluesforagivenstudy.Simplestatistics(e.g.,

minimum,maximum,mean,median)maybegeneratedtoquicklyidentifydatasetsor

individualdatapointsgreatlyoutsideoftheirexpectedrange.Anomalousindividualpointswill

beexaminedfortranscriptionerrors.Unitconversionsandsamplequantitationcalculations

maybereviewedtoidentifylargerandsystematicerrors.

17. Verification and Validation Methods

DataaresubmittedtoSFEI-ASCinelectronicform.TheQAOordesignatedprojectstaffverify

thatresultsforappropriatefieldandQCsamplesarereportedbycomparingthesampletypes

andnumbersprovidedagainstthosespecifiedinthedetailedprojectplan,chainofcustody

forms,and/orcontracts.Revieweddataarerecordedascheckedbyinitialsanddatestoensure

thatelectronicandhardcopyreportsagree.Thecontractlaboratory’sQAOfficer(QAO)

performschecksofallofitsrecordsandthelaboratory’sDirectororProjectManagerwill

recheck10%.AllchecksbythelaboratorymaybereviewedbySFEI-ASC.Issuesarenotedina

narrativelistandcommunicatedtothefieldorlaboratoryteamsasneededtocorrectany

problemsfound(e.g.unanalyzedsamplesleftinstorage,transcriptionerrors).

15z-score=|result–expectedvalue|/acceptabledeviation.SeeSection4.3.1.LaboratoryQCMeasurementsforadefinitionofthep-score.

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Aspartofthevalidationprocess,dataareevaluatedasmeetingorfailingMQOs.

ExceedancesofMQOsnotalreadynotedbythelaboratoryareflaggedinanyelectronic

databasesandcommunicatedtotheanalyzinglaboratoryforpossiblerecalculationand/or

reanalysis.Reconciliationandcorrectionoferrorsinreporteddatawillbeaddressedby

consultationamongSFEI-ASC’sProjectManager,SFEI-ASC’sQAO,andSFEI-ASCAnalyst(s)with

theLaboratory’sQAO,Laboratoryand/orProjectManager,andappropriatelabpersonnel.The

involvedpartieswillagreeuponanycorrections.

AnalysessometimesproduceresultsthatfailMQOsandmaynotbepossibletoovercomefora

smallnumberofanalyteswithinalargegroupofrelatedcompounds.Forexample,theremay

becontaminationthatisimpossibletoeliminateforallanalytes,whenanalysesareconducted

atultra-tracelevels.WithagreementoftheSFEI-ASCProjectManagerandQAOinconsultation

withtheLaboratory,resultsforsamplegroupswithdataoutsideofMQOsmaybeflagged

ratherthanreanalyzed,toindicatethegreateruncertaintyinthequantitationofthosedata.

ResultsonindividualanalytesthataregreatlyoutsidethetargetMQOrange(e.g.z-scores>2)

willbecensoredasneededratherthansubjectedtorepeatedanalysis.Reports,graphs,tables,

orsummarystatisticsgeneratedfromdatasetswithcensoreddatashouldnotetheirexclusion

orotherhandling.

Repeatedanalysismaynotfixanyissuesbutratherjustmaskvariability,creatingafalse

impressionofthequantitativecertaintyofresults.Contaminationofmethodblankscan

sometimesrepresentatemporarysourceofcontamination,andflaggingresultsofbatchesin

whichcontaminationisfoundinblanksisappropriate.Sampleresultsinbatcheswithdetected

blankcontaminationwillbeflagged(forfieldsampleswithanalyteconcentration>3xthose

foundinmethodblanks,“IP”whenappliedbythereportinglab,or“VIP”ifaddedlaterbySFEI-

ASC)orcensored(forresults<3xthoseinblanks)bySFEI-ASC,butdatausersshouldbeaware

ofthepossibleinfluenceofsporadiccontaminationinotherbatchesanalyzedaroundthesame

time,particularlyforsampleswithlowconcentrationssimilartothoseinblanks.

SimilaranalogiescanbemadewithfailuresofprecisionoraccuracyQCmeasurements.

Individualfailuresmayfallwithintherangeofthetruevarianceinthemeasurement,e.g.NIST

acceptancerangesaresometimesinexcessof±50%ofthemeanvalues,andwhilereporting

onlysuccessfulreanalysisbatchesmayappeartoproducemoreconsistentandcertainresults,

withoutfundamentalchangestotheanalyticalprocess,theunderlyinguncertaintymayonly

havebeenmasked/censoredratherthantrulyreducedforthereportedfieldsamples.Thisis

nottosaythatreanalysesareneverwarrantedordesirable,butrathertounderscorethat

improvedresultsonQCmeasurements,whichcansometimesbeachievedsimplybyrepeat

analysisanddiscardingpreviousfailedresults,shouldnotbeconfusedwithimproved

measurements,whichareonlyachievedbymakingrealsubstantivechangestothesampling

and/oranalyticalmethods.Ifreanalysisistobeattempted,itisthereforeimperativethatthe

ProjectManagerandQAOworkinconsultationwithlaboratorystafftoidentifyandchangethe

factorsthatmayhaveledtoMQOdeviances,ratherthansimplyrepeattheanalysisuntilthe

QCpasses.ForMQOdeviations(z-scoreorp-score>1)forwhichcausesarenotidentifiedand

thatarenotfixedbycorrectiveactions,fieldsampleresultsmaybequalified,orcensoredif

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grosslydeviating(z-scoreorp-score>2).TheQCdatausedfordeterminationofflaggingis

subjecttotheavailabilityofdataonvariousQCsampletypesandtheprofessionaljudgmentof

theQAO.DecisionswillbedocumentedinanarrativesummaryoftheQAreview.Where

possible,dataforflaggingrecoveryshouldbe1)inasimilarmatrixassamples,2)with

externallyvalidatedexpectedvalues,3)inaquantitativerange,and4)inasimilar

concentrationrangeasfieldsamples.Thusforevaluatingrecovery,theorderofpreferenceis

generallyCRM>LRM>MS>LCS,withexceptionsandchangesinpreferencemadeforfactorssuch

asnon-certifiedvalues,certifiedvalueswithwideuncertaintybands,andconcentrations

greatlydifferentfromthoseinfieldsamples.Similarly,forevaluationandflaggingoflab

precision,QCsamplesshouldbe1)inthesamematrixasfieldsamples,2)isolatelabvariation

fromothercauses,3)inaquantitativerange,and4)inasimilarconcentrationrangeasfield

samples,whereavailable.Forevaluatingprecisionthen,thepreferredsampletypesfor

replicatesare:lab>field>MS~CRM>LCS,againwithexceptionsmadedependingonthe

availablesampletypes,theirinherentvariability,concentrationranges,andotherfactors.Flags

appliedtodataaretobeselectedfromamongthoseapprovedbyCEDENappropriateforthe

specificMQOfailure(e.g.,“GBC-CRManalyterecoverynotwithincontrollimits”toaCRM

resultoutsideofacceptancetargets,“IU-PercentRecoveryexceedslaboratorycontrollimit”

forfieldsamplesreportedatthetimeifthedeviationappearsrandom,or“LHB-Result

positivelybiased,flaggedbylab”,ifresultsappearsystematicallyhighbiased).Thebasesfor

anyflaggingoffieldsampleswillbedocumentedinanarrativesummaryoftheQAreview.

TheQA/QCrequirementspresentedintheprecedingsectionsareintendedtoprovidea

commonfoundationforeachlaboratory’sprotocols;theresultantQCdatawillenable

assessmentofthecomparabilityanduncertaintyofresultsgeneratedbydifferentlaboratories

andanalyticalprocedures.ItshouldbenotedthattheQCrequirementsspecifiedinthisplan

representtheminimumrequirementsforanygivenanalyticalmethod;labsarefreetoperform

additionalQCinaccordancewiththeirstandardpractices.

InadditiontoperformanceonrequiredQCmeasuresandsamples(i.e.MDLs,blanks,matrix

spikes,CRM,replicates),dataarealsoexaminedforinternalandexternalconsistencytoensure

thatreportedvaluesarerealisticandrepresentativeforthelocationsandmatricesofcollected

samples.Thisreviewmayincludebutisnotlimitedto:

1. Comparisonofreportedvaluestothosefrompreviousyearsforthesamelocationsand

matrices,whereavailable–largedifferencesfrompreviouslyreportedvaluesmaynot

necessarilyindicateanalyticalissuesandmaysimplyreflectnaturalspatialandtemporal

variabilityoftheecosystem.

2. Comparisonofreportedvaluestothoseinthepublishedliterature,whereavailable–

differencesfromotherregionsand/orspeciesmaymerelyindicatedifferencesinresident

speciesandecosystemstructure,butverylarge(e.g.2-3ordersofmagnitude)differences

maysometimeshelpidentifyerrorsinanalysisorreporting(e.g.unitconversions).

3. Internalchecksofrelativeanalyteabundance–variationsinconcentrationsofone

compoundorisomerinaclassofchemicalcontaminantsareoftentightlylinkedtothoseof

relatedcompounds,suchasacompoundanditsdegradationproductsormanufacturing

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byproducts,orvariouscongenersinacommercialmixture.Deviationsintheserelative

abundancescansometimesindicatematrixinterferencesorotheranalyticalproblems,

althoughcareshouldbetakentonotdisregardresultsthatmightrevealatypicalsources

and/orecosystemprocesses.

AtthecompletionoftheQAreviewbytheQAO,resultsareassignedacompliancecodeonan

individualrecordlevel.SeeTable17.1forcompliancecodes.Dataarefurtherassignedabatch

verificationcodeonabatchlevel.SeeTable.17.2batchverificationcodes.Resultsfromthe

datareviewwillbesummarizedintheannualQAReport.

Table17.1. ComplianceCodes.

DataCompliance Name DataCompliance Code Compliant Com Do Not Use DNU Estimated Est Historical Hist Not Applicable NA Not Recorded NR Pending QA review Pend Qualified Qual Qualified Historic QualH Rejected Rej Screening Scr

Table17.2. BatchVerificationCodes.

BatchVerification Name BatchVerification Code Alternate Level Validation VAP Alternate Level Validation, Incomplete QC VAP,VI

Alternate Level Validation, Incomplete QC, Flagged by QAO

VAP,VQI

Cursory Verification, Data Rejected - EPA Flag, Flagged by QAO

VAC,VR

Cursory Verification, Minor Deviations, Flagged by QAO

VAC,VMD

Cursory Verification, Minor Deviations, Incomplete QC, Flagged by QAO

VAC,VMD,VQI

Cursory Verificaton VAC Cursory Verificaton, Incomplete QC, Flagged by QAO

VAC,VQI

Cursory Verificaton/Validation VLC Cursory Verificaton/Validation, Incomplete QC, Flagged by QAO

VLC,VQI

Cursory Verificaton/Validation, Minor Deviations, Flagged by QAO

VLC,VMD

Cursory Verificaton/Validation, Minor Deviations, Incomplete QC, Flagged by QAO

VLC,VMD,VQI

Data Rejected - EPA Flag, Flagged by QAO VR

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Full Verification VAF Full Verification, Incomplete QC, Flagged by QAO VAF,VQI

Full Verification, Minor Deviations, Flagged by QAO

VAF,VMD

Full Verification/Validation VLF Incomplete QC, Flagged by QAO VQI Incomplete QC, Temporary Verificaton, Flagged by QAO

VQI,VTC

Minor Deviations, Flagged by QAO VMD No QC, Flagged by QAO VQN Not Applicable NA Not Recorded NR Temporary Verification VTC

18. Reconciliation with User Requirements

AlldataarereviewedbytheQAOtodetermineiftheresultshavemettheDeltaRMPMQOsof

completeness,sensitivity,precision,andaccuracy.Limitationsofthedata,includinguncertainty

ofvalidateddata,arereportedtothedatausersbyaQAcodeorqualifier.TheDeltaRMPhas

adoptedtheCaliforniaDataExchangeNetwork’s(CEDEN)standardlistofcodestoflagdataat

theresultandanalyticalbatchlevel;theDeltaRMPusesasubsetoftheavailablecodestoflag

variousQCissuesasneeded.TheQAReportdescribesnon-conformanceswithQAPP

specifications.ThesefindingsshouldalsobeincludedinthedataitselfinQAcodes,result

qualifiercodes,compliancecodes,batchverificationcodes,andcommentfields,sothatalldata

userswillbeinformedofthequalityofthedata.

ThedatawillbestoredandmaintainedintheRegionalDataCenterdatabasestructureandwill

followCEDEN’sbusinessrules.

Measurementqualityobjectiveslistedpreviously(Section4)establishtargetstoberoutinely

achievedbytheanalyticallaboratory.However,itisuncertainwhetherobtaineddata,even

whenmeetingallstatedMQOs,willbesufficienttoanswertheDeltaRMPmanagement

questionswithsufficientcertainty,astherelativecontributionsofenvironmentalvariabilityand

analyticaluncertaintytooveralluncertainty(e.g.foruseinmodeling,comparisonsto

guidelines,orotherfunctions)cannotbeknownaprioribeforesufficientdatahavebeencollected.However,asDeltaRMPstudiesproceed,theabilityofcollecteddatatoanswerthese

managementquestionsshouldbeperiodicallyre-evaluatedforstudydesignandbudget

planninginsubsequentyears.

19. References

CaliforniaStateWaterResourcesControlBoard(SWRCB).2008.QualityAssuranceProgram

PlanfortheStateofCalifornia'sSurfaceWaterAmbientMonitoringProgram(SWAMP).

PreparedbytheSurfaceWaterAmbientMonitoringProgramQualityAssuranceTeam

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QualityAssuranceResearchGroup.Updated.

http://www.swrcb.ca.gov/water_issues/programs/swamp/docs/mqo/15_acute_toxicity.pdf

CaliforniaStateWaterResourcesControlBoard(SWRCB).QualityControlandSampleHandling

Tables.UpdatedQualityControlandSampleHandlingTables.

http://www.waterboards.ca.gov/water_issues/programs/swamp/mqo.shtml

CentralValleyWaterBoard.1998.TheWaterQualityControlPlan(BasinPlan)fortheCalifornia

RegionalWaterQualityControlBoard,CentralValleyRegion.TheSacramentoRiverBasin

andtheSanJoaquinRiverBasin.FourthEdition.CaliforniaRegionalWaterQualityControl

Board,CentralValleyRegion.

CentralValleyWaterBoard.2007.AmendmenttotheWaterQualityControlPlanforthe

SacramentoRiverandtheSanJoaquinRiverBasinsfortheControlofChlorpyrifosand

DiazinonRunoffIntotheSacramento-SanJoaquinDelta.CaliforniaRegionalWaterQuality

ControlBoard,CentralValleyRegion,ResolutionNo.R5-2006-0061.

CentralValleyWaterBoard.2011.AmendmentstotheWaterQualityControlPlanforthe

SacramentoRiverandSanJoaquinRiverBasinsfortheControlofMethylmercuryandTotal

MercuryintheSacramento-SanJoaquinRiverDeltaEstuary(Attachment1toResolution

No.R5-2010-0043).

CEDEN.2014.CEDEN-CaliforniaEnvironmentalDataExchangeNetwork.DataTemplates

Templates,andDocumentation.Retrievedfrom

http://www.ceden.org/ceden_datatemplates.shtml

EPA.2000.WaterQualityCriteriaforPriorityToxicPollutantsforCaliforniaInlandSurface

Waters,EnclosedBaysandEstuaries.40CFRPart131,U.S.EnvironmentalProtection

Agency.

EPA.2002a.Short-termMethodsforEstimatingtheChronicToxicityofEffluentsandReceiving

WaterstoFreshwaterOrganisms.FourthEdition.EPA-821-R-02-013

EPA.2002b.MethodsforMeasuringtheAcuteToxicityofEffluentsandReceivingWatersto

FreshwaterandMarineOrganisms.FifthEdition.EPA-821-R-02-012

EPA.2013.BinClassificationforFilteredSystems.40CFRPart141.710,U.S.Environmental

ProtectionAgency.

EPA.2015a.AquaticLifeBenchmarksforPesticideRegistration.URL:

http://www2.epa.gov/pesticide-science-and-assessing-pesticide-risks/aquatic-life-

benchmarks-pesticide-registration#benchmarks.AccessedonJuly8,2016.

EPA.2015b.NationalRecommendedWaterQualityCriteria-AquaticLifeCriteriaTable.URL:

http://www2.epa.gov/wqc/national-recommended-water-quality-criteria-aquatic-life-

criteria-table.AccessedonNovember23,2015.

Keith,L.H.1991.EnvironmentalSamplingandAnalysis:APracticalGuide.LewisPublishers.

Chelsea,MI.

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Keith,L.H.,Crummett,W.,Deegan,J.,Libby,R.A.,Taylor,J.K.andWentler,G.1983.Principles

ofEnvironmentalAnalysis.AnalyticalChemistry55(14):2210-2218.

Luo,Y.,Deng,X.,Budd,R.,Starner,K.andEnsminger,M.2013.MethodologyforPrioritizing

PesticidesforSurfaceWaterMonitoringinAgriculturalandUrbanAreas

(http://www.cdpr.ca.gov/docs/emon/surfwtr/monitoring_methods.htm).California

DepartmentofPesticideRegulation,Sacramento,CA.

Taylor,J.1987.QualityAssuranceofChemicalMeasurements.LewisPublishers.Chelsea,MI.

Stanley,T.W,andVerner,S.S.1985.TheU.S.EnvironmentalProtectionAgency’sQuality

AssuranceProgram.In:Taylor,J.K.,andStanley,T.W.(eds.)QualityAssuranceof

EnvironmentalMeasurements.ASTMSTP867.AmericanSocietyforTestingandMaterials.

pp.12-19.

AppendixA

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20. Appendices

20.1. AppendixA.ManagementQuestions

Type

ManagementQuestions

StatusandTrends

Isthereaproblemoraretheresignsofaproblem?

a. Iswaterqualitycurrently,ortrendingtowards,adversely

affectingbeneficialusesoftheDelta?

b. Whichconstituentsmaybeimpairingbeneficialusesin

subregionsoftheDelta?

c. Aretrendssimilarordifferentacrossdifferentsubregionsof

theDelta?

Sources,Pathways,Loadings,

andProcesses

Whichsourcesandprocessesaremostimportanttounderstand

andquantify?

a. Whichsources,pathways,loadings,andprocesses(e.g.,

transformations,bioaccumulation)contributemostto

identifiedproblems?

b. Whatisthemagnitudeofeachsourceand/orpathway(e.g.,

municipalwastewater,atmosphericdeposition)?

c. Whatarethemagnitudesofinternalsourcesand/orpathways

(e.g.benthicflux)andsinksintheDelta?

ForecastingWaterQuality

UnderDifferent

ManagementScenarios

a. Howdoambientwaterqualityconditionsrespondtodifferent

managementscenarios

b. WhatconstituentloadscantheDeltaassimilatewithout

impairmentofbeneficialuses?

c. WhatisthelikelihoodthattheDeltawillbewaterquality-

impairedinthefuture?

EffectivenessTracking

a. Arewaterqualityconditionsimprovingasaresultof

managementactionssuchthatbeneficialuseswillbemet?

b. Areloadingschangingasaresultofmanagementactions?

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20.2. AppendixB.AssessmentQuestions

DeltaRMPassessmentquestionsformercury,pesticides,andpathogens.Questionshighlighted

inyellowarethehighestpriorityforinitialstudies.

Type Core

Management

Questions Mercury Pesticides Pathogens

Status&

Trends

Istherea

problemorare

theresignsofa

problem? a. Iswater

quality

currently,or

trending

towards,

adversely

affecting

beneficial

usesofthe

Delta?

b. Which

constituents

maybe

impairing

beneficial

usesin

subregionsof

theDelta?

c. Aretrendssimilaror

different

across

different

subregionsof

theDelta?

1. Whatarethestatusand

trendsinambient

concentrationsoftotal

mercuryand

methylmercury(MeHg)in

fish,water,andsediment,

particularlyinsubareas

likelytobeaffectedby

majorsourcesornew

sources(e.g.,large-scale

restorationprojects)?

A. Aretrendsovertimein

MeHginsportfish

similarordifferent

amongDeltasubareas?

B. Aretrendsovertimein

MeHginwatersimilar

ordifferentamong

Deltasubareas?

1. Towhatextentdopesticides

contributetoobserved

toxicityintheDelta?

1.1. Whichpesticidesor

degradateshavethehighest

potentialtobecausing

toxicityintheDeltaand

thereforeshouldbethe

priorityformonitoringand

management?

A. Ifsamplesaretoxic,do

detectedpesticidesexplain

thetoxicity?

B. Ifsamplesarenottoxic,do

detectedpesticide

concentrationsexceed

otherthresholdsofconcern

(e.g.,waterquality

objectivesorOfficeof

PesticideProgramsaquatic

toxicitybenchmarks)?

1.2. Whatarethespatialand

temporalextentsoflethal

andsublethalaquaticand

sedimenttoxicityobserved

intheDelta?

A. Doaquaticorsediment

toxicitytestsattargeted

sitesindicateatoxic

response?

B. IfanswertoAisyes,whichothertoxicityindicator(s)

shouldguidemonitoring

andmanagementof

pesticidesinYears2+?

2. Whatarethe

spatial/temporaldistributions

ofconcentrationsofcurrently

usedpesticidesidentifiedas

likelycausesofobserved

toxicity?

2.1. Whichpesticideshavethe

highestriskpotential

1. Arecurrentpathogen

levelssupportiveofthe

municipaldrinkingwater

qualitybeneficialuseas

describedintheBasin

Plan?

A. Arethecurrent

pathogenlevelsfor

eachDeltawater

intakeandthose

immediatelyupstream

(i.e.,SacramentoArea)

differentthanthe

previousLT2

sampling?Areany

drinkingwaterintakes

reclassifiedintoa

higherbinlevel?

B. AreBasinPlantrigger

valuesexceeded?

AppendixB

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Type Core

Management


(basedonDPR’srisk

prioritizationmodel16)and

shouldbeincludedin

chemicalanalyses?

A. Isthelistofpesticides

includedinUSGS

pesticidescansufficient

forDeltaRMP

monitoringdesign?

B. Aremethodsavailableto

monitorpesticideswith

high-riskpotentialnot

includedinUSGS

pesticidescan?

2.2. Howdoconcentrationsofthepesticideswiththe

highestriskpotentialvary

seasonallyandspatially?

Sources,

Pathways,

Loadings&

Processes

Whichsources

andprocesses

aremost

importantto

understandand

quantify? a. Which

sources,

pathways,

loadings,and

processes

(e.g.,

transformatio

ns,

bioaccumulati

on)contribute

mostto

identified

problems?

b. Whatisthe

magnitudeof

eachsource

1. Whichsources,pathways

andprocessescontribute

mosttoobservedlevelsof

methylmercuryinfish?

A. Whataretheloadsfrom

tributariestotheDelta

(measuredatthepoint

wheretributariescross

theboundaryofthe

legalDelta)?

B. Howdointernalsources

andprocessesinfluence

methylmercurylevelsin

fishintheDelta?

C. Howdocurrently

uncontrollablesources

(e.g.,atmospheric

deposition,bothas

directdepositionto

Deltasurfacewaters

andasacontributionto

nonpointrunoff)

influence

methylmercurylevelsin

1. Whataretheprincipal

sourcesandpathways

responsibleforaquaticand

sedimenttoxicityobservedin

theDelta?

2. Whatarethefatesof

prioritizedpesticidesand

degradatesinthe

environment?

2.1. Dophysical/chemical

propertiesofpriority

pesticides,applicationrates

andprocesses,andambient

conditionsinfluencethe

degreeoftoxicityobserved?

3. Whatarethe

spatial/temporalusepatterns

ofprioritypesticides?

1. Cananychangesinbinlevel

17beattributedtoan

identifiableevent,

condition,orchangesina

source?

A. Whatistheinfluenceof

sourcesonpathogen

levelsatdrinkingwater

intakes?

B. Whatistheviabilityand

infectiousnessof

pathogensatdrinking

waterintakes?

C. Aretherenew

dischargesorchangesin

sourcesorconditions

thatcouldexplainthe

changeinbinlevel

comparedtoprevious

LT2monitoring?

2. Whatarethefactors

affectingdecayandgrowth

ratesandcantheybe

quantifiedand

16 http://www.cdpr.ca.gov/docs/emon/pubs/ehapreps/analysis_memos/prioritization_report_2.pdf 17EPAhasdevelopedtheLongTerm2EnhancedSurfaceWaterTreatmentRule(LT2rule),whichclassifiesfilteredwater

systemsintooneoffourtreatmentcategories(bins)basedontheirmonitoringresultsforCryptosporidium.Mostsystemsare

expectedtobeclassifiedinthelowestbinandwillfacenoadditionalrequirements.Systemsclassifiedinhigherbinsmust

provideadditionalwatertreatmenttofurtherreduceCryptosporidiumlevelsby90to99.7percent(1.0to2.5-log),depending

onthebin.From:RuleFactSheet-LongTerm2EnhancedSurfaceWaterTreatmentRule(EPA2005).

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Type Core

Management


and/or

pathway(e.g.,

municipal

wastewater,

atmospheric

deposition)?

c. Whatarethe

magnitudesof

internal

sources

and/or

pathways

(e.g.benthic

flux)andsinks

intheDelta?

fishintheDelta? characterizedforthe

purposeofmodeling?

Forecasting

Scenarios

a. Howdoambient

waterquality

conditions

respondto

different

management

scenarios

b. What

constituent

loadscanthe

Delta

assimilate

without

impairmentof

beneficial

uses?

c. Whatisthe

likelihoodthat

theDeltawill

bewater

quality-

impairedin

thefuture?

1. Whatwillbetheeffectsof

in-progressandplanned

sourcecontrols,restoration

projects,andwater

managementchangeson

ambientmethylmercury

concentrationsinfishinthe

Delta?

1. Howdopesticideconcentrationsrespondto

differentmanagement

scenarios?

2. Whatpesticideloadscanthe

Deltaassimilatewithout

exceedingwaterquality

criteriaestablishedtoprotect

beneficialuses?

3. Howwillclimatechange

affectconcentrationsand/or

loadingsofpesticidesand

impactstoaquaticspecies?

1. Whatistheeffectof

sourcecontrolson

pathogenlevelsat

drinkingwaterintakes?

2. Howwillproposedrestorationprojects,

wateroperations,and

futureurbangrowth

affectmunicipaldrinking

waterintakebinlevels?

Effectiveness

Tracking

a. Arewaterquality

conditions

improvingas

aresultof

management

actionssuch

thatbeneficial

useswillbe

met?

[none] 1. Arepesticide-related

toxicityimpacts

decreasingovertime?

[none]

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Type Core

Management


b. Areloadingschangingasa

resultof

management

actions?

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20.3. AppendixC.DeltaRMPMonitoringElements

Pesticides

Monthlysamplingatfivesites,whichalsocapturetargetedevents.Targetedevents(n=

5/year):WetWeather:(1)1stseasonalflush(WaterYear),(2)Significantwinterstorm;Dry

weather:(1)EarlySpring,(2)Latespring/earlysummerirrigationseason,(3)Latesummer

irrigationseason.Chemicalanalysesandtoxicitytestingonallsamples.Testspecies

(endpoints):(1)Selenastrumcapricornutum(growth)(2)Ceriodaphniadubia(survivalandreproduction),and(3)Pimephalespromelas(larvalsurvivalandgrowth).Chemistry:pesticide

scan(USGS),totalsuspendedsolids,dissolvedorganiccarbon(DOC)andparticulateorganic

carbon(POC),hardness,anddissolvedcopperanalysis.Pesticide-focusedToxicityIdentification

Evaluations(TIEs)forasubsetofsampleswith>50%ofthemeasuredendpoint;tobedecided

real-timebyaTIEsubcommittee.

Mercury

SportFish

Annualsamplingatsixfixedsitesbeginningin2016.Indicatorofprimaryinterestis

methylmercuryinmusclefilletof350-mmlargemouthbass(orsimilarpredatorspecies).Sites

arelocatedtorepresentdifferentsubareasoftheDeltaandtolinkwithwatermonitoring.

Water

Quarterlysamplingatfivesitesthatalignwithsportfishmonitoringsites.Indicatorofprimary

interestistotalmethylmercuryinwater.

ImportantancillaryparametersincludetotalanddissolvedtotalHg,chlorophylla,DOC,suspendedsedimentconcentrations,andvolatilesuspendedsolids.

Pathogens

Monthlysamplingforatwo-yearspecialstudycharacterizingpathogenlevels(Cryptosporidium

andGiardialamblia)toaddresstheobjectivesofthePathogenSpecialStudyrequiredbytheCentralValleyDrinkingWaterPolicyBasinPlanAmendment.Thestudyincludesmonitoringat

ambientlocationsthroughouttheDelta.Thesamplingisaddedtotheroutinemonthlysampling

effortoftheDepartmentofWaterResources(DWR)MunicipalWaterQualityInvestigations

(MWQI).TheDeltaRMPcontributesrequiredadditionallaboratoryanalyses,data

management,andreporting.

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20.4. AppendixD.ListofSOPs

ThefollowingSOPs,manuals,andmethodreferencedocumentswillbemadeavailable

onCDbyrequestorcanbedownloadedfromtheSFEI-ASCGoogleDrive.

Field

USGS

− NationalFieldManualfortheCollectionofWater-QualityData − CollectionofPyrethroidsinWaterandSedimentMatrices:DevelopmentandValidationofa

StandardOperatingProcedure

MPSL

− MPSLFieldSOPv1.1

− MPSL-102aSamplingMarineandFreshwaterBivalves,FishandCrabsforTraceMetaland

SyntheticOrganicAnalysis − Lowlevelmercury(USGSNFMA5.6.4.B)

MWQI

− MWQIProgramFieldManual

− MWQIProgramFieldManual,AppendixA–DeltaRMPPathogenStudyCryptosporidiumand

GiardiaSampling

ChemicalAnalysis

USGS

− DeterminationofElementsinNatural-Water,Biota,Sediment,andSoilSamplesUsing

Collision/ReactionCellInductivelyCoupledPlasma–MassSpectrometry(USGSTM-5-B1) − MethodsofAnalysis—DeterminationofPyrethroidInsecticidesinWaterandSedimentUsingGas

Chromatography/MassSpectrometry(USGSTM-5-C2) − Analysisoftheherbicidediuron,threediurondegradates,andsixneonicotinoidinsecticidesin

water—MethoddetailsandapplicationtotwoGeorgiastreams(USGSSIR2012-5026) − AMulti-residueMethodfortheAnalysisofPesticidesandPesticideDegradatesinWaterUsing

HLBSolid-phaseExtractionandGasChromatography–IonTrapMassSpectrometry − WATEREXTRACTIONforGCMSanalysisusingHLBcartridges − SuspendedsedimentonFilterPaperEXTRACTIONforGCMSanalysis − WATEREXTRACTIONforLCMSMSanalysisusingHLBcartridges − ProceduresforProcessingSamplesforAnalysisofDissolvedOrganicCarbonandOrganic

ParticulateCarbon − DeterminationofCarbonandNitrogeninSedimentsandParticulatesofEstuarine/Coastal

WatersUsingElementalAnalysis(EPA440.0) − MethodsofAnalysisbytheU.S.GeologicalSurveyNationalWaterQualityLaboratory-

DeterminationofDissolvedOrganicCarbonbyuv-promotedPersulfateOxidationandInfrared

Spectrometry(USGSTestMethodO-1122-92)

MPSL

− MercuryinSolidsandSolutionsbyThermalDecomposition,Amalgamation,andAtomic

AbsorptionSpectrophotometry(EPA7473)

− MercuryinWaterbyOxidation,PurgeandTrap,andColdVaporAtomicFluorescence

Spectrometry(EPA1631,RevisionE)

− MethylMercuryinWaterbyDistillation,AqueousEthylation,PurgeandTrap,andColdVapor

AtomicFluorescenceSpectrometry(EPA1630)

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− MPSL-101 Sample Container Preparation for Organics and Trace Metals, Including Mercury and Methylmercury

− MPSL-104SampleReceiptandCheck-In

− SM2540DSolids

CDFW-WPCL

− InVitroDeterminationofChlorophyllsa,b,c1+c2andPheopigmentsinMarineAndFreshwater

AlgaebyVisibleSpectrophotometry(EPA446.0)

− TOTALORGANICCARBONinWater(EPA415.1)

− WPCL-AB-001SampleCustody,Receipt,andStorage

− WPCL-QA-050ProtocolforCorrectiveActionProcedures

ToxicityTesting

UCD-AHPL

− InitiationofSelenastrumcapricornutum96-HourChronicToxicityTest(4thEdition)(SOP1-1)

− InitiationofCeriodaphniadubiaChronicToxicityTest(4thEdition)(SOP1-2)− InitiationofPimephalespromelas(FatheadMinnow)ChronicToxicityTest(4thEdition)(SOP1-3)

− InitiationofHyalellaaztecaAcute96-hourWaterColumnToxicityTest(SOP1-6)

− ProtocolforSampleReceivingandStorage–DeltaRMPTesting(SOP12-7)

ToxicityIdentificationEvaluations(TIEs)

UCD-AHPL

− ProtocolforMakinga5ppmSolutionofPBOandSpikingitintoSampleWaters(SOP7-1)

− C8SolidPhaseExtraction(SOP7-2)

− C8ColumnElutionforPhaseITIEs(SOP7-3)

− C8ColumnElutionforPhaseIITIEs(SOP7-4)

− AmendmentofWaterSampleswithEDTAandNa2S2O3(SOP7-9)

− pHAdjustmentstopH3andpH11(SOP7-10)

− Aeration(Volatile/SurfactantStripping)(SOP7-11)

ToxicityTesting-WaterQualityMeasurements

UCD-AHPL

− AnalysisforTotalWaterHardness(SOP6-1)

− AnalysisforAmmoniaNitrogen(mg/L)(SOP6-3)

− AnalysisforAlkalinity(SOP6-5)

− UseofYSIModel33ElectricalConductivityMeter(SOP8-7)

− OperationofBeckman12pH/ISEMeter(SOP8-8)

− ProtocolfortheYSIModel58DissolvedOxygenMeter(SOP8-9)

SWAMPDocumentation

− SWAMPToxicityTemplateDocumentation

− SWAMPToxicityTemplate

− SWAMPSampleHandling,MeasurementQualityObjectives,andCorrectiveActionTables

PathogenAnalysis

BioVir

− EPAMethod1622,1623,1623.1CryptosporidiumandGiardiainWaterbyFiltration/IMS/FA:

SampleFiltration(SOPX.C.2.a)

− EPAMethod1622,1623and1623.1CryptosporidiumandGiardiainWaterbyFiltration/IMS/FA:

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ElutionandConcentration(SOPX.C.2.b)


ImmunomagneticSeparation(IMS)(SOPX.C.2.c)


SlideStainingProcedure(SOPX.C.2.d)


SlideExamination(SOPX.C.2.e)

Eurofins

− EPAMethod1622/1623(Micro-SOP3404)

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20.5. AppendixE.ExampleFieldSheets

November 2013 1 SW Form version 9.0

COMPILED BY: ___________________________ CHECKED BY: ________________________________ LOGGED INTO NWIS BY: _____________________________

SAMPLING INFORMATION

Parameter Pcode Value Information

Sampler Type 84164 see last page for proper codes— consider type of sampler and material

Sampler ID:

Sampling Method 82398 10 EWI; 20 EDI; 30 single vertical; 40 multiple vertical; other ______________________

BAG SAMPLER EFFICIENCY TEST

Sampler bottle/bag material 84182 Plastic Bag (11) Teflon® Bag(12) Glass Bottle(20) Plastic Bottle (21) Teflon® Bottle (22) other (30)

Test Duration Sampler Collected Water

(seconds)

Sample Volume Collected (milliliters)

Sampler Nozzle material 72219 plastic (2) Teflon® (3) Brass (1) 1

Sampler Nozzle Diameter 72220 3/16” (3) 1/4” (4) 5/16” (5) 2

Sampler Transit Rate 50015 feet/second 3

Velocity to Calculate Isokinetic transit rate

72196 feet/second Mean (72217) (72218)

Depth to Calculate Isokinetic transit rate 72195 feet . Bag Sampler Efficiency % (See last page)

Splitter Type 84171 See last page for codes ____________ Splitter ID: Hydrologic Condition N/A A Not Determined; 4 Stable, low stage; 5 Falling stage; 6 Stable, high stage; 7 Peak stage; 8 Rising stage; 9 Stable, normal

stage Observations [Codes: 0=none; 1=mild; 2=moderate; 3=serious; 4=extreme]

Oil-grease (01300) ___ Detergent suds (01305) ___ Floating garbage (01320) ___ Floating algae mats (01325) ___ Floating debris (01345) ___ Turbidity (01350) ___ Atm. Odor (01330) ___ Fish kill (01340) ___ Gas Bubbles (01310) ___ Sewage Solids (01335) ___ Floating Vegetation (84178) ___ Ice Cover(01355) ___

Station No. ____________________ U. S. GEOLOGICAL SURVEY SURFACE-WATER QUALITY FIELD NOTES NWIS Record No. ____________________

Station No. ________________________ Station Name ________________________________________________ Field ID _________________ Sample Date _____________ Mean Sample Time _______ Time Datum ______ (eg. EST, EDT, UTC) End Date _________ End Time _______ *Sample Medium: WS WSQ OAQ *Sample Type: 9 (regular) 7 (replicate) 2 (blank) 1 (spike) __________ *Sample Purpose (71999): 10 (routine) 15 (NAWQA) 20 (NASQAN) 25 (NMN) 30 (Benchmark) _____________________ *Purpose of Site Visit (50280): 1001 (fixed-frequency SW) 1003 (extreme high flow SW) 1004 (extreme low flow SW) 1098 (NAWQA QC) ______ QC Samples Collected? Y N Blank Replicate Spike Other_______________________ Project No. __________________________________________ Project Name_______________________________________________________ Sampling Team ______________________________________ Team Lead Signature ________________________________ Date ___________ START TIME __________ GAGE HT _______ TIME __________ GHT ______ TIME __________ GHT ______ TIME __________ GHT ______ END TIME __________ GHT _______

* see last page for additional codes

FIELD MEASUREMENTS

Property Parm Code

Method Code http://water.usgs.gov/usgs/owq/Forms/

Fieldmeasure-ment_parametersmethods.doc Result Units

Remark Code

Value Quali-

fier

Null Value Quali-

fier NWIS Result-Level Comments Gage Height 00065 ft Discharge, instantaneous

00061 cfs

Temperature, Air 00020 THM04 (Thermistor) THM05 (Thermometer)

˚C

Temperature, Water 00010 THM01 (Thermistor) ˚C Specific Conductance 00095 SC001 (Contacting Sensor) PS/cm Dissolved Oxygen 00300 LUMIN (Luminescent)

MEMBR (Amperometric) SPC10 (Spectrophotometric)

mg/L

Barometric Pressure 00025 BAROM (Barometer) mm Hg pH 00400 PROBE (Electrode) units Alkalinity, filtrd, incr. Alkalinity, filtrd, Gran

39086 29802

TT061 (Digital Titrator) TT062 (Buret) TT056 (Digital Titrator) TT057 (Buret)

mg/L

Carbonate, filtrd, incr. Carbonate, filtrd, Gran

00452 63788

ASM01(Digital Titrator) ASM02(Buret) ASM03(Digital Titrator) ASM04(Buret)

mg/L

Bicarbonate, filtrd, incr. Bicarbonate, filtrd, Gran

00453 63786


mg/L

Hydroxide, filtrd, incr. Hydroxide, filtrd, Gran

71834 29800


mg/L

Turbidity [see attachment for codes and units]

Attach ASR and WatList

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AppendixE


AppendixE


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AppendixF


20.1. AppendixF.ExampleforChainofCustodyForm