[Biochemistry 1] nitroprusside reaction

NITROPRUSSIDE REACTION

Group’s Member:ATIKAH (F02111045)

DESY INDRA WAHYUNI (F02111033)

Sodium Nitroprusside• Nitroprusside is a complex anion that

contain a centered octahedral iron, surrounded by five cianide ligands which firmly bond and one ligand of linier nitric oxide.

• Sodium nitroprusside test is performed to test for the presence of proteins 'cysteine' in the test solution.

Cysteine is found in most high-protein foods, including:• Animal

sources: pork, poultry, eggs, dairy• Plant sources: red peppers,

garlic, onions,broccoli, brussels sprout, oats, granola, wheat germ, sprouted lentils.

Beside having the –COOH group, -NH2 group and R group, cysteine also has a free –SH group (sulfhydryl group ).If it reacted with sodium nitroprusside in an excess ammonia resulting a red-coloured complex.

Reaction: [Fe3+(CN)5NO]2- + NH3 + RSH ⟶ NH4+

+ [Fe2+ (CN)5NOSR]2-

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