Transcript
Bacteria From Natural Habitats
JR’s Belly Button & Dr. Mikel’s Leaf
Team 1D Members Include:Andrea TaylorNasrin Saber
Gina Blanchet-ParkerJose Rey Manaois
General Microbiology 260 / Lab D
Our Unknown Bacteria
1. Unknown Unknown (U/U): Belly button bacteria from JR Manaois
2. Known unknown: Leaf bacteria provided by Dr. Mikels
Our Investigation for Our“Unknown / Unknown”
After Gram staining the culture of the Belly ButtonBacteria and it’s colonies, we were able to determine the following:• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters
OUR PHOTO
• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters
Samples of other Gram stained S. epidermis bacteria
Our Investigation for Our“Unknown / Unknown”
After Gram staining the culture of the Belly ButtonBacteria and it’s colonies, we were able to determine the following:• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters
OUR PHOTO
• Gram + (Purple Stain)
• Shape: Cocci
• Arrangement: Clusters Samples of Gram stained S. cereus bacteria
Narrowing down the “Unknown Unknown”
(Belly Button)
• All G- bacteria• All bacteria shapes other
than coccus.• All bacteria not arranged in
clusters.
After Gram staining the culture of the Belly Button Bacteria and it’s colonies, we were able to eliminate all bacteria with the following attributes
Narrowing down the “Unknown Unknown”
(Leaf)
• All G- bacteria• All bacteria shapes other
than rods.• All bacteria not arranged at
random.
After Gram staining the culture of the Leaf Bacteria and it’s colonies, we were able to eliminate all bacteria with the following attributes
Which Bacteria Should Still Be Considered for the
“Unknown / Unknown”for the Leaf? 1. B. cereus• Info
2. B. subtilis• Info
2
0
2 2
0
1
2
3
Grow th Seen
0 = None1 = Slight
2 = Moderate3 - Abundant
PEA BA MSA -SALT
TSA
Type of Media Used
Gram + Bacteria Growth
S. epiderm idis
S. aureus
S. agalactiae
M. rosea
B. subtilis
B. cereus
Unknown/Unknown (BellyButton)
Known/Unknown (Leaf)
Which Bacteria Should Still Be Considered for the “Unknown / Belly
Button?1. S. epidermidis• Info Natural Flora found on Skin blah, blah, blah
2. S. aureus• Info
2
0
2 2
0
1
2
3
Grow th Seen
0 = None1 = Slight
2 = Moderate3 - Abundant
PEA BA MSA -SALT
TSA
Type of Media Used
Gram + Bacteria Growth
S. epiderm idis
S. aureus
S. agalactiae
M. rosea
B. subtilis
B. cereus
Unknown/Unknown (BellyButton)
Known/Unknown (Leaf)
A little something about our suspects for the Leaf
1. B. cereus• Info
2. B. subtilis• Info
A little something about our suspects for Belly Button
1. S. epidermis• Info
2. S. aureaus• Info
Growth TestsG- Bacteria on Variety of Media
3
1
2
0
0.5
1
1.5
2
2.5
3
3.5
PEA EMB SSA TSA
PEA = Phenylothanol BA = Blood AgerEMB = Eosin Methylene Blue (EMB)
SSA = Salmonella-Shigella AgarTSA = Trypticase Soy Agar
Growth0 = None1 = Slight
2 = Moderate3 - Abundant
P. vulgaris
K. pneum oniae
E. coli
S. m arcescens
E. aerogenes
P. aeruginosa
Belly Button (u/u)
Leaf (k/u)
Growth TestsG+ Bacteria on Variety of Media
0
1
PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS
S. epidermidis
S. aureus
S. agalactiae
M. rosea
B. subtil is
B. cereus
Unknown/Unknown (Bel ly Button)
Known/Unknown (Leaf)
Acid Key
0 = No
1 = Yes
Gas Key
0 = No Gas
1 = Yes, Gas
Media Used
Phenol Red Glucose (PRG)
Phenol Red Lactose (PRL)
Phenol Red Maltose (PRM)
Phenol Red Sucrose (PRS)
Phenol Red Arabinose (PRA)
Production of Carboxylic Acid & Gas Phenol Red Medias
Growth TestsG- Bacteria on Variety of Media
0
1
PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS
P. vulgaris
K. pneumoniae
E. col i
S. marcescens
E. aerogenes
P. aeruginosa
Acid Key
0 = No
1 = Yes
Production of Carboxylic Acid & Gas Phenol Red Medias
Gas Key
0 = No Gas
1 = Yes, Gas
Media Used
Phenol Red Glucose (PRG)
Phenol Red Lactose (PRL)
Phenol Red Maltose (PRM)
Phenol Red Sucrose (PRS)
Phenol Red Arabinose (PRA)
Gram – Bacteria on a Variety of Media
2 2
1
2
0
1
2
3
Grow th Seen
0 = None1 = Slight
2 = Moderate3 - Abundant
PEA BA MSA -SALT
MSA -FERM
TSA
Type of Media
S. epidermidis
S. aureus
S. agalactiae
M . rosea
B. subtilis
B . cereus
Unknown/Unknown (Belly Button)
Known/Unknown (Leaf)
PARAGRAPHS
PARAGRAPHS
top related