Ginas draft of presentation1

Bacteria From Natural Habitats

JR’s Belly Button & Dr. Mikel’s Leaf

Team 1D Members Include:Andrea TaylorNasrin Saber

Gina Blanchet-ParkerJose Rey Manaois

General Microbiology 260 / Lab D

Our Unknown Bacteria

1. Unknown Unknown (U/U): Belly button bacteria from JR Manaois

2. Known unknown: Leaf bacteria provided by Dr. Mikels

Our Investigation for Our“Unknown / Unknown”

After Gram staining the culture of the Belly ButtonBacteria and it’s colonies, we were able to determine the following:• Gram + (Purple Stain)

• Shape: Cocci

• Arrangement: Clusters

OUR PHOTO

• Gram + (Purple Stain)

• Shape: Cocci

• Arrangement: Clusters

Samples of other Gram stained S. epidermis bacteria

Our Investigation for Our“Unknown / Unknown”

After Gram staining the culture of the Belly ButtonBacteria and it’s colonies, we were able to determine the following:• Gram + (Purple Stain)

• Shape: Cocci

• Arrangement: Clusters

OUR PHOTO

• Gram + (Purple Stain)

• Shape: Cocci

• Arrangement: Clusters Samples of Gram stained S. cereus bacteria

Narrowing down the “Unknown Unknown”

(Belly Button)

• All G- bacteria• All bacteria shapes other

than coccus.• All bacteria not arranged in

clusters.

After Gram staining the culture of the Belly Button Bacteria and it’s colonies, we were able to eliminate all bacteria with the following attributes

Narrowing down the “Unknown Unknown”

(Leaf)

• All G- bacteria• All bacteria shapes other

than rods.• All bacteria not arranged at

random.

After Gram staining the culture of the Leaf Bacteria and it’s colonies, we were able to eliminate all bacteria with the following attributes

Which Bacteria Should Still Be Considered for the

“Unknown / Unknown”for the Leaf? 1. B. cereus• Info

2. B. subtilis• Info

2

0

2 2

0

1

2

3

Grow th Seen

0 = None1 = Slight

2 = Moderate3 - Abundant

PEA BA MSA -SALT

TSA

Type of Media Used

Gram + Bacteria Growth

S. epiderm idis

S. aureus

S. agalactiae

M. rosea

B. subtilis

B. cereus

Unknown/Unknown (BellyButton)

Known/Unknown (Leaf)

Which Bacteria Should Still Be Considered for the “Unknown / Belly

Button?1. S. epidermidis• Info Natural Flora found on Skin blah, blah, blah

2. S. aureus• Info

2

0

2 2

0

1

2

3

Grow th Seen

0 = None1 = Slight

2 = Moderate3 - Abundant

PEA BA MSA -SALT

TSA

Type of Media Used

Gram + Bacteria Growth

S. epiderm idis

S. aureus

S. agalactiae

M. rosea

B. subtilis

B. cereus

Unknown/Unknown (BellyButton)

Known/Unknown (Leaf)

A little something about our suspects for the Leaf

1. B. cereus• Info

2. B. subtilis• Info

A little something about our suspects for Belly Button

1. S. epidermis• Info

2. S. aureaus• Info

Growth TestsG- Bacteria on Variety of Media

3

1

2

0

0.5

1

1.5

2

2.5

3

3.5

PEA EMB SSA TSA

PEA = Phenylothanol BA = Blood AgerEMB = Eosin Methylene Blue (EMB)

SSA = Salmonella-Shigella AgarTSA = Trypticase Soy Agar

Growth0 = None1 = Slight

2 = Moderate3 - Abundant

P. vulgaris

K. pneum oniae

E. coli

S. m arcescens

E. aerogenes

P. aeruginosa

Belly Button (u/u)

Leaf (k/u)

Growth TestsG+ Bacteria on Variety of Media

0

1

PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS

S. epidermidis

S. aureus

S. agalactiae

M. rosea

B. subtil is

B. cereus

Unknown/Unknown (Bel ly Button)

Known/Unknown (Leaf)

Acid Key

0 = No

1 = Yes

Gas Key

0 = No Gas

1 = Yes, Gas

Media Used

Phenol Red Glucose (PRG)

Phenol Red Lactose (PRL)

Phenol Red Maltose (PRM)

Phenol Red Sucrose (PRS)

Phenol Red Arabinose (PRA)

Production of Carboxylic Acid & Gas Phenol Red Medias

Growth TestsG- Bacteria on Variety of Media

0

1

PRG - ACID PRG - GAS PRA - ACID PRA - GAS PRL - ACID PRL - GAS PRS- ACID PRS - GAS PRM - ACID PRM - GAS

P. vulgaris

K. pneumoniae

E. col i

S. marcescens

E. aerogenes

P. aeruginosa

Acid Key

0 = No

1 = Yes

Production of Carboxylic Acid & Gas Phenol Red Medias

Gas Key

0 = No Gas

1 = Yes, Gas

Media Used

Phenol Red Glucose (PRG)

Phenol Red Lactose (PRL)

Phenol Red Maltose (PRM)

Phenol Red Sucrose (PRS)

Phenol Red Arabinose (PRA)

Gram – Bacteria on a Variety of Media

2 2

1

2

0

1

2

3

Grow th Seen

0 = None1 = Slight

2 = Moderate3 - Abundant

PEA BA MSA -SALT

MSA -FERM

TSA

Type of Media

S. epidermidis

S. aureus

S. agalactiae

M . rosea

B. subtilis

B . cereus

Unknown/Unknown (Belly Button)

Known/Unknown (Leaf)

PARAGRAPHS

PARAGRAPHS