Transcript
Establishment of HIV-1 Establishment of HIV-1 resistance in T cells by resistance in T cells by zinc-finger nucleaseszinc-finger nucleases
By: Ryla BestBy: Ryla Best
BackgroundBackgroundHIV-1HIV-1T CellsT CellsCCR5CCR5ZFNsZFNs
Gene TherapiesGene TherapiesRNA inhibitorsRNA inhibitors
Antisense, ribozymes, RNAiAntisense, ribozymes, RNAiProtein inhibitorsProtein inhibitors
Zinc-finger, intrabodies/kinesZinc-finger, intrabodies/kines
Figure 1. (Perez, 2008)Figure 1. (Perez, 2008)
CCR5 MechanismCCR5 Mechanism
Figure 2. (Schoofs, 2008)Figure 2. (Schoofs, 2008)
Material and MethodsMaterial and MethodsZFNZFNGFP, GHOST-CCR5GFP, GHOST-CCR5NOG miceNOG mice
PCRPCRSurveyor nuclease assaySurveyor nuclease assayCytometryCytometry
NOG Mice MethodNOG Mice Method
Figure 3. (Perez, 2008)Figure 3. (Perez, 2008)
Results: CCR5 ExpressionResults: CCR5 Expression
Figure 4. (Perez, 2008)Figure 4. (Perez, 2008)
Results: CytometryResults: Cytometry
Figure 5. (Perez, 2008)Figure 5. (Perez, 2008)
Results: ZFNs SurvivalResults: ZFNs Survival
Figure 6. (Perez, 2008)Figure 6. (Perez, 2008)
Results: NOG MiceResults: NOG Mice
Figure 7. (Perez, 2008)Figure 7. (Perez, 2008)
ConclusionConclusionZFNs reduces CCR5 expressionZFNs reduces CCR5 expressionCan survive during HIV-1 infectionCan survive during HIV-1 infection Increase in T cell countIncrease in T cell countProtection vs. viremiaProtection vs. viremia
(Perez, 2008)(Perez, 2008)
Why is it important?Why is it important?HIV cureHIV cure ImmunotherapyImmunotherapyOther viral curesOther viral cures
Questions?Questions?
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