1. Choice of BufferThe pKa of the buffer should be within 0.5 unit ofthe desired pH (± 1 unit if you want to push it)Potential interactions with a column matrixAvoid UV-absorbing…
End of Semester Lectures 1) Coupled Assays 2) Buffers 3) Mechanism of AdhP 4) DNA Sequencing 5) AdhP Review Coupled Assays Anything that should be changed in the above assay?…