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Slide 1 Reverse Transcriptase: reversing the dogma Ariel (אָרּיּאּלּ) Grostern Seminar for the Guelph Symposium of CHEM*4550 Students Slide 2 Reversing the Dogma Standard…

Slide 1 DNA Technology Problem: A chromosome can be millions of base pairs long. How do you isolate and study a single gene? Scientists had to find out how to cut, paste,…

Slide 1 RNA Electrophoresis Slide 2 Broad and Long Term Objective To characterize the expression of ribulose 1-5 bisphosphate carboxylase oxygenase and chlorophyll AB binding…

Proteins: Sequence --> Structure --> Function Anfinsen Experiment: Denature ribonuclease (RNase) Remove denaturant Assay for RNase activity -- does the protein regain…

LECTURES 3/4. CONSTRUCTING and SCREENING cDNA LIBRARIES to ISOLATE NEW GENES ORIGINAL ARTICLES: CLONING BY COMPLEMENTATION: Lew, D, Dulic, V, and Reed SI. 1991. Isolation…