Slide 1Genome Sizes are Large human = 3 x 10 9 bp E. coli = 4 x 10 6 bp If 1 bp = 1 mm, then: human genome = 3000 km (1800 miles) E. coli genome = 4 km (2.5 miles) gene of…

PRINCIPLES OF DNA ISOLATION & PURIFICATION DNA can be isolated from any nucleated cell. DNA is a giant anion in solution. Sources of DNA include Blood Buccal cells Cultured…

Slide 1BIOTECHNOLOGY What can we do with DNA? Slide 2 Biotechnology Manipulation of biological organisms or their components for research and industrial purpose Usually manipulate…

Slide 1 Bio-Rad Science Ambassador Program Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors Slide 2 Bio-Rad Science Ambassador Program 2  Focuses…

Slide 1 Cell Structure: What we need to Extract: DNA EXTRACTION Slide 2 Extracting DNA at home 1/2 cup of split peas (100ml) 1/8 teaspoon table salt (less than 1ml) 1 cup…

Slide 1 Direct DNA transfer Introduce DNA into cells; assay expression immediately or select for permanently transformed cells. Techniques: 1.Chemical induction 2.Electroporation…

Slide 1 Extraction of Human DNA Slide 2 Experiment Goals Isolation of genomic DNA from human blood Analysis of isolated DNA using –Agarose gel electrophoresis –Spectrophotometry…

DNA Methodologies Sterilization Clean the workstation with alcohol and bleach. Autoclaving and ultraviolet light (UV radiation). Consumables and reagents. Equipment Pipettes-…

Extraction of Human DNA Experiment Goals Isolation of genomic DNA from human blood Analysis of isolated DNA using Agarose gel electrophoresis Spectrophotometry What is a…

S.KARTHIKUMAR,M.Sc.,M.Phil.,M.Tech.,(PhD) ASSISTANT PROFESSOR DEPARTMENT OF BIOTECHNOLOGY KAMARAJ COLLEGE OF ENGINEERING AND TECHNOLOGY VIRUDHUNAGAR-626001 TAMILNADU, INDIA…