Recombinant DNA Technology Recombinant DNA Technology PRESENTED BY: D.PRIYANKA M-PHARM DEPARTMENT OF PHARMACEUTICS UNDER GUIDENCE OF: Mrs.YASMIN BEGUM ASSOCIATE PROFFESSOR(Ph.D)…

1. Manipulating genes © Sir Ralph Riley 2. Ever since humans have been domesticating animals and raising crops they have been (unwittingly) manipulating genes By cross pollination…

Slide 1 pCambia Vectors Slide 2 The pCAMBIA vector backbone is derived from the pPZP vectors. the SphI site outside the right T-DNA Border, or the SacII site outside the…

Slide 1 Restriction Map: 1 PstI PstI + CcoIV 700030007000 4000 PstI CcoI 7Kbp 1 cut 2 cuts 3Kbp 4Kbp Slide 2 Restriction Map: 2 EcoRi EcoRI + BstEII 200100 800200900 700…

Slide 1 Restriction Map 1 Construct a plasmid restriction map of the following digest. Include cuts and fragment sizes. EcoRI EcoRI + HindIII 7,0006,00010,000 3,0003,000…

Slide 1 Slide 2 Plasmids  Bacterial DNA  Circular shape  Can be used to make proteins needed by humans Slide 3 Steps of Plasmid Use 1.Plasmid is isolated and used…

1.Recombinant DNA TechnologyBTEC33012. Recombinant DNA Technology Recombinant DNA technology procedures by which DNA from different species can be isolated, cut and spliced…

1. Recombinant DNATechnologyPRESENTED BY:D.PRIYANKAM-PHARMDEPARTMENT OF PHARMACEUTICSUNDER GUIDENCE OF:Mrs.YASMIN BEGUMASSOCIATE PROFFESSOR(Ph.D)MALLA REDDY COLLEGE OF PHARMACY…

Restriction Map: 1 7Kbp 1 cut 1 cut 2 cuts 3Kbp 4Kbp PstI PstI + CcoIV CcoIV 7000 3000 7000   4000   Restriction Map: 2 1,000 1 cut 1 cut 2 cuts 200 800 100 900 EcoRi EcoRI…