Slide 1Carry out PCR for 20, 25 and 30 cycles. Analyse the PCR fragments by agarose gel electrophoresis. Find out how the number of cycles affects the amount of DNA generated.…

Slide 1Useful hints and tips for cloning, PCR and site-directed mutagenesis Kirsten Jensen Division of Molecular Biosciences Slide 2 Overview Cloning (of PCR products) Polymerase…

APPLICATION OF PCR & VARIENTS OF PCR ISOLATION OF A GENE BY PCR If the Primers anneals anneal both the sides of the gene of interest No selection required THEN WHY CLONING…

Slide 1 Genetically Modified Golden Bean Mosaic Virus Resistant Beans Michael Xie 12/2/14 Slide 2 What's the big deal about beans? Common Bean (Phaseolus vulgaris L.)…

Slide 1 DNA Fingerprinting & Forensic Analysis Slide 2 Slide 3 Slide 4 How is DNA Typing Performed? Only one-tenth of 1% of DNA differs in each person; this variation…

Jahn Lab Dept. of Plant Breeding & Genetics, Cornell University Original Objectives Years 2-4 Generate a BAC library from Capsicum frutescens, a major source of disease…

Developmental Screen and Phase Changes Reverse Genetics PCR Genotyping Arabidopsis Experiments Phase Changes Phase Change Studies Genetic and molecular genetic approaches,…

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Supplementary figure 2 a. b. c. d. Supplementary figure 2. Generation of Unc5b mutant mice a. Schematic representation of UNC5B. Arrowhead indicates site of gene targeting…