-  molecular genetic tool to study protein-protein interactions -  if two proteins interact, then a reporter gene is transcriptionally activated -  Gal4 protein, transcriptional…

1. IDENTIFICATION OF INTERACTIONS BETWEEN LHX2 AND OTHER NEOCORTICAL PROTEINS Will Jackson Master’s Defense Talk March 17, 2015 2. Graded Expression: A gene being turned…

* CHAPTER 28 (Genes X) EUKARYOTIC TRANSCRIPTION REGULATION * Control of Gene Expression in Eukaryotes Eukaryotic gene expression is usually controlled at the level of initiation…

RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 650C for 3-15 min. 3. Cool on ice…

RT-PCR Obtain the internal region of cDNA 1. Mix crude RNA or poly(A) RNA, cDNA primer (one of the amplification primers in 7.) 2. Heat at 650C for 3-15 min. 3. Cool on ice…