Antiretroviral Drugs Classification & Highly Active Anti-Retroviral Therapy (HAART) Antiretroviral therapy (ART) is treatment of people infected with human immunodeficiency…
1. Multi system genetic disorder. Multiple organ tumors in Brain, kidneys and skin.According to the National Institute of Neurological Disorders and Stroke (NINDS),Tuberous…
1. RECOMBINANT PROTEINEXPRESSION BY KARIUKI M. SAMUEL ENOS KAMANI 2. INTRODUCTION• RPE is a subcomponent of gene expressionwhich involves translation of the gene intopolypeptide…
TIPS How to pick clones for a PCR ? You need : A plate containing the PCR amplification mix Primers, DNA pol, etc. It exists on the market various ready-mix, where only the…
DNA damage and repair DNA repair Damaged DNA must be repaired If the damage is passed on to subsequent generations, then we use the evolutionary term - mutation. It must…
Slide 1Plasmids Isolation of Plasmid DNA from Bacteria Properties of a Cloning/Expression Vector (Plasmid) Uses of Expression Vectors Slide 2 PLASMID DNA ISOLATION FROM E.Coli…
Slide 1 Class 11 – 2 nd “next generation” seq. method Review other sequencing methods Sanger Ion torrent New method Begin to consider uses of DNA seq. Slide 2 Old method…
Slide 1 Repair of replication errors by the MisMatch Repair System: Marking newly synthesized DNA in E. coli * GATC normally methylated on the A CTAG * Newly synthesized…
Slide 1 DNA repair CMB Pp 209-215 Slide 2 DNA repair Damaged DNA must be repaired If the damage is passed on to subsequent generations, then we use the evolutionary term…