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Documents Plasmids, primers (and beyond!). Or how to make green mice….

Slide 1Plasmids, primers (and beyond!) Slide 2 Or how to make green mice…. Slide 3 Foreign DNA can be incorporated into a cloning vector (plasmid, phage, YAC or other)…

Documents Ampicillin resistant Native promoter Wild type enzyme 5’5’3’3’ HindIII Start Codon Stop...

phoA in pEK8 Ampicillin resistant Native promoter Wild type enzyme 5â 3â HindIII Start Codon Stop Codon BsteII phoA in pEK8 Potential New Plasmids pET29a 5â 3â HindIII…