Slide 1Enzyme Kinetics C483 Spring 2013 Slide 2 Questions 1. Enzymes that join two substrates and require energy of a nucleoside triphosphate (such as ATP) to do so are called…

1.M.Prasad Naidu MSc Medical Biochemistry, Ph.D,. 2. Based on the type of reaction enzymes can be classified into SIX MAJORTYPES Classification of enzymes 1. Oxidoreductases-…

Slide 1 Enzymes Large molecules made of various amino acids Act as catalysts to speed up reactions w/out being destroyed –Highly specific –Lowers energy of activation…

Slide 1 Enzyme Kinetics: Study the rate of enzyme catalyzed reactions. - Models for enzyme kinetics - Michaelis-Menten kinetics - Inhibition kinetics - Effect of pH and Temperature…

Slide 1 General Outline for Antibiotics Chemistry –MIP Chemistry –MIP Effect on microbes - MIP Effect on microbes - MIP Spectrum of coverage Spectrum of coverage Mechanism(s)…

INTRODUCTION EXPERIMENT 4 Kinetics of Enzyme Reaction Todayâs Conference Introduction Objectives Methodology Actual Results and Interpretations Comparison with Theoretic…

[Substrate] affects rate and it changes during reaction Can measure just initial rate, Vo, when [S]>>[E] E + S ES E + P k1 k-1 k2 k-2 Slow step Rate-limiting Enzyme…