ﺒﺴﻡ ﺍﷲ ﺍﻟﺭﺤﻣﻥ ﺍﻟﺭﺤﻳﻡ ﺒﮌﺍ 24-11-2008 Morphologic Features of Anaplasia, Lack of Differentiation Pleomorphism: (pleo = more) (Variation…

Slide 1 More Molecular Genetic Technologies (Chapters 8, 9, 10) 1.Polymerase Chain Reaction (PCR) 1.Standard PCR 2.Real-time quantitative PCR 2.DNA sequencing 1.Manual dideoxy/automated…

Introduction to Cancer Introduction to Cancer Definitions The word âcancerâ comes from the latin word for crab. The Greek word oncos means swelling and where we get the…

Slide 1 PHYSICAL MAPPING AND POSITIONAL CLONING 1 Linkage mapping â Flanking markers identified â 1cM, for example Probably ~ 1 MB or more in humans Need very many families…

Slide 1 PHYSICAL MAPPING AND POSITIONAL CLONING 1 Linkage mapping â Flanking markers identified â 1cM, for example Probably ~ 1 MB or more in humans Need very many families…

Sequencing DNA 1. Maxam & Gilbert's method (chemical cleavage) 2. Fred Sanger's method (dideoxy method) 3. AUTOMATED sequencing (dideoxy, using fluorescent…