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Why Cryogenic Grinding?
• Many analytical samples are too flexible or sensitive to be ground at ambient temperatures
• Samples are subject to degrading• Does not damage DNA or RNA
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The Basics
• Samples are chilled at Liquid Nitrogen (LN) temperatures and ground using a Magnetically-Driven Impactor– No mechanical linkages or bearings to fail
• Sealed Grinding Vials ensure sample integrity
• Touch screen display w/ stored procedures for rapid process replication and consistency
© SPEX SamplePrep, Inc. 2011
Who Can Benefit From a Freezer/Mill®?
• Forensic Labs – DNA identification• Medical Labs– genetic research, disease
prevention• Hospital Labs – For genetic screening• Agricultural Labs – For crop development• Agricultural Veterinary Labs – For livestock
breeding and health• Archealogy Labs – Ancient DNA extraction• Public Health and Environmental Monitoring Labs• QC Testing of Manufactured Goods• Electronics Companies – Grinding Electronic
Components
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The Freezer/Mill® Family
Sample Size
0.6 – 5 g 0.6 – 100 g 0.6 – 200 g
LN System
Manual Manual, Auto-Fill Option Auto-Fill Only
Price $ $$ $$$
© SPEX SamplePrep, Inc. 2011
6970EFM – Features & Benefits
• Two Models Available– Single Grinding Chamber (6970M)
• 1 Grinding Chamber + 1 Pre-Cooling Chamber
– Dual Grinding Chambers (6970D)
• Totally enclosed auto-fill system with no Liquid Nitrogen exposure
• Increased through-put– Up to 8 small vials or 2 large vials
(D model only)
© SPEX SamplePrep, Inc. 2011
6770 – Features & Benefits
• Cost efficient• Runs one Micro-vial or small vial per run• Perfect for smaller samples (5 grams or less)• Additional area for pre-cooling samples • Unit has small counter footprint
© SPEX SamplePrep, Inc. 2011
6870 – Features & Benefits
• Versatile large grinding chamber– Runs a variety of vials, ranging from micro vials
through the large, even up to 4 of the small vials– Allows for wide range of sample sizes
• Available with Auto-Fill, or without
• Additional area for pre-cooling samples
© SPEX SamplePrep, Inc. 2011
Freezer/Mill Accessories
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Freezer/MillVials & Vial Sets
Micro Small Mid-Size Large
0.1 – 0.5 mL 0.5 – 4.0 mL Up to 25 mL Up to 50 mL
0.1 – 0.5 g 0.5 – 4.0 g Up to 40 g Up to 100 g
Vial Sizes
Vial Materials• Standard Set
• Stainless Steel End Plugs & Impactor with Polycarbonate Center Cylinder• Polycarbonate
• Avoids metal contamination• Stainless Steel
• Best for Polymer applications• Cr-Free
• For RoHS/WEEE appliactions
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Freezer/MillAuto-Extractor
6814 Auto-Extractor™• Works with Small and Large
size vials• Increases throughput• Reduces operator effort
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Freezer/MillAccessory Packages
6870S
6870L
6870MIncludes:
• 6754 Small Extractor• 6755 Vial Rack• 6806 Multi-Vial
AdapterIncludes:
• 6884 Mid-Size Adapter for 6804 Large Extractor
• 6886 Mid-Size Grinding Vial Adapter
Includes:
• 6804 Large Extractor• 6805 Vial Rack
© SPEX SamplePrep, Inc. 2011
Freezer/MillLiquid Nitrogen Handling
• Cryogenic Gloves
Liquid Nitrogen• Portable Dewars & Pressurized Tanks
– Required for use with Auto-Fill units(6870 + Auto-Fill & 6970EFM)
Safety
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Samples, Application Notes & Resources
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Before & After…
Leather
Corn
BoneA Collection of our Before &
After Samples
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Application Notes
• Extensive library of Freezer/Mill® applications
Examples• SP005 – RNA Extraction from Cartilage
Tissue Using Cryogenic Grinding• SP006 – Preparation of Rice Husks for
the Analysis of Their Contents• SP007 – Grinding Polymers For
Qualitative and Quantitative Analysis
Also Available:
Featured Articles Library
© SPEX SamplePrep, Inc. 2011
Product Demonstration Videos
