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Page 1: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

Teaching Biotechnology through Real-World Projects: Species Identification through

DNA Analysis

HFCC’s Biotechnology ProgramPaul Root

Jolie Stepaniak

Page 2: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

What is it?• Species identification can be difficult.

Page 3: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

What is it?

Page 4: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

What is it?

Page 5: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

Modern genetics techniques can be used to identify species.

• All living (or once-living) things contain DNA.

• Different species have different DNA sequences.

• Polymerase chain reaction (PCR) can make millions of copies of specific DNA sequences, which can then be analyzed.

Page 6: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

From: www.nobelprize.org

Page 7: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

Bio 261: Nucleic AcidsFinal Project

• Each student:– received a swab of cells

from an unknown organism.– designed and executed an

experiment using PCR to identify the source species.

Page 8: Teaching Biotechnology through Real-World Projects:  Species Identification through DNA Analysis

Benefits to students:• Challenging and fun• Authentic laboratory experience; real-world

problem-solving• Opportunity to demonstrate marketable skills


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