Dispatch Date : 29-Jan-2018
Page 1
Syphilis serology
Distribution : 4217
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
Your Score Intended Result Your Report
Specimen 4189 Reagin positive Reagin positive 2 TP Agglutination positive TP Agglutination positive 2 FTA-Abs positive FTA-Abs positive 2 Immunoassay IgG/total Ab positive Immunoassay IgG/total Ab positive 2 Immunoassay IgM positive Immunoassay IgM positive 2 Immunoblot positive Immunoblot positive 2
Specimen 4190 Reagin negative Reagin negative Not scored TP Agglutination positive TP Agglutination positive 2 FTA-Abs positive FTA-Abs positive Not scored Immunoassay IgG/total Ab positive Immunoassay IgG/total Ab positive 2 Immunoassay IgM negative Immunoassay IgM negative 2 Immunoblot positive Immunoblot positive Not scored
Specimen 4191 Reagin negative Reagin negative 2 TP Agglutination negative TP Agglutination negative 2 FTA-Abs negative FTA-Abs negative 2 Immunoassay IgG/total Ab negative Immunoassay IgG/total Ab negative 2 Immunoassay IgM negative Immunoassay IgM negative 2 Immunoblot negative Immunoblot negative 2
Specimen 4192 Reagin negative Reagin negative 2 TP Agglutination negative TP Agglutination negative 2 FTA-Abs negative FTA-Abs negative 2 Immunoassay IgG/total Ab negative Immunoassay IgG/total Ab negative 2 Immunoassay IgM negative Immunoassay IgM negative 2 Immunoblot negative Immunoblot negative 2
Specimen 4193 Reagin negative Reagin negative Not scored TP Agglutination positive TP Agglutination positive 2 FTA-Abs positive FTA-Abs positive 2 Immunoassay IgG/total Ab positive Immunoassay IgG/total Ab positive 2 Immunoassay IgM negative Immunoassay IgM negative 2 Immunoblot positive Immunoblot positive 2
Specimen 4194 Reagin negative Reagin negative Not scored TP Agglutination positive TP Agglutination positive 2 FTA-Abs positive FTA-Abs positive 2 Immunoassay IgG/total Ab positive Immunoassay IgG/total Ab positive 2 Immunoassay IgM negative Immunoassay IgM negative 2 Immunoblot positive Immunoblot positive 2
Please note that from this distribution onwards the report will have an additional graph displaying confirmatory test results(Syphilis line blot). Comments: Please check last page (Page 19) for additional comments. Results for each marker by methods used by participants are presented on subsequent pages. Images of the Fujirebio INNO-LIA IgG line assay of the positive specimens can be viewed on: www.ukneqasmicro.org.uk, by logging onto the Results and Reports section and selecting the 'Dist' button for the scheme. Turn around time: The time taken to report your results was 0-days. This information is provided for your own use and does not form part of your performance assessment. Acknowledgements: We thank colleagues at PHE- Public Health Laboratories in Birmingham, Bristol, Manchester and Newcastle; and the Department of Microbiology, Sheffield for assistance with pre-distribution tests. Enquiries: Any queries about this distribution please contact Beatrix Kele at the e-mail address below. Pre-distribution test results are available should you experience a technical failure and wish to discuss the results. For repeat specimens please order using the web form or e-mail [email protected] stating your laboratory identification number, the distribution number and specimen numbers. Report authorised by: Dr. Sanjiv Rughooputh, Scheme Organiser.
of 15
Dispatch Date : 29-Jan-2018
Page 2
Syphilis serology
Distribution : 4217
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
Cumulative score information Total number of specimens sent to you for UK NEQAS for Syphilis serology over the last 2 distributions is 12 For these distributions specimen numbers 3927 3928 3929 3930 3931 3932 4189 4190 4191 4192 4193 4194 have been sent. Number of specimens reported too late for analysis (not scored) 0 Your cumulative score for the specimen/test combinations that you reported was 122 out of a possible total of 122. The mean score calculated from the reports returned by UK laboratories testing the specimen/test combinations you examined was 119.44 (with a standard error of 2.28). Performance rating Your performance rating for UK NEQAS for Syphilis serology (i.e. the number of standard errors by which your cumulative score lies above or below the mean) for UK laboratories is 1.12. A performance rating of more than 1.96 standard errors below the mean indicates possible poor performance. For multiple analyte schemes no penalty is incurred for non return of reports. However non reporting may be used as a measure of performance. Your performance rating may change if other participants' results are amended.
Per
form
ance
Rat
ing
Distribution
Your performance rating over the past 12 distributions Your current performance rating is 1.12
MICPERF2
3059 3164 3266 3370 3508 3588 3720 3804 3944 4028 4131 4217-4-3-2-101234
All
UK
Tot
al s
core
Distribution
Total score you achieved for each of the last 12 distributionsYour total score for this distribution is 62
MICPERF2X
3059 3164 3266 3370 3508 3588 3720 3804 3944 4028 4131 421720
30
40
50
60
70
80
of 15
Dispatch Date : 29-Jan-2018
Page 3
Syphilis serology
Distribution : 4217
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4189 Syphilis serology positive Reagin positive
UK (%) All (%)
35 (50.7) 56 (23.7)Biokit RPR Reditest3 (4.3) 4 (1.7)Newmarket0 (0.0) 5 (2.1)Sclavo3 (4.3) 7 (3.0)BioRad1 (1.4) 8 (3.4)Unspecified1 (1.4) 14 (5.9)Plasmatec1 (1.4) 18 (7.6)Omega
18 (26.1) 26 (11.0)Axis-Shield1 (1.4) 27 (11.4)Becton Dickinson2 (2.9) 36 (15.3)bioMerieux
Number of Reports0 12 24 36 48 60
Positive Equivocal Negative
Your Report : Reagin positive
Reagin equivocal
69
Reagin positive
2
Overall Results
236
-1
% Correct
00
99.2
------------------------------------------------------------UK
2340
Total
1Reagin negative
69
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4189 Syphilis serology positive TP Agglutination positive
UK (%) All (%)
48 (80.0) 124 (61.1)Fujirebio : Serodia0 (0.0) 3 (1.5)Far Diagnostics0 (0.0) 3 (1.5)Sclavo1 (1.7) 4 (2.0)Randox0 (0.0) 4 (2.0)Unspecified0 (0.0) 6 (3.0)Plasmatec4 (6.7) 7 (3.4)Newmarket0 (0.0) 8 (3.9)Axis-Shield0 (0.0) 8 (3.9)Omega6 (10.0) 12 (5.9)Biokit: Syphagen
Number of Reports0 30 60 90 120 150
Positive Equivocal Negative
Your Report : TP Agglutination positive
TP Ag. equivocal
60
TP Ag. positive
1
Overall Results
203
-1
% Correct
00
99.5
------------------------------------------------------------UK
2020
Total
1TP Ag. negative
60
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4189 Syphilis serology positive FTA-Abs IgG positive
UK (%) All (%)
1 (100) 3 (14.3)Zeus0 (0.0) 1 (4.8)VIRO-IMMUN 0 (0.0) 2 (9.5)Scimedx0 (0.0) 4 (19.0)Alphadia0 (0.0) 4 (19.0)Unspecified0 (0.0) 7 (33.3)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs positive
FTA equivocal
1
FTA positive
0
Overall Results
21
-1
% Correct
00
100
------------------------------------------------------------UK
210
Total
1FTA negative
1
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 4
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4189 Syphilis serology positive Immunoassay IgG/total Ab positive
UK (%) All (%)
15 (9.7) 17 (4.5)Biokit : Bioelisa36 (23.2) 57 (15.2)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell5 (3.2) 8 (2.1)Trinity1 (0.6) 8 (2.1)BioRad2 (1.3) 8 (2.1)Unspecified
14 (9.0) 27 (7.2)Siemens:ADVIA Centaur11 (7.1) 45 (12.0)DiaSorin : Liaison52 (33.5) 155 (41.2)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab positive
Immunoassay equivocal
139
Immunoassay positive
2
Overall Results
342
-1
% Correct
10
99.4
------------------------------------------------------------UK
3400
Total
1Immunoassay negative
138
All
99.3
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4189 Syphilis serology positive Immunoassay IgM positive
UK (%) All (%)
10 (35.7) 13 (17.3)Mikrogen recomWell0 (0.0) 2 (2.7)BioRad2 (7.1) 3 (4.0)Captia2 (7.1) 4 (5.3)Lab 210 (0.0) 4 (5.3)Virotech1 (3.6) 5 (6.7)Unspecified0 (0.0) 7 (9.3)Mikrogen recomLine1 (3.6) 7 (9.3)Euroimmun2 (7.1) 9 (12.0)Diesse
10 (35.7) 14 (18.7)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM positive
IgM equivocal
28
IgM positive
1
Overall Results
75
-1
% Correct
10
98.7
------------------------------------------------------------UK
740
Total
1IgM negative
27
All
96.4
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4189 Syphilis serology positive Immunoblot positive
UK (%) All (%)
9 (90.0) 27 (55.1)Fujirebio INNO-LIA1 (10.0) 1 (2.0)DiaSorin : Liaison0 (0.0) 2 (4.1)Virotech0 (0.0) 3 (6.1)Euroimmun0 (0.0) 5 (10.2)Viramed0 (0.0) 5 (10.2)Mikrogen recomLine0 (0.0) 6 (12.2)Unspecified
Number of Reports0 6 12 18 24 30
Positive Equivocal Negative
Your Report : Immunoblot positive
Immunoblot equivocal
10
Immunoblot positive
0
Overall Results
49
-1
% Correct
00
100
------------------------------------------------------------UK
490
Total
1Immunoblot negative
10
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 5
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4190 Syphilis serology positive Reagin negative
UK (%) All (%)
36 (52.2) 57 (24.1)Biokit RPR Reditest0 (0.0) 5 (2.1)Human Diagnostics0 (0.0) 5 (2.1)Sclavo2 (2.9) 6 (2.5)BioRad1 (1.4) 8 (3.4)Unspecified1 (1.4) 14 (5.9)Plasmatec1 (1.4) 18 (7.6)Omega
18 (26.1) 26 (11.0)Axis-Shield1 (1.4) 27 (11.4)Becton Dickinson2 (2.9) 36 (15.2)bioMerieux
Number of Reports0 12 24 36 48 60
Positive Equivocal Negative
Your Report : Reagin negative
Reagin equivocal
69
Reagin positive
220
Overall Results
237
NS
% Correct
670
92.8
------------------------------------------------------------UK
170
Total
NSReagin negative
2
All
97.1
------------------------------------------------------------
NS
Score
Your Score : Not scored
Specimen : 4190 Syphilis serology positive TP Agglutination positive
UK (%) All (%)
48 (81.4) 124 (61.7)Fujirebio : Serodia0 (0.0) 3 (1.5)Far Diagnostics0 (0.0) 3 (1.5)Sclavo0 (0.0) 3 (1.5)Unspecified1 (1.7) 4 (2.0)Randox0 (0.0) 6 (3.0)Plasmatec4 (6.8) 7 (3.5)Newmarket0 (0.0) 8 (4.0)Axis-Shield0 (0.0) 8 (4.0)Omega6 (10.2) 12 (6.0)Biokit: Syphagen
Number of Reports0 30 60 90 120 150
Positive Equivocal Negative
Your Report : TP Agglutination positive
TP Ag. equivocal
59
TP Ag. positive
17
Overall Results
201
-1
% Correct
03
90.0
------------------------------------------------------------UK
1810
Total
1TP Ag. negative
59
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4190 Syphilis serology positive FTA-Abs IgG positive
UK (%) All (%)
1 (100) 3 (13.6)Zeus0 (0.0) 1 (4.5)VIRO-IMMUN 0 (0.0) 2 (9.1)Scimedx0 (0.0) 4 (18.2)Alphadia0 (0.0) 5 (22.7)Unspecified0 (0.0) 7 (31.8)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs positive
FTA equivocal
2
FTA positive
4
Overall Results
22
NS
% Correct
12
72.7
------------------------------------------------------------UK
160
Total
NSFTA negative
1
All
50.0
------------------------------------------------------------
NS
Score
Your Score : Not scored
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 6
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4190 Syphilis serology positive Immunoassay IgG/total Ab positive
UK (%) All (%)
15 (9.7) 17 (4.5)Biokit : Bioelisa36 (23.4) 57 (15.2)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell0 (0.0) 7 (1.9)BioRad2 (1.3) 7 (1.9)Unspecified5 (3.2) 8 (2.1)Trinity
14 (9.1) 27 (7.2)Siemens:ADVIA Centaur11 (7.1) 45 (12.0)DiaSorin : Liaison52 (33.8) 155 (41.4)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab positive
Immunoassay equivocal
137
Immunoassay positive
2
Overall Results
340
-1
% Correct
02
98.8
------------------------------------------------------------UK
3360
Total
1Immunoassay negative
137
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4190 Syphilis serology positive Immunoassay IgM negative
UK (%) All (%)
9 (32.1) 12 (16.0)Mikrogen recomWell0 (0.0) 2 (2.7)BioRad2 (7.1) 3 (4.0)Captia2 (7.1) 4 (5.3)Lab 210 (0.0) 4 (5.3)Virotech1 (3.6) 5 (6.7)Unspecified0 (0.0) 7 (9.3)Mikrogen recomLine1 (3.6) 7 (9.3)Euroimmun2 (7.1) 9 (12.0)Diesse
11 (39.3) 15 (20.0)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM negative
IgM equivocal
28
IgM positive
73
Overall Results
75
2
% Correct
260
97.3
------------------------------------------------------------UK
20
Total
0IgM negative
2
All
92.9
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4190 Syphilis serology positive Immunoblot positive
UK (%) All (%)
9 (90.0) 27 (56.3)Fujirebio INNO-LIA1 (10.0) 1 (2.1)DiaSorin : Liaison0 (0.0) 2 (4.2)Virotech0 (0.0) 2 (4.2)Euroimmun0 (0.0) 4 (8.3)Viramed0 (0.0) 6 (12.5)Mikrogen recomLine0 (0.0) 6 (12.5)Unspecified
Number of Reports0 6 12 18 24 30
Positive Equivocal Negative
Your Report : Immunoblot positive
Immunoblot equivocal
10
Immunoblot positive
4
Overall Results
48
NS
% Correct
06
79.2
------------------------------------------------------------UK
380
Total
NSImmunoblot negative
10
All
100
------------------------------------------------------------
NS
Score
Your Score : Not scored
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 7
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4191 Syphilis serology negative Reagin negative
UK (%) All (%)
19 (48.7) 34 (19.3)Biokit RPR Reditest0 (0.0) 3 (1.7)Immucor/Immunotrep0 (0.0) 3 (1.7)BioRad0 (0.0) 5 (2.8)Sclavo1 (2.6) 7 (4.0)Unspecified1 (2.6) 14 (8.0)Plasmatec0 (0.0) 16 (9.1)Omega
13 (33.3) 20 (11.4)Axis-Shield1 (2.6) 22 (12.5)Becton Dickinson0 (0.0) 27 (15.3)bioMerieux
Number of Reports0 8 16 24 32 40
Positive Equivocal Negative
Your Report : Reagin negative
Reagin equivocal
39
Reagin positive
176
Overall Results
176
2
% Correct
390
100
------------------------------------------------------------UK
00
Total
1Reagin negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4191 Syphilis serology negative TP Agglutination negative
UK (%) All (%)
27 (77.1) 83 (55.3)Fujirebio : Serodia0 (0.0) 2 (1.3)Abbott : Architect0 (0.0) 3 (2.0)Fortress0 (0.0) 3 (2.0)Sclavo0 (0.0) 3 (2.0)Randox0 (0.0) 6 (4.0)Plasmatec3 (8.6) 6 (4.0)Newmarket0 (0.0) 8 (5.3)Axis-Shield0 (0.0) 8 (5.3)Omega5 (14.3) 11 (7.3)Biokit: Syphagen
Number of Reports0 20 40 60 80 100
Positive Equivocal Negative
Your Report : TP Agglutination negative
TP Ag. equivocal
35
TP Ag. positive
150
Overall Results
150
2
% Correct
350
100
------------------------------------------------------------UK
00
Total
1TP Ag. negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4191 Syphilis serology negative FTA-Abs IgG negative
UK (%) All (%)
1 (100) 3 (15.8)Zeus0 (0.0) 2 (10.5)Scimedx0 (0.0) 4 (21.1)Alphadia0 (0.0) 4 (21.1)Unspecified0 (0.0) 6 (31.6)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs negative
FTA equivocal
1
FTA positive
19
Overall Results
19
2
% Correct
10
100
------------------------------------------------------------UK
00
Total
0FTA negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 8
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4191 Syphilis serology negative Immunoassay IgG/total Ab negative
UK (%) All (%)
15 (9.9) 17 (4.6)Biokit : Bioelisa35 (23.0) 56 (15.1)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell0 (0.0) 7 (1.9)BioRad5 (3.3) 8 (2.2)Trinity2 (1.3) 8 (2.2)Unspecified
14 (9.2) 26 (7.0)Siemens:ADVIA Centaur11 (7.2) 45 (12.1)DiaSorin : Liaison52 (34.2) 155 (41.7)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab negative
Immunoassay equivocal
138
Immunoassay positive
339
Overall Results
340
2
% Correct
1380
99.7
------------------------------------------------------------UK
10
Total
1Immunoassay negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4191 Syphilis serology negative Immunoassay IgM negative
UK (%) All (%)
5 (31.3) 6 (10.5)Mikrogen recomWell2 (12.5) 2 (3.5)Captia0 (0.0) 2 (3.5)BioRad1 (6.3) 3 (5.3)Lab 210 (0.0) 3 (5.3)Virotech1 (6.3) 4 (7.0)Unspecified0 (0.0) 6 (10.5)Mikrogen recomLine0 (0.0) 6 (10.5)Euroimmun0 (0.0) 7 (12.3)Diesse7 (43.8) 11 (19.3)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM negative
IgM equivocal
16
IgM positive
57
Overall Results
57
2
% Correct
160
100
------------------------------------------------------------UK
00
Total
0IgM negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4191 Syphilis serology negative Immunoblot negative
UK (%) All (%)
4 (80.0) 10 (45.5)Fujirebio INNO-LIA0 (0.0) 1 (4.5)Viramed1 (20.0) 1 (4.5)DiaSorin : Liaison0 (0.0) 2 (9.1)Virotech0 (0.0) 2 (9.1)Euroimmun0 (0.0) 3 (13.6)Mikrogen recomLine0 (0.0) 3 (13.6)Unspecified
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoblot negative
Immunoblot equivocal
5
Immunoblot positive
22
Overall Results
22
2
% Correct
50
100
------------------------------------------------------------UK
00
Total
1Immunoblot negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 9
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4192 Syphilis serology negative Reagin negative
UK (%) All (%)
20 (48.8) 35 (19.7)Biokit RPR Reditest0 (0.0) 3 (1.7)Immucor/Immunotrep1 (2.4) 4 (2.2)BioRad0 (0.0) 5 (2.8)Sclavo1 (2.4) 7 (3.9)Unspecified1 (2.4) 14 (7.9)Plasmatec0 (0.0) 16 (9.0)Omega
13 (31.7) 20 (11.2)Axis-Shield1 (2.4) 22 (12.4)Becton Dickinson0 (0.0) 27 (15.2)bioMerieux
Number of Reports0 8 16 24 32 40
Positive Equivocal Negative
Your Report : Reagin negative
Reagin equivocal
41
Reagin positive
178
Overall Results
178
2
% Correct
410
100
------------------------------------------------------------UK
00
Total
1Reagin negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4192 Syphilis serology negative TP Agglutination negative
UK (%) All (%)
28 (75.7) 84 (55.3)Fujirebio : Serodia0 (0.0) 2 (1.3)Abbott : Architect0 (0.0) 3 (2.0)Fortress0 (0.0) 3 (2.0)Sclavo0 (0.0) 3 (2.0)Randox0 (0.0) 6 (3.9)Plasmatec3 (8.1) 6 (3.9)Newmarket0 (0.0) 8 (5.3)Axis-Shield0 (0.0) 8 (5.3)Omega5 (13.5) 11 (7.2)Biokit: Syphagen
Number of Reports0 20 40 60 80 100
Positive Equivocal Negative
Your Report : TP Agglutination negative
TP Ag. equivocal
37
TP Ag. positive
151
Overall Results
152
2
% Correct
360
99.3
------------------------------------------------------------UK
10
Total
1TP Ag. negative
1
All
97.3
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4192 Syphilis serology negative FTA-Abs IgG negative
UK (%) All (%)
1 (100) 3 (15.8)Zeus0 (0.0) 2 (10.5)Scimedx0 (0.0) 4 (21.1)Alphadia0 (0.0) 4 (21.1)Unspecified0 (0.0) 6 (31.6)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs negative
FTA equivocal
1
FTA positive
18
Overall Results
19
2
% Correct
10
94.7
------------------------------------------------------------UK
10
Total
0FTA negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 10
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4192 Syphilis serology negative Immunoassay IgG/total Ab negative
UK (%) All (%)
15 (9.8) 17 (4.6)Biokit : Bioelisa35 (22.9) 56 (15.0)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell5 (3.3) 8 (2.1)Trinity1 (0.7) 8 (2.1)BioRad2 (1.3) 8 (2.1)Unspecified
14 (9.2) 27 (7.2)Siemens:ADVIA Centaur11 (7.2) 44 (11.8)DiaSorin : Liaison52 (34.0) 155 (41.6)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab negative
Immunoassay equivocal
139
Immunoassay positive
339
Overall Results
342
2
% Correct
1380
99.1
------------------------------------------------------------UK
30
Total
1Immunoassay negative
1
All
99.3
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4192 Syphilis serology negative Immunoassay IgM negative
UK (%) All (%)
5 (31.3) 6 (10.5)Mikrogen recomWell2 (12.5) 2 (3.5)Captia0 (0.0) 2 (3.5)BioRad1 (6.3) 3 (5.3)Lab 210 (0.0) 3 (5.3)Virotech1 (6.3) 4 (7.0)Unspecified0 (0.0) 6 (10.5)Mikrogen recomLine0 (0.0) 6 (10.5)Euroimmun0 (0.0) 7 (12.3)Diesse7 (43.8) 11 (19.3)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM negative
IgM equivocal
16
IgM positive
57
Overall Results
57
2
% Correct
160
100
------------------------------------------------------------UK
00
Total
0IgM negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4192 Syphilis serology negative Immunoblot negative
UK (%) All (%)
4 (80.0) 10 (50.0)Fujirebio INNO-LIA0 (0.0) 1 (5.0)Viramed0 (0.0) 1 (5.0)Euroimmun1 (20.0) 1 (5.0)DiaSorin : Liaison0 (0.0) 2 (10.0)Virotech0 (0.0) 2 (10.0)Unspecified0 (0.0) 3 (15.0)Mikrogen recomLine
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoblot negative
Immunoblot equivocal
5
Immunoblot positive
20
Overall Results
20
2
% Correct
50
100
------------------------------------------------------------UK
00
Total
1Immunoblot negative
0
All
100
------------------------------------------------------------
-1
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 11
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4193 Syphilis serology positive Reagin negative
UK (%) All (%)
35 (50.7) 56 (23.6)Biokit RPR Reditest0 (0.0) 5 (2.1)Human Diagnostics0 (0.0) 5 (2.1)Sclavo3 (4.3) 7 (3.0)BioRad1 (1.4) 8 (3.4)Unspecified1 (1.4) 14 (5.9)Plasmatec1 (1.4) 18 (7.6)Omega
18 (26.1) 26 (11.0)Axis-Shield1 (1.4) 27 (11.4)Becton Dickinson2 (2.9) 36 (15.2)bioMerieux
Number of Reports0 12 24 36 48 60
Positive Equivocal Negative
Your Report : Reagin negative
Reagin equivocal
69
Reagin positive
212
Overall Results
237
NS
% Correct
632
89.5
------------------------------------------------------------UK
230
Total
NSReagin negative
6
All
91.3
------------------------------------------------------------
NS
Score
Your Score : Not scored
Specimen : 4193 Syphilis serology positive TP Agglutination positive
UK (%) All (%)
47 (79.7) 122 (61.0)Fujirebio : Serodia0 (0.0) 3 (1.5)Far Diagnostics0 (0.0) 3 (1.5)Sclavo0 (0.0) 3 (1.5)Unspecified1 (1.7) 4 (2.0)Randox0 (0.0) 6 (3.0)Plasmatec4 (6.8) 7 (3.5)Newmarket0 (0.0) 8 (4.0)Axis-Shield0 (0.0) 8 (4.0)Omega6 (10.2) 12 (6.0)Biokit: Syphagen
Number of Reports0 30 60 90 120 150
Positive Equivocal Negative
Your Report : TP Agglutination positive
TP Ag. equivocal
59
TP Ag. positive
5
Overall Results
200
-1
% Correct
01
97.0
------------------------------------------------------------UK
1940
Total
1TP Ag. negative
59
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4193 Syphilis serology positive FTA-Abs IgG positive
UK (%) All (%)
1 (100) 3 (14.3)Zeus0 (0.0) 1 (4.8)VIRO-IMMUN 0 (0.0) 2 (9.5)Scimedx0 (0.0) 4 (19.0)Alphadia0 (0.0) 4 (19.0)Unspecified0 (0.0) 7 (33.3)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs positive
FTA equivocal
1
FTA positive
1
Overall Results
21
-1
% Correct
00
95.2
------------------------------------------------------------UK
200
Total
1FTA negative
1
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 12
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4193 Syphilis serology positive Immunoassay IgG/total Ab positive
UK (%) All (%)
15 (9.7) 17 (4.5)Biokit : Bioelisa36 (23.2) 57 (15.1)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell5 (3.2) 8 (2.1)Trinity1 (0.6) 8 (2.1)BioRad2 (1.3) 9 (2.4)Unspecified
14 (9.0) 27 (7.2)Siemens:ADVIA Centaur11 (7.1) 45 (11.9)DiaSorin : Liaison52 (33.5) 155 (41.1)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab positive
Immunoassay equivocal
139
Immunoassay positive
3
Overall Results
342
-1
% Correct
10
99.1
------------------------------------------------------------UK
3390
Total
1Immunoassay negative
138
All
99.3
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4193 Syphilis serology positive Immunoassay IgM negative
UK (%) All (%)
9 (32.1) 12 (16.0)Mikrogen recomWell0 (0.0) 2 (2.7)BioRad2 (7.1) 3 (4.0)Captia2 (7.1) 4 (5.3)Lab 210 (0.0) 4 (5.3)Virotech1 (3.6) 6 (8.0)Euroimmun1 (3.6) 6 (8.0)Unspecified0 (0.0) 7 (9.3)Mikrogen recomLine2 (7.1) 9 (12.0)Diesse
11 (39.3) 15 (20.0)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM negative
IgM equivocal
28
IgM positive
69
Overall Results
75
2
% Correct
262
92.0
------------------------------------------------------------UK
41
Total
0IgM negative
1
All
92.9
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4193 Syphilis serology positive Immunoblot positive
UK (%) All (%)
9 (90.0) 26 (53.1)Fujirebio INNO-LIA1 (10.0) 1 (2.0)DiaSorin : Liaison0 (0.0) 2 (4.1)Virotech0 (0.0) 3 (6.1)Euroimmun0 (0.0) 5 (10.2)Viramed0 (0.0) 6 (12.2)Mikrogen recomLine0 (0.0) 6 (12.2)Unspecified
Number of Reports0 6 12 18 24 30
Positive Equivocal Negative
Your Report : Immunoblot positive
Immunoblot equivocal
10
Immunoblot positive
3
Overall Results
49
-1
% Correct
03
87.8
------------------------------------------------------------UK
430
Total
1Immunoblot negative
10
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 13
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4194 Syphilis serology positive Reagin negative
UK (%) All (%)
36 (51.4) 57 (23.9)Biokit RPR Reditest0 (0.0) 5 (2.1)Human Diagnostics0 (0.0) 5 (2.1)Sclavo3 (4.3) 7 (2.9)BioRad1 (1.4) 8 (3.4)Unspecified1 (1.4) 14 (5.9)Plasmatec1 (1.4) 18 (7.6)Omega
18 (25.7) 26 (10.9)Axis-Shield1 (1.4) 27 (11.3)Becton Dickinson2 (2.9) 36 (15.1)bioMerieux
Number of Reports0 12 24 36 48 60
Positive Equivocal Negative
Your Report : Reagin negative
Reagin equivocal
70
Reagin positive
213
Overall Results
238
NS
% Correct
661
89.5
------------------------------------------------------------UK
240
Total
NSReagin negative
4
All
94.3
------------------------------------------------------------
NS
Score
Your Score : Not scored
Specimen : 4194 Syphilis serology positive TP Agglutination positive
UK (%) All (%)
48 (80.0) 123 (61.2)Fujirebio : Serodia0 (0.0) 3 (1.5)Far Diagnostics0 (0.0) 3 (1.5)Sclavo0 (0.0) 3 (1.5)Unspecified1 (1.7) 4 (2.0)Randox0 (0.0) 6 (3.0)Plasmatec4 (6.7) 7 (3.5)Newmarket0 (0.0) 8 (4.0)Axis-Shield0 (0.0) 8 (4.0)Omega6 (10.0) 12 (6.0)Biokit: Syphagen
Number of Reports0 30 60 90 120 150
Positive Equivocal Negative
Your Report : TP Agglutination positive
TP Ag. equivocal
60
TP Ag. positive
2
Overall Results
201
-1
% Correct
00
99.0
------------------------------------------------------------UK
1990
Total
1TP Ag. negative
60
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4194 Syphilis serology positive FTA-Abs IgG positive
UK (%) All (%)
1 (100) 3 (14.3)Zeus0 (0.0) 1 (4.8)VIRO-IMMUN 0 (0.0) 2 (9.5)Scimedx0 (0.0) 4 (19.0)Alphadia0 (0.0) 4 (19.0)Unspecified0 (0.0) 7 (33.3)Euroimmun
Number of Reports0 2 4 6 8 10
Positive Equivocal Negative
Your Report : FTA-Abs positive
FTA equivocal
1
FTA positive
1
Overall Results
21
-1
% Correct
00
95.2
------------------------------------------------------------UK
200
Total
1FTA negative
1
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Dispatch Date : 29-Jan-2018
Page 14
Syphilis serology
Distribution : 4217
Laboratory :
Six sera (plasma converted by thrombinisation) were issued with requests to report on antibody reactivity indicating the presence of Treponema pallidum (TP). Specimens 4193 and 4194 were pool of two donations. Specimens 4189 and 4190 were single donations of syphilis positive material and specimen 4191 and 4192 were single donations of negative specimens.
Specimen : 4194 Syphilis serology positive Immunoassay IgG/total Ab positive
UK (%) All (%)
15 (9.7) 17 (4.5)Biokit : Bioelisa36 (23.2) 57 (15.2)Roche
2 (1.3) 4 (1.1)Murex : ICE0 (0.0) 5 (1.3)Vircell5 (3.2) 8 (2.1)Trinity1 (0.6) 8 (2.1)BioRad2 (1.3) 8 (2.1)Unspecified
14 (9.0) 27 (7.2)Siemens:ADVIA Centaur11 (7.1) 44 (11.7)DiaSorin : Liaison52 (33.5) 155 (41.3)Abbott : Architect
Number of Reports0 40 80 120 160 200
Positive Equivocal Negative
Your Report : Immunoassay IgG/total Ab positive
Immunoassay equivocal
139
Immunoassay positive
1
Overall Results
342
-1
% Correct
00
99.7
------------------------------------------------------------UK
3410
Total
1Immunoassay negative
139
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Specimen : 4194 Syphilis serology positive Immunoassay IgM negative
UK (%) All (%)
9 (32.1) 12 (16.0)Mikrogen recomWell0 (0.0) 2 (2.7)BioRad2 (7.1) 3 (4.0)Captia2 (7.1) 4 (5.3)Lab 210 (0.0) 4 (5.3)Virotech1 (3.6) 5 (6.7)Unspecified0 (0.0) 7 (9.3)Mikrogen recomLine1 (3.6) 7 (9.3)Euroimmun2 (7.1) 9 (12.0)Diesse
11 (39.3) 15 (20.0)Trinity
Number of Reports0 4 8 12 16 20
Positive Equivocal Negative
Your Report : Immunoassay IgM negative
IgM equivocal
28
IgM positive
67
Overall Results
75
2
% Correct
273
89.3
------------------------------------------------------------UK
50
Total
0IgM negative
1
All
96.4
------------------------------------------------------------
-1
Score
Your Score : 2
Specimen : 4194 Syphilis serology positive Immunoblot positive
UK (%) All (%)
9 (90.0) 26 (54.2)Fujirebio INNO-LIA1 (10.0) 1 (2.1)DiaSorin : Liaison0 (0.0) 2 (4.2)Virotech0 (0.0) 3 (6.3)Euroimmun0 (0.0) 5 (10.4)Viramed0 (0.0) 5 (10.4)Mikrogen recomLine0 (0.0) 6 (12.5)Unspecified
Number of Reports0 6 12 18 24 30
Positive Equivocal Negative
Your Report : Immunoblot positive
Immunoblot equivocal
10
Immunoblot positive
3
Overall Results
48
-1
% Correct
00
93.8
------------------------------------------------------------UK
450
Total
1Immunoblot negative
10
All
100
------------------------------------------------------------
2
Score
Your Score : 2
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15
Comments on distribution 4217
Comments
Overall performance was very good with correct reports for scored specimens of 99.7% for reagin, 97.5% for TP Agglutination, 97% for FTA, 99.3% for Immunoassay IgG/total, 96.3% for Immunoblot and 96.2% for Immunoassay IgM.
Specimens 4190, 4193 and 4194 Reagin, not scored Experience has shown that treponemal antibody positive specimens with reagin titres of less
than 1:4 may be reported as negative by a significant number of participants, due to variation in test sensitivity, local reporting practice, and to the normal statistical variation that is likely to occur. Only those specimens having reagin titres of 1:4 or more during pre-distribution
testing will be included in scoring (Guidance from the National Quality Assurance Advisory Panel).
TP agglutination titres:
TP agglutination titres were reported as follows: Specimen 4189: median titre 1:2560 (range 1:5 to 1:20480), 167 labs
Specimen 4190: median titre 1:160 (range 1:1 to 1:1280), 155 labs Specimen 4193: median titre 1:160 (range 1:1 to 1:2560), 162 labs Specimen 4194: median titre 1:640 (range 1:2 to 1:10240), 165 labs
Specimens 4191 and 4192 were negative for treponemal antibodies.
Page 15
Syphilis serology
Distribution : 4217
Laboratory :
Operated by Public Health England NIS - Specialist Microbiology Services 133-155 Waterloo Road Wellington House London SE1 8UG
© Copyright. The data in UK NEQAS reports are confidential. Participants must consult the scheme organiser before quoting data from the scheme. UK NEQAS for Microbiology PO Box 63003 London NW9 1GH Published at 8:26 on Thursday 12 July 2018
of 15