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Observing Behavior at the Nanoscale using an Optical Microscope
Eann PattersonComposite Vehicle Research Center
Michigan State University
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Olympus IX70 Inverted Tissue Culture Microscope
high resolution (1324x1024) 16-bit monochrome cooled
CCD camera
X60 objective (0.7 NA)
550nm with 45nm bandwidth
Diaphragm aperture
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10m dia (10,000nm) sphere ≡1/8 diameter of human hair
wavelength of light (550nm)
Diffraction Limit(220nm)
100nm diasilica sphere ≡1/1000 human hair
Resolving Nano-particles
[Patterson & Whelan, Nanotechnology, 19(10)2008]
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Compositeimages
100nm silica particle in 3D
[Patterson & Whelan, Nanotechnology, 19(10)2008]
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Brownian motion of 300nm particles
Silica particles in ethanol: field of view 140x100mm
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20000 nm
Image of fibroblast cell in optical microscope using phase contrastwith 100nm diameter nanoparticles
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20000 nm
Image of fibroblast cell in optical microscope in nanoscope modewith 100nm diameter nanoparticles
at t=0
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20000 nm
Image of fibroblast cell in optical microscope nanoscope modewith 100nm diameter nanoparticles
at t=100ms
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20000 nm
5000nm/100ms ≡ 50 m/s 2 m/hr
Image of fibroblast cell in optical microscope nanoscope modewith 100nm diameter nanoparticles
at t=100ms
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Acknowledgements
Professor Maurice P. Whelan
“Nothing tends so much to the advancement of knowledge as the application of a new instrument.”Elements of Chemical Philosophy (1812), in J. Davy (ed.), The Collected Works of Sir Humphry Davy(1839-40), Vol. 4, 37.
