http://lawrencekok.blogspot.com
Prepared by Lawrence Kok
Video Tutorial on Chromatography, Paper, Thin Layer, Column Chromatography, HPLC and GLC.
Click here to view TLC and Column ChromatographySeparation using TLC TLC techniques step by step
Column Chromatography Column chromatography separation
Click here to view HPLC and Gas Liquid Chromatography
HPLC works Gas Liquid Chromatography works
Chromatography
Partition Chromatography vs Adsorption Chromatography
Partition Chromatography Adsorption Chromatography
Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html
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Pic taken from:http://www.liv.ac.uk/~agmclen/Medpracs/practical_4/theory_4.html
Mobile phase - Liquid Mobile phase - LiquidStationary phase - Liquid Stationary phase - Solid
Partition Chromatography Adsorption Chromatography
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Distribution of X, Y and Zbetween two liquid phases
Adsorption of X, Y and Z onto SOLID phase
Type of chromatography
Type of chromatography
Type of chromatography
Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)
Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)
Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)
Type of chromatography
Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)
Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)
Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)
Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent
Type of chromatography
Stationary phase – Cellulose paper absorb layer of aqueous water (liquid phase)
Stationary phase – Solid silica SiO2 or alumina AI2O3 (solid phase)
Stationary phase –Solid silica SiO2 or alumina AI2O3 packed in column(solid phase)
Mobile phase is liquid solvent Mobile phase is liquid solvent Mobile phase is liquid solvent
Solvent moves by capillary actionPartition/Distribution of solutebetween 2 liquid phase
Adsorption of solute onto Stationary solid phase
Adsorption of solute on Stationary phase- strongly adsorp – travel slowly (eluted last)- weakly adsorp – travel fast (eluted first)
Separated components measured using Rf values
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Click here to viewSeparation using TLC TLC techniques step by step
Column Chromatography Column chromatography separation
Type of chromatography
Type of chromatography
We also have HPLC and GLC
Pic from:http://www.comsol.com/stories/waters_corp_hplc_systems/full/ Pic from:http://www.sfu.ca/bisc/bisc-429/GLC.html
High Performance Liquid Chromatography Gas Liquid Chromatography
High Performance Liquid Chromatography Gas Liquid Chromatography
Click here to view
HPLC works Gas Liquid Chromatography works
Summary
Paper Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid)
Thin Layer Chromatography - Adsorption Chromatography- Mobile phase (Liquid)- Stationary phase (Solid)
Column Chromatography - Adsorption Chromatography - Mobile phase (Liquid) - Stationary phase (Solid)
High Performance Liquid Chromatography - Partition Chromatography - Mobile phase (Liquid) - Stationary phase (Liquid)
Gas Liquid Chromatography – Partition Chromatography - Mobile phase (Gas) - Stationary phase (Liquid)
Acknowledgements
Thanks to source of pictures and video used in this presentation
Thanks to Creative Commons for excellent contribution on licenseshttp://creativecommons.org/licenses/
Prepared by Lawrence Kok
Check out more video tutorials from my site and hope you enjoy this tutorialhttp://lawrencekok.blogspot.com
