Versette Onboard Programming
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Versette Onboard Programming GUI Interface
• Applications: Academia, pharma and biotech including drug discovery
(including CROs), cell-based, genomics and proteomics. Ideal for life
science researchers in low- to high-throughput laboratories currently
performing hand-held pipetting tasks who want to move to automation
or researchers putting together integrated systems that require liquid
handling.
• Intuitive and easy to use
• Full color touch panel display
• Graphically based and inviting to look at
• Most options are preset and allow toggling between options
• Guides the user through program creation
• Testing capability through preview screen
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• Steps• Serial Dilution Column-wise Across 96 Well Microplate
• Remove Excess Volume from Last Column of Microplate
• Dispose of Excess Volume from Last Column
IC50 Serial Dilution/ 8-Channel Pipetting Procedure
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 1: Select New Program
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 2: Enter Program Name
Select Next
IC50
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 3: Select Pipetting Head Type from RFID Scanned List Using Arrows on Right
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 4: Select Pipetting Head Substation Pick-up and Drop-off Information
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 6: Select Select Tips
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 8: Select Clip Tip Pick up Stage, Rack Location, and Waste Location
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 9: Preview
Select Insert to add a step to the Head and Tip Configuration
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 10: Choose Serial Dilute from the first of two selection menus to add a Serial Dilute Procedure to the program
(Select More to access the second set)
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 11: Select Stage used for Serial Dilution; Stage will Turn Blue once a selection is made
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 11: Select Labware on Stage 3
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 12: The Serial Dilute Procedure has two menus of parameters to configure. When adjusting these parameters,
differences will appear to the right of the pressed button or a sub-menu will pop up (Start and EndRow/Colum, Volume, Well Map,
and Mix Between Volume all have pop-up menus that appear when pressed).
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 13: These are pop-up menus that appear when Well Map and Mix Between Volume is pressed.
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 14: Preview
Select Save
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 15: Confirm and modify (if necessary) the program name
Select Next to Save
An informational dialog box appears while file is being written and then the Preview menu will appear again
IC50
Please wait. Saving file IC50…
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 16: Preview
Select Insert
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 17: Select Next Pipetting Step (Aspirate to remove excess volume in the last column of the Serial
Dilution Plate)
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 18: Select Aspirate Labware Position
Selected stage from last command will be highlighted in Blue
Since last stage is already highlighted, Select Next
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 19: Confirm Labware on Stage 3. Labware is already highlighted.
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 20: Select Aspirate Parameters Selections are similar to Serial Dilution Command Parameters
Select Advanced for two more menus of Additional Aspirate Parameters
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 21: Select additional Aspirate Parameters if required
Select More for the third menu of Aspirate selections
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 22: Third menu of Aspirate parameters. Select additional Aspirate Parameters if required
Select Next
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 23: Aspirate Command Preview
Select Save (keyboard will appear as shown previously to confirm file name). Select Insert to add next Step
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 24: Select Next Pipetting Step (Dispense to dispense excess volume in last column of Serial Dilution Plate)
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 25: The last stage chosen, Stage 3, will be highlighted when this screen first appears. Select a new stage, Stage 4, to Dispense
Select Next
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 26: Confirm Labware on Stage 4. Last selected Labware is already highlighted.
Select Next
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IC50 Serial Dilution/8-Channel Pipetting Procedure
Step 27: Select Dispense Parameters Selections are similar to Aspirate Command Parameters
Select Advanced for two more menus of Additional Dispense Parameters
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 28: Select additional Dispense Parameters if required
Select More for the third menu of Dispense selections
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 29: Third menu of Dispense parameters. Select additional Dispense Parameters if required
Select Next
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IC50 Serial Dilution/ 8-Channel Pipetting Procedure
Step 30: Preview
Select Execute Program to Run Procedure