Compound Light Compound Light MicroscopesMicroscopes

Parts IdentificationParts Identification

LensesLenses

Eyepiece – 10XEyepiece – 10X Objective LensesObjective Lenses

– Low power – 4X * 10X = 40X Low power – 4X * 10X = 40X redred– Medium power – 10X *10X = 100X Medium power – 10X *10X = 100X

yellowyellow– High power – 40X * 10X = 400X High power – 40X * 10X = 400X blueblue

Proper HandlingProper Handling

Carry by the base and arm – two Carry by the base and arm – two hands!hands!

Set down gentlySet down gently Do not touch lensesDo not touch lenses

FocusingFocusing

Adjust light with dialAdjust light with dial– 1 = lowest light 5 = highest light1 = lowest light 5 = highest light

Focus first with low power and coarse Focus first with low power and coarse adjustmentadjustment

Then focus at medium power with fine Then focus at medium power with fine adjustmentadjustment

Last focus at high power with fine Last focus at high power with fine adjustmentadjustment

Use of coarse adjustment at medium Use of coarse adjustment at medium or high power will result in an “F”or high power will result in an “F”

Drawing Field of ViewDrawing Field of View

3. Description of specimen4. Magnification

1. Draw circle for field of view

2. Draw specimen to scale

Clean UpClean Up

Low power objectiveLow power objective Eyepiece over armEyepiece over arm Wrap cord neatly around microscopeWrap cord neatly around microscope Rinse and dry slides and coverslipsRinse and dry slides and coverslips Nobody leaves until all microscopes Nobody leaves until all microscopes

are properly cleaned up are properly cleaned up