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Page 1: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction

by Tashera E. Perry, Minjung Song, Daryl J. Despres, Soo Mi Kim, Hong San, Zu-Xi Yu, Nalini Raghavachari, Jurgen Schnermann, Richard O. Cannon, and Donald Orlic

Cardiovasc ResVolume ():cvp215

July 21, 2009

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].

Page 2: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

(A) Circulating white blood cells were analysed by flow cytometry to determine the percent chimerism.

Tashera E. Perry et al. Cardiovasc Res 2009;cvr.cvp215

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].

Page 3: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

(A) EGFP expression was detected by western blot analysis in heart, kidney, and spleen of EGFP+ controls and BM-transplanted eNOS−/− mice but not in eNOS−/− control mice.

Tashera E. Perry et al. Cardiovasc Res 2009;cvr.cvp215

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].

Page 4: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

Confocal microscopy of conducting arteries in heart, kidney, liver, and spleen in EGFP+ control mice are seen in (A–D).

Tashera E. Perry et al. Cardiovasc Res 2009;cvr.cvp215

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].

Page 5: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

Confocal microscopy of conducting arteries in heart, kidney, liver, and spleen in EGFP+ control mice are seen (A–D).

Tashera E. Perry et al. Cardiovasc Res 2009;cvr.cvp215

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].

Page 6: Bone marrow-derived cells do not repair endothelium in a mouse model of chronic endothelial cell dysfunction by Tashera E. Perry, Minjung Song, Daryl J.

Confocal microscopic images of several aortas are seen in (A–E).

Tashera E. Perry et al. Cardiovasc Res 2009;cvr.cvp215

Published on behalf of the European Society of Cardiology. All rights reserved. © The Author 2009. For permissions please email: [email protected].


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