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Page 1: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

BIO107Introduction to Biotechnology

Dr. Ty C.M. [email protected]

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Page 2: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Science Versus ReligionReligion is based on faith in the

absence of evidence.

Science is based on evidence that leads to belief.

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Page 3: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

What is Chemistry?

What is Biology?

What is Biotechnology?

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Page 4: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Sodium Chlorine Sodiumchloride

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Page 5: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Nucleus

Protons (+ charge)determine element

Neutrons (no charge)determine isotope Atom

Electrons (– charge) form negative cloudand determinechemical behavior

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Page 6: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Reactants Reaction Products

2 H2 O2 2 H2O

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Page 7: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

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Page 8: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Order

Evolutionary adaptation

Responseto theenvironment

ReproductionGrowth anddevelopment

Energyprocessing

Regulation

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Page 9: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

The biosphere

Communities

Populations

Organisms

Ecosystems

Organs and organ systems

Cells

Cell

Organelles

Atoms

MoleculesTissues

10 µm

1 µm

50 µm

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Page 10: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

1 µmOrganelles

Nucleus (contains DNA)

Cytoplasm

Membrane

DNA(no nucleus)

Membrane

Eukaryotic cellProkaryotic cell

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Page 11: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Nucleus DNA

Cell

Nucleotide

(a) DNA double helix (b) Single strand of DNA11

Page 12: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Classes of Biological Macromolecules

• Lipids• Polysaccharides• Proteins• Nucleic Acids

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Page 13: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Centrifuge

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Page 14: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Vortexer

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Page 15: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Tubes

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Page 16: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Racks

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Page 17: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Gel Trays

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Page 18: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Power Supplies

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Page 19: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Pipettes

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Page 20: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Pipettors

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Page 21: BIO107 Introduction to Biotechnology Dr. Ty C.M. Hoffman bio107hoffman@gmail.com

Tools of Biotechnology: Heat Blocks

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